Paracoccus hakeae

Ellenrieder, Natalia Von, Watson, Gillian W., Kinnee, Scott A., Franco, José C. & Mazzeo, Gaetana, 2016, Paracoccus leucadendri Mazzeo & Franco in Mazzeo, Franco & Russo, 2009, a junior synonym of Paracoccus hakeae (Williams, 1985) comb. nov. (Coccomorpha: Pseudococcidae), Zootaxa 4093 (4), pp. 552-558 : 556

Genetic distances and generic placement of Ph. hakeae

Molecular analysis showed the COI sequences of 610 base pairs of the Australian and Californian specimens to be identical. The sequenced COI portion of Phenacoccus hakeae differs by 11–15 % from similar sequences of other Phenacoccus species ( Ph. aceris (Signoret) , Ph. avenae Borchsenius , Ph. baccharidis Williams , Ph. madeirensis Green , Ph. parvus Morrison , Ph. peruvianus Granara de Willink in: Granara de Willink & Szumik, Ph. solani Ferris , Ph. solenopsis Tinsley ) and by 9–14 % from those of Paracoccus species ( Pa. burnerae (Brain) , Pa. ferrisi Ezzat & McConnell or nr., Pa. gillianae von Ellenrieder & Stocks, Pa. marginatus Williams & Granara de Willink) available on GenBank and in the CSCA dataset.

The TaxonID tree-based identification tool in BOLD (Ratnasigham & Hebert 2013) shows Ph. hakeae grouping with Crisicoccus matsumotoi (Siraiwa) (8.5–10 % difference) known from South-East Asia and the Oriental region, and Vryburgia transvaalensis (Brain) (9.9–10 % difference) from southern Africa.

Our Neighbor-Joining analysis performed using available sequences in the CSCA database and some selected sequences from GenBank shows Ph. hakeae grouping with members of the subfamily Pseudococcinae with good support, and in a subgroup including Balanococcus takahashii McKenzie and Crisicoccus matsumotoi with low support. It also shows the genus Phenacoccus forming a separate grouping ( Fig. 1 ), as is expected because Phenacoccus belongs to the subfamily Phenacoccinae (Hardy et al. 2008).

Figures

FIGURE 1. Optimal tree inferred using the Neighbor-Joining method inferred from 550 replicates. Shown next to the branches is the percentage of replicate trees in which the associated taxa clustered together in the bootstrap test. The tree is drawn to scale, with branch lengths in the same units as those of the genetic distances used to infer the tree. Genetic distances were computed using the p-distance method and are in the units of the number of base differences per site.