Dothiorella sarmentorum (Fr.) A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97: 522. (2005).

Li, Wen-Li, Liang, Rui-Ru, Dissanayake, Asha J. & Liu, Jian-Kui, 2023, Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China, MycoKeys 97, pp. 71-116 : 71

Dothiorella sarmentorum (Fr.) A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97: 522. (2005).

Fig. 13

Sphaeria sarmentorum Fr., K. svenska Vetensk-Acad. Handl. 39: 107. 1818. Basionym.

Diplodia sarmentorum ≡ Diplodia sarmentorum (Fr.) Fr., Summ. veg. Scand. (Stockholm) 2: 417. 1849.

Diplodia pruni = Diplodia pruni Fuckel, Jahrb. Nassauischen Vereins Naturk., 23-24: 169. 1870 [1869].

Botryosphaeria sarmentorum = Botryosphaeria sarmentorum A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97: 522. 2005.

Description.

Saprobic on decaying branches of Pistacia chinensis . Sexual morph: Not observed. Asexual morph: Conidiomata 278-338 × 240-280 μm (x̄ = 308 × 260 μm, n = 10), immersed, erumpent, forming split-like opening on the host, gregarious, globose to subglobose, dark brown to black, unilocular or multilocular, papillate, ostiolate. Ostiole 52-57 μm diam., conical or circular, centrically located. Peridium 28.5-44 μm, comprising 5-8 layers of thick-walled, dark brown to hyaline cells arranged in a textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 2.5-3.5 × 6-9 μm (x̄ = 3 × 7.5 μm, n = 20), holoblastic, discrete, cylindrical, hyaline, smooth, indeterminate, proliferating at the same level giving rise to periclinal thickenings, or rarely proliferating percurrently to form one or two close, indistinct annellations. Conidia 21.5-24 × 9-10 μm (x̄ = 22.5 × 9.5 μm, n = 30), L/W ratio = 2.4, ellipsoid to obovoid, with rounded ends, initially hyaline and aseptate becoming pigmented brown and 1-septate often while still attached to conidiogenous cell, brown walled, slightly constricted at the septum.

Culture characteristics.

Conidia germinate on PDA within 12 h. Colonies growing on PDA, reaching a diameter of 4 cm after three days at 25 °C, effuse, velvety, with entire to slightly undulate edge. Surface initially white and later turning dark olivaceous from the surrounding of the colony and dark gray in reverse.

Materials examined.

China, Sichuan Province, Chengdu City, Pidu District , 30°19'57"N, 103°59'47"E, elevation 442 m, on dead branches of Pistacia chinensis , 19th March 2021, W.L Li, 072 (HUEST 22.0077), living culture UESTCC 22.0076; ibid., Guangyuan City , Qingchuan County, 32°40'38"N, 105°28'57"E, elevation 638 m, 20th April 2021, W.L Li, A189 (HUEST 22.0078), living culture UESTCC 22.0077. Additional sequences: LSU: OQ164837 (UESTCC 22.0076), OQ164838 (UESTCC 22.0077) .

Notes.

Dothiorella sarmentorum was introduced by Phillips et al (2005) with both asexual and sexual morphs. Recently, nine Dothiorella species ( Do. californica , Do. iberica , Do. italica , Do. guttulata , Do. omnivora , Do. parva , Do. sempervirentis , Do. symphoricarpicola , Do. vidmadera ) were synonymized under Do. Sarmentorum by Zhang et al. (2021) based on phylogenetic analyses. Two isolates obtained in the present study clustered with the group of Do. sarmentorum taxa in the phylogenetic analyses (Fig. 4 ).

Figures

Figure 4. Phylogram generated from RAxML analysis based on combined ITS, tef 1 and tub 2 sequence data of Dothiorella isolates. The tree was rooted to Neofusicoccum luteum (CBS 562.92 and CMW 41365). The ML (≥ 75 %) and BI (≥ 95 %) bootstrap supports are given near the nodes, respectively. Isolates from this study are marked in red and ex-type strains are marked in bold.

Figure 13. Dothiorella sarmentorum (HUEST 22.0077, new host record) a, b appearance of conidiomata on natural substrate c vertical section of conidioma d-g conidiogenous cells and developing conidia h-l brown conidia. Scale bars: 50 μm (c); 10 μm (d-l).