Aspergillus doliiformis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Aspergillus doliiformis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 5

Etymology.

Referring to the lantern shape of conidia.

Type.

Hainan Province, Haikou City, Haidian Campus of Hainan University 20°05'76"N, 110°32'91"E, from soil, 28 Aug 2019, Z.Y. Zhang (HMAS 351867 holotype designated here, ex-type living culture CGMCC 3.20772 = GZUIFR 21.885); ibid., GZUIFR 21.883.

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 23-26 mm diam., white (1A1), felty, fluffy, margin slightly undulate; reverse pale yellow (1A3) to white (1A1) from centre to margin. Colony on MEA 16 mm diam., white (1A1) at margin, olive yellow (2D8) at centre, felty, margin entire; reverse oak brown (5D6) to brownish-grey (5D2) from centre to margin. Colony on OA 26-27 mm diam., white (1A1), flocculent, margin entire, producing a diffusible faint yellow pigment; reverse greyish-yellow (2B4).

Hyphae hyaline, septate, smooth, branched, 1.0-3.5 μm wide. Conidiophores solitary phialides borne laterally or terminally on vegetative hyphae. Phialides mono- to polyphialidic, hyaline, ampulliform, campaniform, cylindrical or tapering with an enlarged base, sometimes curved irregularly, occasionally branched, neck cylindrical or broadly tapering, sometimes extending sympodially from the necks, 2.5-18.0 × 1.0-5.0 µm. Conidia borne solitary or formed in long chains, hyaline, smooth or roughened, lantern, subglobose to globose, obpyriform, 3.0-5.0 × 2.5-4.5 µm (av. 4.2 × 3.4 μm, n = 50). Sexual morph not observed.

Additional specimens examined.

China: Hainan Province, Haikou City , Hainan General Hospital 20°00'57"N, 110°28'78" E, from soil, 28 Aug 2019, Z.Y. Zhang, GZUIFR 21.884; Haikou People’s Park, soil, 28 Aug 2019, Z.Y. Zhang, GZUIFR 21.886 .

Notes.

Aspergillus doliiformis represents a new lineage within the subgenus Polypaecilum Polypaecilum , series Canini , forming a fully supported clade (ML = 100%; PP = 1.0). A. doliiformis is phylogenetically closely related to A. limoniformis and A. cylindricus . However, A. doliiformis can be distinguished from A. limoniformis by their sequence similarity (93% 502/539; 99% 847/853; 87% 370/423; 91% 762/834; 92% 724/791 similarity of ITS, LSU, TUB, RPB2 and TSR1 in A. limoniformis CGMCC 3.19323). Morphologically, the conidia of A. limoniformis are limoniform or subglobose, rather than lantern, subglobose to globose, obpyriform in A. doliiformis ( Zhang et al. 2021a). In addition, the distinction between A. doliiformis and A. cylindricus is shown in the notes of A. cylindricus .

Figures

Figure 5. Aspergillus doliiformis (from ex-holotype CGMCC 3.20772) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-k phialides and conidia. Scale bars: 10 µm (d-k).