Colacogloea pararetinophila C.Y. Chai & F.L. Hui, 2024

Lu, Yun-Feng, Chai, Chun-Yue & Hui, Feng-Li, 2024, Two new phyllospheric species of Colacogloea (Colacogloeaceae, Pucciniomycotina) identified in China, MycoKeys 101, pp. 81-94 : 81

Colacogloea pararetinophila C.Y. Chai & F.L. Hui sp. nov.

Fig. 2B

Etymology.

The specific epithet " pararetinophila " refers to its phylogenetic similarity to C. retinophila .

Typus.

China, Henan Province, Neixiang County, Baotianman Nature Reserve, Mayigou (33°30′44″N, 111°55′47″E) in phylloplane from undetermined leaf, October 2021, W.T. Hu and R.Z. Qiao, NYNU 2110393 (holotype CICC 33533T preserved as a metabolically inactive state, culture ex-type JCM 35724 and GDMCC 2.268).

Description.

On YM agar, after two weeks at 20 °C, the streak culture is cream, butyrous, and smooth. The margin is entire. In YM broth, after 7 d at 20 °C, cells are ovoid or ellipsoidal, 2.0-2.6 × 2.8-4.2 μm and single, budding is polar. After 1 mo at 20 °C, a ring and sediment are present. In Dalmau plate culture on corn meal agar, hyphae and pseudohyphae are not formed. Sexual structures are not observed for individual strains and strain pairs on PDA, CM agar and YCBS agar for two months. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, trehalose (weak), salicin (weak), D-xylose, D-arabinose, 5-keto-D-gluconate, D-ribose, ethanol, glycerol, ribitol, D-mannitol, D-glucitol, succinate (weak), citrate (weak), D-gluconate, D-glucosamine, 2-keto-D-gluconateare, and glucono-1,5-lactone assimilated as sole carbon sources. Inulin, sucrose, raffinose, melibiose, galactose, lactose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, L-sorbose, L-rhamnose, L-arabinose, methanol, erythritol, galactitol, myo-inositol, DL-lactate, and D-glucuronate are not assimilated. Nitrate, nitrite, ethylamine, and L-lysine are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 37 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China, Henan Province, Neixiang County, Baotianman Nature Reserve, Tianmanpubu (33°30′26″N, 112°02′28″E) in phylloplane from undetermined leaf, October 2021, W.T. Hu and R.Z. Qiao, NYNU 2110421 and October 2022, J.Z. Li, NYUN 2211185.

GenBank accession numbers.

Holotype NYNU 2110393T (ITS: OM014194, D1/D2: OM014193); additional strains NYNU 2110421 (ITS: OR727348, D1/D2: OR727347) and NYUN 2211185 (ITS: OR727352, D1/D2: OR727351).

Note.

Phylogenetic analyses revealed that C. pararetinophila sp. nov. has a close relationship with C. retinophila with high support values (100 MLBP/1 BPP; Fig. 1 ). C. pararetinophila sp. nov. can be physiologically differed from its closest relative C. retinophila ( Sampaio 2011) in the ability to assimilate salicin and D-ribose and inability to assimilate D-glucuronate. In addition, C. pararetinophila sp. nov. can grow in 0.1% cycloheximide while C. retinophila cannot (Table 3 ).

Figures

Figure 1. Maximum likelihood (ML) phylogram of Colacogloea species based on combined ITS and LSU sequence data. Udeniozyma ferulica CBS 7416 T was used as the outgroup. Branches are labeled with MLBS ≥ 50 % and BPP ≥ 0.95. New strains described in this study are shown in bold.

Figure 2. Vegetative cells of Colacogloea celtidis sp. nov. NYUN 2210184 T (A) and Colacogloea pararetinophila sp. nov. NYNU 2110393 T (B) following growth in YM broth for 7 days at 20 ° C. Scale bars: 10 μm.