Phoneutria depilata (Strand, 1909) sp. reval.

Hazzi, Nicolas A. & Hormiga, Gustavo, 2021, Morphological and molecular evidence support the taxonomic separation of the medically important Neotropical spiders Phoneutria depilata (Strand, 1909) and P. boliviensis (F. O. Pickard-Cambridge, 1897) (Araneae, Ctenidae), ZooKeys 1022, pp. 13-50: 13

publication ID

http://dx.doi.org/10.3897/zookeys.1022.60571

publication LSID

lsid:zoobank.org:pub:C0A606CC-48EC-43CB-A7F3-0D62D8125F13

persistent identifier

http://treatment.plazi.org/id/7D466905-93B1-58CC-B065-79F83CF10620

treatment provided by

ZooKeys by Pensoft

scientific name

Phoneutria depilata (Strand, 1909) sp. reval.
status

 

Phoneutria depilata (Strand, 1909) sp. reval.   Figs 1A, B View Figure 1 , 4C, D View Figure 4 , 7A-C View Figure 7 , 8A, B View Figure 8 , 9B, D View Figure 9 , 10C, D View Figure 10

Ctenus depilatus   Strand, 1910. Holotype male from Colombia ( ZMB 30615 View Materials , examined). Valerio 1983: 101, fig. 2 (female).  

Ctenus peregrinoides   : Strand, 1910: 318 (syntypes: two females from Guatemala, in ZMB 30717 View Materials , not examined). New synonymy   .

Phoneutria depilata   : Schmidt, 1954: 417-418.

Phoneutria colombiana   Schmidt, 1956: 418; 1956: 28 (female holotype from Colombia, in SMF, not examined). New synonymy.

Phoneutria boliviensis   : Simó & Brescovit, 2001: 74 (as senior synonym of P. depilata   ); Rozwałka, Rutkowski and Bielak-Bielecki 2017: 61, fig. 1b, c (female); Hazzi et al. 2018: 112, fig. 10D (male).

Phoneutria cf. boliviensis   : Cathrine & Longhorn, 2017: 13, figs 1-6 (female).

Comments.

In their revision of Phoneutria   Simó and Brescovit (2001) distinguished the Amazonian specimens of Phoneutria boliviensis   from the specimens from Colombia and Central America (which we identify now as P. depilata   ) based on the epigynal morphology: "In specimens from Central America to Colombia it is triangular, with a wide base and a narrow apex, but in specimens from Ecuador to Bolivia the apex is more rounded". Based on the fact that Simó & Brescovit were able to distinguish these epigynal morphological differences among these two Phoneutria   species and that the only species of Phoneutria   in the trans-Andean region is P. depilata   , we suggest that Ctenus peregrinoides   (from Guatemala) is a junior synonym of P. depilata   . Strand described Ctenus signativenter   in 1909 based on immature syntypes from Paramba, Ecuador (one male and two female syntypes, all immatures, 3500 ft, 28 April 1898, Rosenberg leg., in ZMB 306 View Materials , not examined). We have designated Ctenus signativenter   as a nomen dubium because the exact identity of this species cannot be ascertained with immature specimens, but we note that the type locality suggests that the C. signativenter   syntypes belong to P. depilata   . Based on the epigynal morphology ( Schmidt 1956, fig.3), we synonymize Phoneutria colombiana   with P. depilata   . Both Ctenus peregrinoides   and Phoneutria colombiana   had been synonymized with P. boliviensis   by Simó and Brescovit (2001)   .

Other material examined.

Nicaragua: Región Autónoma de la Costa Caribe Sur: one female, Escondido River (12.1065°N, 84.0256°W, 10 m), 12.VII.1892, C.W. Richmond ( USNM) GoogleMaps   . Panama: Panama: one male, Pearls Island, San Jose (8.270219°N, - 79.112038°W, 30 m), 02.IV.1944, J.P. Morrinson ( USNM) GoogleMaps   ; Bocas del Toro: one female, Changinola , El Silencio (9.3845°N, 82.5356°W, 20 m), E. Marrango ( USNM) GoogleMaps   , one male and one female, Gamboa (9.1176°N. 79.6959°W, 50 m), 05.XIII.2018, N. Hazzi and S. Maneses ( MCZ IZ 162179 View Materials ) GoogleMaps   ; Chiriquí: one male, one female, Puerto Amuelles (8.2841°N, 82.8691°W, 10 m), 25.VII.2018, N. Hazzi, J. Bernal, T. Rios ( MCZ IZ 162180 View Materials ) GoogleMaps   . Costa Rica: Alajuela: one male and one female, San Ramón, Muelle San Carlos (10.4335°N, 84.5622°W, 990 m) ( MZUCR) GoogleMaps   ; one male, Canalete, Upala (10.8358°N, 85.0437°W, 950 m), 25.XI. 1979 ( MZUCR) GoogleMaps   , two females and two males, San Carlos, Peje Viejo (9.644°N, 82.7516°W), F. Garray ( MZUCR) GoogleMaps   , 00. X.1999; Limón: one female, Guapiles (10.2217°N, 83.7705°W, 450 m), 30.IX.1977 ( MZUCR) GoogleMaps   ; one female, Batan (10.0842°N, 83.3364°W), 12.VIII.1984, Federico Muñoz ( MZUCR) GoogleMaps   , Reserva Biológica Hitoy Cerere (9.647°N, 83.0709°W); one female, Talamanca, Amumbri de Bratsi (9.6501°N, 82.7542°W) ( MZUCR) GoogleMaps   ; Puntarenas: one female, Rincon de Osa (8.6986°N, 83.4876°W, 20 m), 00. III.1967, C. Valerio ( MZUCR) GoogleMaps   ; one male, Conte, Casa de la Guardia Rural (8.443°N, 83.0401°W, 990 m), 14.VII.1984; one female and one male Cirenas (9.7199°N, 85.2119°W, 10 m), 00. VI.2018, N. Hazzi ( MCZ IZ 162181 View Materials ) GoogleMaps   , Cartago: one female, Turrialba, Bajo Pacuare (9.862°N, 83.5203°W, 730 m), 25.IV.1983, F. Calderón ( MZUCR) GoogleMaps   ; Heredia: one juvenile, Sarapiqui, Reserva Tirimbina (10.4164°N, 84.1199°W, 160 m), 10. VI.2019, N. Hazzi; one male, San Isidro (10.0182°N, 84.0551°W, 1300 m) ( MCZ IZ 162183 View Materials ) GoogleMaps   . Ecuador: Esmeraldas: Esmeraldas, Caimito (0.7005°N, 80.0741°W, 10 m), 1.10.2019, N. Hazzi ( MCZ IZ 162184 View Materials ) GoogleMaps   . Colombia: Chocó: one female, Bahía Solano, Ciudad Mutis (6.2186°N, 77.4075°W, 5 m), 5. V.1973 ( ICN-AR) GoogleMaps   ; one juvenile, Acandí, Capurgana (8.6338°N, 77.3503°W, 15 m), 08.X.2007, C. Duran ( MPUJ) GoogleMaps   ; Cundinamarca: Fusagasugá (4.3439°N, 74.3678°W, 1600 m), 00. XII.2001 ( ICN-AR-5258); Yacupi, vereda La Oscura (5.45°N, 74.35°W, 1190 m), 03.I.2000 ( ICN-AR-907); Quipile, Vereda el Trigo (4.7455°N, 74.5341°W, 1300 m); 28.V.2000 ( ICN-AR-908); Pandi, vereda El Caucho (4.1911°N, 74.4875°W, 910 m), 20.IX.2000 ( ICN-AR-903); La Mesa (4.6333°N, 74.4666°W, 1080 m), 16.VI.1983 ( ICN-AR-343); Nilo, Pueblo Nuevo (4.3166°N, 74.6333°W, 480 m), 12.I.1980 ( ICN-AR-303) GoogleMaps   ; Santander: Chima, El Rodeo (6.3458°N, 73.3736°W, 113 m); 03.I.1970 ( ICN-AR-315); one female, Suaita (6.10°N, 73.45°W, 1550 m), 10. V.1998 ( ICN-AR-5261) GoogleMaps   ; Antioquia: one female, Urabá, Apartadó (7.8856°N, 76.6347°W, 20 m), 00. VII.2003 ( ICN-AR-5259); one female, Urabá, Turbó (8.0981°N, 76.7317°W, 20 m) ( ICN-AR-5260); one female, Urabá, Chigorodó (7.6769°N, 76.6864°W, 20 m), 00. IX.2003 ( ICN-AR-5262) GoogleMaps   ; Cauca: one male, PNN. Gorgona Island (2.98°N, 78.1825°W, 5 m), 00. XII.2003 ( ICN-AR-5263); one female and five males, Caloto, vereda Morales (3.0369°N, 76.4116°W, 1100 m), 00. X.2009, N. Muriel ( MUSENUV) GoogleMaps   ; Valle del Cauca: one female and one male, Cali, El Aguacatal (3.4617°N, 76.5560°W, 1000 m), N.Hazzi ( MUSENUV); one female, Cali (3.4616°N, 76.5560°W, 1000 m), 20.X.1982 ( MUSENUV); one female GoogleMaps   , same locality 00. X.1986 ( MUSENUV); one male, Cali, Barrio El Refugio (3.4372°N, 76.5225°W, 1000 m), 00. XI.1995 ( MUSENUV) one female, Dagua , El Palmar (3.6033°N, 76.6463°W, 1300 m), 27.IX.1994 ( MUSENUV); one female, Roldanillo (4.4147°N, 76.1547°W, 950 m) ( MUSENUV); KM 30, El Carmen (3.566°N, 76.6475°W, 1400 m); 10.XII.2008, N. Hazzi ( MUSENUV); one male, Buga, Liceo de los Andes (3.8833°N, 76.2986°W, 950 m), 12.XII.2009, N. Hazzi ( MUSENUV), one female, Buenaventura, Reserva Natural San Cipriano (3.8833°N, 76.9166°W, 180 m), 00. II.2012, N.Hazzi and J. Moreno ( MUSENUV) GoogleMaps   ; Boyaca: Puerto   Boyaca, Puerto Romero, vereda Los Quinchos (5.8375°N, 74.3408°W, 160 m) GoogleMaps   ; Risaralda: one female, Pereira (4.8133°N, 75.6961°W, 1400 m) ( MUSENUV); one male, Balboa (4.9517°N, 75.9572°W, 1400 m); 10.XI.1998 ( ICN-AR-5264) GoogleMaps   ; Quindío: one female, Montenegro, La Tebaida (4.5542°N, 75.7181°W, 1200 m), 27.I.2010, N. Hazzi ( MUSENUV); one female, Montenegro , Pueblo Tapado (4.5178°N, 75.7847°W, 1250 m), 00. X.2004 ( MUSENUV) GoogleMaps   ; Caldas: Samaná, Norcasia, Carrisa (5.5666°N, 74.8833°W, 600 m); 10.X.1992 ( ICN-AR342) GoogleMaps   .

Diagnosis.

Males of P. depilata   resemble those of P. boliviensis   by the truncated apex of the RTA (Fig. 9C, D View Figure 9 ), but differ from this and the remaining Phoneutria   species by the lateral pronounced projection of the locking lobes visible in ventral view (Fig. 9B View Figure 9 ). In addition, males present an embolus with an internal bulge which is absent in P. boliviensis   ; and a much larger tegulum (Figs 7B View Figure 7 , 9B View Figure 9 ). Females of P. boliviensis   also resemble those of P. depilata   by the general configuration of the epigynum but differ by the narrow area of the EMF (Fig. 8A View Figure 8 ), copulatory ducts slightly sclerotized (Fig. 8A View Figure 8 ), and large spermatheca heads (Figs 8B View Figure 8 , 10D View Figure 10 ). In addition, both males and females of P. depilata   can be distinguished from P. boliviensis   and the remaining Amazonian species ( P. perty   and P. fera   ) by the four conspicuous series of yellow dots in the ventral side of the abdomen (this character is also present in Phoneutria eickstedtae   Martins & Bertani, 2007).

Description.

Male (from Puerto Amuelles, Chiriquí, Panama, MCZ IZ 162180 View Materials -1). Coloration (Figs 1B View Figure 1 , 4C, D View Figure 4 ): Carapace brown with a longitudinal black line. Ocular area with brown setae and back oblique band from PLE to anterior dorsal shield of prosoma edge. Chelicerae brown with reddish setae. Sternum, endites and labium yellowish-brown. Abdomen yellow-brown dorsally, with yellow dots; ventrally dark brown with four conspicuous series of yellow dots. Total length 23.21. Carapace 12.38 long and 9.94 wide, eye diameters: AME 0.46, ALE 0.34, PME 0.55, PLE 0.55. Clypeal height 0.45, sternum 5.03 long, 4.65 wide; labium 1.62 long, 1.62 wide. Sternum 2.58 long and 2.50 wide, labium 1.99 long and 2.15 wide, endites 2.93 long and 1.70 wide. Leg measurements: I: femur 14.65, patella 5.77, tibia 15.75, metatarsus 14.31, tarsus 3.93, total 54.41; II: 13.72, 5.19, 13.85, 12.63, 3.30, total 48.69; III, 11.12, 4.95, 10.21, 10.09, 3.17, total 39.54; IV 13.56, 4.86, 13.25, 16.35, 3.96, total 51.98. Leg spination: I tibia v2-2-2-2-2, d1-1-1, p0-1-0, r1-1-0, metatarsus v2-2-2, p1-0-0 r1-0-0, II tibia v-2-2-2-2-2, d1-1-1, p1-1-0, r1-1-0, metatarsus v2-2-2, p1-0-0 r1-0-0, III v2-2-2, d1-1-1, p1-0-1-0, r1-0-1-0, metatarsus v2-2-2-2, p1-1-2, r1-1-2, IV tibia v2-2-2, d1-1-1, p1-0-1-0, r1-0-1-0, metatarsus v2-2-2-2, d0-1-0, p1-1-2, r1-1-2. Palp: RTA small and truncated at the apex (Figs 7C View Figure 7 , 9D View Figure 9 ); embolus curve with internal bulge (Figs 7B View Figure 7 , 9B View Figure 9 ); cup-shaped median apophysis constrain at the base (Figs 7B View Figure 7 , 9B View Figure 9 ); conductor membranous, hyaline and C-shaped (Figs 7B View Figure 7 , 9B View Figure 9 ); tegulum large with probasal rounded projection (Figs 7B View Figure 7 , 9B View Figure 9 ).

Female (from Puerto Amuelles, Chiriquí, Panama, ( MCZ IZ 162180 View Materials -2). Coloration (Figs 1B View Figure 1 , 4C, D View Figure 4 ): Carapace brown with a longitudinal black line. Ocular area with brown setae and back oblique band from PLE to anterior dorsal shield of prosoma edge. Chelicerae brown with reddish setae. Sternum, endites and labium yellowish-brown. Abdomen yellow-brown dorsally, with yellow dots; ventrally dark brown with four conspicuous series of yellow dots. Total length 25.77. Carapace 12.56 long and 9.82 wide, eye diameter: AME 0.47, ALE 0.36, PME 0.60, PLE 0.65. Clypeal height 0.89, sternum long 5.15 and 4.76 wide, endites 3.89 long and 2.30 wide, labium 1.33 long and 1.59 wide. Leg measurements: I: femur 13.43, patella 5.09, tibia 12.78, metatarsus 9.99, tarsus 3.33, total 44.62; II, 11.22, 5.00, 11.57, 9.30, 3.00, total 40.09; III 7.83, 4.00, 7.89, 7.60, 2.50, total 29.83; IV 12.00, 4.62, 9.83, 12.72, 3.15, total 42.32. Leg spination: tibia I-II v2-2-2-2-2, metatarsus I-II v2-2-2-2-2; III tibia v2-2-2, d1-1-1, p1-0-1-0, r1-0-1-0; metatarsus v2-2-2-2, p1-1-2, r1-1-2; IV tibia v2-2-2, d1-1-1, p1-0-1-0, r1-0-1-0, metatarsus v2-2-2-2, d0-1-0, p1-1-2, r1-1-2. Epigynum (Figs 6A View Figure 6 , 10C View Figure 10 ): middle field convex with straight edges, anteriorly divergent and posteriorly convergent; lateral field with lateral apophysis. Vulva (Figs 6B View Figure 6 , 10D View Figure 10 ): copulatory ducts slightly sclerotized, enlarged spermatheca heads, fertilization ducts small and posteriorly located.

Variation.

Males (n = 5): Total length 21.00-26.37, carapace 11.26-13.75, femur I 13.27-15.84. Females (n = 8): Total length 25.77-34.00, carapace 12.56-15.00, femur I 13.43-14.36.

Distribution.

Trans-Andean region (0-1700 m) in Ecuador, Colombia, Panama, Costa Rica, Nicaragua, Honduras and Guatemala.

Natural history.

This species is found in disturbed habitats associated with both dry and humid tropical forests (0-1700 m), usually on the ground with sparse litter and low vegetation ( Hazzi 2014). The range of eggs per egg sac is 430-1300, and spiderlings emerge 28-34 days after the egg sacs are produced. Sexual maturity occurs after 14-17 molts, and spiders mature 300-465 days after emerging from the egg sac ( Hazzi 2014). Valenzuela-Rojas et al. (2020) reported that P. depilata   is an euryphagous predator with a broad diet made up predominantly of arthropods and to a lesser extent of small vertebrates ( Gekkonidae   , Hylidae   , and Sphaerodactylidae   ). There are human bite records of this species reported in Costa Rica and in banana plantations in Colombia ( Florez et al. 2003). All the cases reported have occurred with adults, and most of them have presented mild to moderate envenomation symptoms, with only one patient presenting severe symptoms such as renal failure ( Florez et al. 2003). Estrada-Gomez et al. (2015) partially characterized the venom of this species, detecting Ctenitoxin-Pb48 and Ctenitoxin-Pb53, which showed a high homology with other Ctenitoxins (family Tx3) from P. nigriventer   , P. keyserlingi   and P. reidyi   affecting voltage-gated calcium receptors (Cav 1, 2.1, 2.2 and 2.3) and NMDA-glutamate receptors. Valenzuela-Rojas et al. (2019) found that the venom of P. depilata   was significantly more effective on vertebrate (geckos) than invertebrate (spiders) prey in both LD50 and immobilization time. In addition, males had slightly more toxic venom (LD50) to geckos and much more toxic venom to spiders when compared to females ( Valenzuela-Rojas et al. 2019). For two periods, March to May and October to November, adult males and females with egg sacs are always found in homes in the Inter-Andean Cauca Valley of Colombia. This likely indicates two reproductive peaks that coincided with the two rainy seasons during those same periods (N. Hazzi, unpub. data).

ZMB

Museum f�r Naturkunde Berlin (Zoological Collections)

SMF

Forschungsinstitut und Natur-Museum Senckenberg

USNM

Smithsonian Institution, National Museum of Natural History

MCZ

Museum of Comparative Zoology

IZ

Instituto de Zoologia

T

Tavera, Department of Geology and Geophysics

VI

Mykotektet, National Veterinary Institute

V

Royal British Columbia Museum - Herbarium

PL

Západoceské muzeum v Plzni

Kingdom

Animalia

Phylum

Arthropoda

Class

Arachnida

Order

Araneae

Family

Ctenidae

Genus

Phoneutria

Loc

Phoneutria depilata (Strand, 1909) sp. reval.

Hazzi, Nicolas A. & Hormiga, Gustavo 2021
2021
Loc

Phoneutria colombiana

Schmidt 1956
1956
Loc

Ctenus depilatus

Strand 1909
1909