Colacogloea celtidis C.Y. Chai & F.L. Hui, 2024

Lu, Yun-Feng, Chai, Chun-Yue & Hui, Feng-Li, 2024, Two new phyllospheric species of Colacogloea (Colacogloeaceae, Pucciniomycotina) identified in China, MycoKeys 101, pp. 81-94 : 81

Colacogloea celtidis C.Y. Chai & F.L. Hui sp. nov.

Fig. 2A

Etymology.

The specific epithet " celtidis " refers to Celtis, the plant genus, from which the type strain was isolated.

Typus.

China, Henan Province, Neixiang County, Baotianman Nature Reserve, Getiaopa (33°29′07″N, 111°52′51″E), in phylloplane from leaf of Celtis bungeana , October 2022, J.Z. Li, NYUN 2210184 (holotype GDMCC 2.332T preserved as a metabolically inactive state, culture ex-type KCTC 37265 and CICC 33577).

Description.

On YM agar, after two weeks at 20 °C, the streak culture is cream, butyrous, and smooth. The margin is entire. In YM broth, after 7 d at 20 °C, cells are long cylindrical, 2.3-3.0 × 7.0-10.2 μm and single, budding is polar. After 1 mo at 20 °C, a ring and sediment are present. In Dalmau plate culture on corn meal agar, hyphae and pseudohyphae are not formed. Sexual structures are not observed for individual strains and strain pairs on PDA, CM agar and YCBS agar for two months. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, salicin, D-xylose (weak), D-arabinose, 5-keto-D-gluconate, ethanol (weak), glycerol, ribitol, D-mannitol, D-glucitol, succinate, D-gluconate, D-glucosamine (weak), 2-keto-D-gluconate, D-glucuronate, and glucono-1,5-lactone are assimilated as sole carbon sources. Inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, L-sorbose, L-rhamnose, L-arabinose, D-ribose, methanol, erythritol, galactitol, myo-inositol, DL-lactate, and citrate are not assimilated. Nitrate, nitrite (delayed and weak), ethylamine (delayed and weak), and L-lysine (delayed) are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China, Henan Province, Neixiang County, Baotianman Nature Reserve, Mayigou (33°30′44″N, 111°55′47″E) in phylloplane from undetermined leaf, October 2022, J.Z. Li, NYNU 221136.

GenBank accession numbers.

Holotype NYUN 2210184T (ITS: OP954665, D1/D2: OP954664); additional strain NYUN 221136 (ITS: OR727350, D1/D2: OR727349).

Note.

Phylogenetic analyses revealed that C. celtidis sp. nov. formed a single clade with high support (100 MLBP/1 BPP; Fig. 1 ). C. eltise sp. nov. can be physiologically differentiated from its closest known species C. armeniacae ( Wang et al. 2021) by its ability to grow in D-xylose and D-arabinose and inability to grow in trehalose and cellobiose (Table 3 ).

Figures

Figure 1. Maximum likelihood (ML) phylogram of Colacogloea species based on combined ITS and LSU sequence data. Udeniozyma ferulica CBS 7416 T was used as the outgroup. Branches are labeled with MLBS ≥ 50 % and BPP ≥ 0.95. New strains described in this study are shown in bold.

Figure 2. Vegetative cells of Colacogloea celtidis sp. nov. NYUN 2210184 T (A) and Colacogloea pararetinophila sp. nov. NYNU 2110393 T (B) following growth in YM broth for 7 days at 20 ° C. Scale bars: 10 μm.