Campylaimus amphidialis Fadeeva, Mordukhovich & Zograf, 2016

Holovachov, Oleksandr, 2019, New and known species of the genus Campylaimus Cobb, 1920 (Nematoda: Araeolaimida: Diplopeltidae) from North European marine habitats, Biodiversity Data Journal 7, pp. 46545-46545 : 46545

Campylaimus amphidialis Fadeeva, Mordukhovich & Zograf, 2016

Materials

Type status: Other material. Occurrence: catalogNumber: SMNH-177093 ; individualCount: 2; sex: 1 female, 1 male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Kristineberg-Lysekil ; minimumDepthInMeters: 53; maximumDepthInMeters: 53; verbatimLatitude: 58°15,73'N; verbatimLongitude: 11°26,10'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177094 ; individualCount: 2; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Kristineberg-Lysekil ; minimumDepthInMeters: 53; maximumDepthInMeters: 53; verbatimLatitude: 58°15,73'N; verbatimLongitude: 11°26,10'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177095 ; individualCount: 3; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 390; maximumDepthInMeters: 428; verbatimLatitude: 58°22,30'N; verbatimLongitude: 10°20,33'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 10; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177098 ; individualCount: 5; sex: 3 females, 2 males; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 139; maximumDepthInMeters: 153; verbatimLatitude: 58°34,19'N; verbatimLongitude: 10°38,20'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 12; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177099 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 8; maximumDepthInMeters: 15; verbatimLatitude: 58°15,09'N; verbatimLongitude: 11°27,54'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Muddy sand Type status: Other material. Occurrence: catalogNumber: SMNH-177102 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 180; maximumDepthInMeters: 216; verbatimLatitude: 58°28,16'N; verbatimLongitude: 10°37,04'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 11; habitat: Hard bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177103 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 352; maximumDepthInMeters: 374; verbatimLatitude: 58°19,18'N; verbatimLongitude: 10°29,34'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 10; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177104 ; individualCount: 2; sex: 1 female, 1 male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 232; maximumDepthInMeters: 240; verbatimLatitude: 58°27,40'N; verbatimLongitude: 10°33,56'E; Identification: identifiedBy: Oleksandr Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 12; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177105 ; individualCount: 3; sex: 2 males, 1 female; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 248; maximumDepthInMeters: 316; verbatimLatitude: 58°28,22'N; verbatimLongitude: 10°29,39'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 11; habitat: Mixed bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177106 ; individualCount: 1; sex: female; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 390; maximumDepthInMeters: 428; verbatimLatitude: 58°22,30'N; verbatimLongitude: 10°20,33'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 10; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177107 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 351; maximumDepthInMeters: 387; verbatimLatitude: 58°22,19'N; verbatimLongitude: 10°23,50'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 10; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177108 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 180; maximumDepthInMeters: 216; verbatimLatitude: 58°28,16'N; verbatimLongitude: 10°37,04'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 11; habitat: Hard bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177110 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Kristineberg-Lysekil ; minimumDepthInMeters: 53; maximumDepthInMeters: 53; verbatimLatitude: 58°15,73'N; verbatimLongitude: 11°26,10'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177112 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskeb√§ckskil ; minimumDepthInMeters: 30; maximumDepthInMeters: 39; verbatimLatitude: 58°15,13'N; verbatimLongitude: 11°27,31'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2010; month: 8; day: 21; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177113 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 55; maximumDepthInMeters: 70; verbatimLatitude: 58°22,19'N; verbatimLongitude: 11°04,55'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 9; habitat: Mud and clay Type status: Other material. Occurrence: catalogNumber: SMNH-177114 ; individualCount: 1; sex: female; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Kristineberg-Lysekil ; minimumDepthInMeters: 53; maximumDepthInMeters: 53; verbatimLatitude: 58°15,73'N; verbatimLongitude: 11°26,10'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177117 ; individualCount: 2; sex: 1 female, 1 male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Klubban ; verbatimLatitude: 58°15,03'N; verbatimLongitude: 11°27,54'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Shells, gravel, sand, mud Type status: Other material. Occurrence: catalogNumber: SMNH-177118 ; individualCount: 2; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 44; maximumDepthInMeters: 44; verbatimLatitude: 58°15,63'N; verbatimLongitude: 11°27,72'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Soft mud Type status: Other material. Occurrence: catalogNumber: SMNH-177119 ; individualCount: 2; sex: female; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, Kristineberg-Lysekil ; minimumDepthInMeters: 53; maximumDepthInMeters: 53; verbatimLatitude: 58°15,73'N; verbatimLongitude: 11°26,10'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2014; month: 8; day: 14; habitat: Mud Type status: Other material. Occurrence: catalogNumber: SMNH-177122 ; individualCount: 1; sex: male; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 30; maximumDepthInMeters: 30; verbatimLatitude: 58°15,25'N; verbatimLongitude: 11°27,30'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Soft mud Type status: Other material. Occurrence: catalogNumber: SMNH-177123 ; individualCount: 2; sex: female; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 221; maximumDepthInMeters: 260; verbatimLatitude: 58°28,30'N; verbatimLongitude: 10°33,22'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 11; habitat: Soft bottom Type status: Other material. Occurrence: catalogNumber: SMNH-177096 ; individualCount: 3; sex: female; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 8; maximumDepthInMeters: 15; verbatimLatitude: 58°15,09'N; verbatimLongitude: 11°27,54'E; Identification: identifiedBy: O. Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Muddy sand Type status: Other material. Occurrence: catalogNumber: SMNH TYPE-9207 (same slide as C.triclados) ; individualCount: 3; sex: 1 female, 2 males; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 30; maximumDepthInMeters: 30; verbatimLatitude: 58°15,25'N; verbatimLongitude: 11°27,30'E; Identification: identifiedBy: Oleksandr Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Soft mud Type status: Other material. Occurrence: catalogNumber: SMNH TYPE-9206 (same slide as C.triclados) ; individualCount: 3; sex: 1 female, 2 males; Location: waterBody: Skagerrak; country: Sweden; locality: Gullmarsfjord, near Fiskebaeckskil ; minimumDepthInMeters: 30; maximumDepthInMeters: 30; verbatimLatitude: 58°15,25'N; verbatimLongitude: 11°27,30'E; Identification: identifiedBy: Oleksandr Holovachov; dateIdentified: 2018; Event: year: 2011; month: 8; day: 11; habitat: Soft mud Type status: Other material. Occurrence: catalogNumber: SMNH TYPE-9208 ; individualCount: 2; sex: 1 female, 1 male; Location: waterBody: Skagerrak; country: Sweden; locality: Bratten ; minimumDepthInMeters: 139; maximumDepthInMeters: 153; verbatimLatitude: 58°34,19'N; verbatimLongitude: 10°38,20'E; Identification: identifiedBy: Oleksandr Holovachov; dateIdentified: 2018; Event: year: 2012; month: 10; day: 12; habitat: Soft bottom

Description

Measurements. Table 6 . Adult. Figs 29 , 30, 31, 32. Cuticle without longitudinal striation. Space between dorsal and ventral limbs of amphid not developed. Lateral alae narrow, appearing externally as smooth uniform band with straight margins along most of body, but with distinct subcuticular channel and crenate margins when focused midway ( Fig. 32 a, b) along cloacal/anal region and anterior part of tail. It originates at posterior end of ventral limb of amphid and ends along posterior third of tail. Secretory-excretory pore opens apically on anterior end ( Fig. 31 b, c, d, e). Tail with clavate terminal part. Male. Anteriormost edge of amphid positioned at level with oral opening. Dorsal limb of amphid extends for a short distance posteriorly, equal to 2.7-3.5 labial region diameters in length. Ventral limb of amphid extends along pharyngeal region to level of anterior part of intestine; 4.6-5.3 times the length of dorsal limb. Ventral limb of amphid 1.3-1.7 wider than dorsal limb. Spicules with rounded manubrium and conoid, arcuate shaft. Gubernaculum platelike, without apophysis. One small precloacal papilliform sensillum located on 2nd annule anterior to cloacal opening ( Fig. 32 c, d). Second precloacal pore-like sensillum located on 7th-8th annule anterior to cloacal opening ( Fig. 32 c, d). Two pairs of ventrosublateral setae located along posterior half of tail and one pair of dorsosublateral setae located half way between posterior end of lateral alae and tail terminus. Female. Anteriormost edge of amphid positioned at level with oral opening. Dorsal limb of amphid extends for a short distance posteriorly, equal to 2.8-3.2 labial region diameters in length. Ventral limb of amphid extends along pharyngeal region just anterior to pharyngo-intestinal junction; 3.2-3.3 times the length of dorsal limb. Ventral limb of amphid 1.3-2.0 times wider than dorsal limb. Vagina straight ( Fig. 31 f). One pair of ventrosublateral setae located along middle of tail and one pair of sublateral setae located 1/3 way between posterior end of lateral alae and tail terminus.

Diagnosis

Body 0.53-0.63 mm long; cuticle without longitudinal striation; anteriormost edge of amphid at level with oral opening; dorsal limb of amphid equal to 2.7-3.5 labial region diameters in male, 2.8-3.2 labial region diameters in female; ventral limb of amphid extends towards anterior part of intestine in male and pharynx base in female, 4.6-5.3 times the length of dorsal limb in male and 3.2-3.3 times the length of dorsal limb in female; ventral limb of amphid is 1.3-2.0 times wider than dorsal limb; interamphideal space absent; secretory-excretory pore opens apically on anterior end; spicules 24-29 µm long; two precloacal supplements; tail equal to 4.5-6.4 anal body diameters in length, with clavate terminal part.

Taxon discussion

The original description of C. amphidialis is based only on three females, males not being known to the authors ( Fadeeva et al. 2016). Recent specimens are nearly identical to the type population in measurements and general morphology, including size and shape of the amphid. Furthermore, images of the type specimens kindly provided by Dr. N. Fadeeva, clearly show modified lateral alae along the caudal region in one of the specimens, confirming the conspecificity of both populations.

Figures

Figure 29. Campylaimus amphidialis Fadeeva, Mordukhovich & Zograf, 2016 (scale bars = 20 µm, PIJ = pharyngo-intestinal junction / cardia, EP = secretory-excretory pore): a: female tail; b: female pharyngeal region; c: male pharyngeal region; d: male caudal region.