Orphella helicospora Santam & Girbal, 1998.

Valle, Laia Guardia & Stoianova, Desislava, 2020, First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia, MycoKeys 67, pp. 55-80 : 55

Orphella helicospora Santam & Girbal, 1998. Figs 24 , 25

Specimens examined.

site 4: slide BUL-4-1; Site 7: slide BUL-7-6; site 10: slides BUL-10-2, BUL-10-5.

Notes.

Species were obtained from the hindgut lining of Leuctridae nymphs (mainly Leuctra hippopus ). We found several thalli, most of them producing trichospores and one also bearing helicoidal zygospores, formed homothallically, measuring 25-27 × 5.5-6.5 µm, growing on a fusiform zygosporophore measuring 20-23 × 7-8.5 µm, with a 3 µm long supporting cell and a sigmoid or reflexed intermediate cell about 20-24 µm long in the specimens seen (Fig. 24 ). All the characteristics of spores and accompanying cells fit those of the specimens reported from other localities in Europe, including Spain ( Santamaria and Girbal 1998; Valle and Santamaria 2005), Norway ( White and Lichtwardt 2004); Italy ( Valle et al. 2013). Thallus has the characteristic basal cell (Fig. 25 ) and lateral subsidiary branches.

Figures

Figures 21 - 25. Various species of Orphellales. 21 Orphella catalaunica from Leuctridae nymphs, trichospores and accompanying cells 22, 23 Orphella coronata from Nemouridae nymphs 22 allantoid trichospores and accompanying cells 23 zygospores produced homothallically 24, 25 Orphella helicospora from Leuctridae nymphs: 24 homothallical zygospores and accompanying cells 25 basal cell and holdfast. Scale bars: 25 μm in all figures.