Microgaster nixalebion Shaw, 2004

Hoecherl, Amelie, Shaw, Mark R., Boudreault, Caroline, Rabl, Dominik, Haszprunar, Gerhard, Raupach, Michael J., Schmidt, Stefan, Baranov, Viktor & Fernandez-Triana, Jose, 2024, Scratching the tip of the iceberg: integrative taxonomy reveals 30 new species records of Microgastrinae (Braconidae) parasitoid wasps for Germany, including new Holarctic distributions, ZooKeys 1188, pp. 305-386 : 305

Microgaster nixalebion Shaw, 2004

Material examined.

Austria: Lower Austria: Opponitz, ex. Patania ruralis , vi.2007, leg. J. Connell, MRS_JFT0934; Germany: Baden-Württemberg: Malsch, Hansjakobstr. 7, Urban Garden, 48.884, 8.32, 120 m, Malaise trap, 13.ix.2020, leg. D. Doczkal, ZSM-HYM-33153-H06; Malsch, Hardtwald NE Kieswerk Glaser, 48.915, 8.313, 125 m, Malaise trap, 9.vii.2011, leg. D. Doczkal, ZSM-HYM-42323-D11; Malsch, Luderbusch, 48.913, 8.332, 117 m, Malaise trap, 2.viii.2020, leg. D. Doczkal, K. Grabow, ZSM-HYM-42389-A08; 26.vii.2020, leg. D. Doczkal, K. Grabow, ZSM-HYM-42388-F12; Bavaria: Böbing, Ammertal, 47.747, 10.965, 686 m, 14.vii.2013, leg. D. Doczkal, ZSM-HYM-33420-D06; Dienhausen, 47.886, 10.827, 724 m, Malaise trap, 15.vii.2019, leg. J. Müller, ZSM-HYM-42325-C12; ZSM-HYM-42325-D01; ZSM-HYM-42380-A05; ZSM-HYM-42380-A06; ZSM-HYM-42380-A07; ZSM-HYM-42380-A08; Fabrikschleichach, Lichtung, 49.918, 10.56, 366 m, Malaise trap, 12.vii.2019, leg. J. Müller, ZSM-HYM-42382-F04; ZSM-HYM-42382-F05; Fabrikschleichach, close to Weilersbachtal, 49.917, 10.525, 408 m, Malaise trap, 12.vii.2019, leg. J. Müller, ZSM-HYM-42376-D10; ZSM-HYM-42376-D11; ZSM-HYM-42376-E01; Gütersleben, Gramschatzer Wald, 49.873, 9.932, 272 m, Malaise trap, 12.vii.2019, leg. J. Müller, ZSM-HYM-42379-F08; Lkr. Kelheim Abensberg-Sandharlanden, NSG Sandharlandener Heide, 48.845, 11.801, 376 m, Malaise trap, 3.viii.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-33157-F07; Lkr. Kelheim Siegenburg, Bombodrom, 48.755, 11.791, 411 m, Malaise trap, 8.ix.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-33168-H09; Lohr am Main, Beilstein, Weinberg Waldrand, 50.003, 9.563, 195 m, Malaise trap, 14.vii.2018, leg. D. Doczkal, ZSM-HYM-33156-A01; 3.vi.2018, leg. D. Doczkal, ZSM-HYM-33155-E07; Moos, Isarmündung, Molinia meadow, 48.779, 12.95, 313 m, Malaise trap, 30.vi.2021, leg. GBOL3, R. Albrecht, ZSM-HYM-42391-E06; Moos, Isarmündung, Stromtalwiese, 48.777, 12.994, 310 m, Malaise trap, 13.vii.2021, leg. GBOL3, R. Albrecht, ZSM-HYM-42395-E03; München, NSG Allacher Lohe, 48.199, 11.475, 502 m, Malaise trap, 19.viii.2021, leg. GBOL3, R. Albrecht, ZSM-HYM-42326-F04; 23.vi.2021, leg. GBOL3, R. Albrecht, ZSM-HYM-42326-B01; Berchtesgaden National Park, Königssee, Rinnkendlsteig, 47.551, 12.964, 695 m, Malaise trap, 9.viii.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-33162-B05; Berchtesgaden National Park, Wald west of St. Bartholomä, 47.547, 12.965, 620 m, Malaise trap, 28.vi.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-33156-D11; ZSM-HYM-33156-D12; ZSM-HYM-33156-E01; ZSM-HYM-33156-E02; Rhön Fladungen, NSG Schwarzes Moor, Kermi-Hochmoor, 50.512, 10.069, 780 m, Malaise trap, 9.viii.2017, leg. D. Doczkal, ZSM-HYM-33165-A02; Rhön Hausen, Kleines Moor, 50.487, 10.039, 890 m, Malaise trap, 25.vii.2018, leg. D. Doczkal, ZSM-HYM-33165-F06; Sankt Wolfgang, Wald, 48.466, 13.146, 474 m, Malaise trap, 13.vii.2019, leg. J. Müller, ZSM-HYM-42384-A03; Schärding, 48.436, 13.41, 304 m, Malaise trap, 13.vii.2019, leg. J. Müller, ZSM-HYM-42325-D06; ZSM-HYM-42325-D07; ZSM-HYM-42375-E09; Selb, Schönwald, 50.187, 12.1, 675 m, Malaise trap, 14.vii.2019, leg. J. Müller, ZSM-HYM-42381-F04; Siegenburg, Bombodrom, 48.755, 11.791, 411 m, Malaise trap, 13.vii.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-42324-D04; 23.viii.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-42324-G09; 29.vi.2017, leg. D. Doczkal, J. Voith, ZSM-HYM-42324-E12; Thiersheim, Karlmühle, 50.075, 12.152, 517 m, Malaise trap, 15.vii.2019, leg. J. Müller, ZSM-HYM-42381-A02; ZSM-HYM-42381-A05; Volkach, Kolitzheim, 49.922, 10.234, 229 m, Malaise trap, 16.vii.2019, leg. J. Müller, ZSM-HYM-42378-D02; Wimmelbach, close to Untere Mark, pond edge, 49.71, 10.994, 290 m, Malaise trap, 12.vii.2019, leg. J. Müller, ZSM-HYM-42381-C10; München, Obermenzing, Premises of Zoologische Staatssammlung, 48.1648, 11.4849, 519 m, Malaise trap, 31.vii.2017, leg. Axel Hausman, BIOUG42788-F01; Serbia: Southwestern Serbia, 2.3 km SE of Nova Varoš, along Creek, 43.443, 19.853, 1014 m, 14.vi.2009, leg. J. Skevington, CNCH1019; Spain: Can Liro; Barcelona, Catalonia, ex. Vanessa atalanta , 12.v.2006, leg. C. Stefanescu, WAM 0444; Catalonia, El Puig, ex. Vanessa cardui on Echium sp., 15.vi.2016, leg. C. Stefanescu, MRS_JFT0714; United Kingdom: England, Beetham, Cumbria, ex. Anthophila fabriciana , 15.viii.2013, leg. M. R. Shaw, MRS_JFT0342.

Geographical distribution.

PAL.

PAL- Austria*, Belgium, France, Germany*, Greece, Serbia*, Spain*, United Kingdom.

Molecular data.

BIN: BOLD:ABY6385.

Host information.

Choerutidae : type reared from Anthophila fabriciana (Linnaeus, 1767); also Prochoreutis myllerana (Fabricius, 1794); Nymphalidae : Aglais urticae (Linnaeus, 1758), Vanessa atalanta (Linnaeus, 1758); Vanessa cardui * (Linnaeus, 1758); Pyralidae : Patania ruralis (Scopoli, 1763).

Notes.

German specimens were identified by comparing with the information in the original description ( Shaw 2004) as well as specimens deposited in the CNC. Our sequences from German specimens are a 100% match for barcode sequences of several reared specimens: two specimens identified as M. nixalebion , one reared from Patania ruralis (MRS_JFT0934), one from Vanessa atalanta (WAM 0444=MRS_JFT 0029). There are two more reared specimens in this barcoding cluster, both males and initially determined only to genus, one from Vanessa cardui (Linnaeus, 1758) (MRS_JFT0714) and one from Anthophila fabriciana , the host of the holotype (MRS_JFT0342). A single specimen in this barcoding cluster differs by several mutations from the other sequences; until more specimens for morphological study are available we keep it as Microgaster sp. (ZSM-HYM-42325-G10) (see Fig. 34 ). This species is illustrated in Fig. 35 .

Figures

Figure 34. TCS haplotype network of BIN BOLD: ABY 6385, sequence length for analysis: 392 bp to accommodate MRS _ JFT 0342 = MARKB 109 - 21 ex. Anthophila fabriciana from the United Kingdom. The haplotypes morphologically identified as Microgaster nixalebion as part of this project are marked by a grey background. Each hatch mark in the network represents a single mutational change; small black dots at nodes indicate missing haplotypes. The diameter of the circles is proportional to the number of haplotypes sampled (see legend). The aligned sequences and traits can be reviewed in Suppl. materials 10, 11.

Figure 35. Microgaster nixalebion Shaw, 2004, female (ZSM-HYM- 42380 - A 06) A lateral and B dorsal views. Length of the specimen: 4.25 mm.