Tubeufia cocois X.G. Tian & Tibpromma et al. Journal of Fungi 8: 22 (2021).

Ma, Jian, Zhang, Li-Juan, Boonmee, Saranyaphat, Xiao, Xing-Juan, Liu, Ning-Guo, Xiao, Yuan-Pin, Luo, Zong-Long & Lu, Yong-Zhong, 2023, Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China, MycoKeys 99, pp. 87-108 : 87

Tubeufia cocois X.G. Tian & Tibpromma et al. Journal of Fungi 8: 22 (2021).

Fig. 5

Description.

Saprobic on submerged decaying wood in a freshwater stream. Sexual morph Undetermined. Asexual morph Hyphomycetous, helicosporous. Colonies on natural substrate superficial, effuse, gregarious, white. Mycelium superficial and partly immersed, hyaline, septate, branched hyphae, smooth, with glistening conidia. Conidiophores macronematous, mononematous, straight or slightly flexuous, cylindrical, unbranched, septate, 33-85 μm long, 5-7.5 μm wide (x̄ = 48.5 × 6 μm, n = 20), the lower part pale brown and the upper part hyaline, smooth-walled. Conidiogenous cells holoblastic, polyblastic, integrated, sympodial, terminal, cylindrical, denticulate, with a tooth-like protrusion, 1.5-4 μm long, 1.5-2.5 μm wide, truncate at apex after conidial secession, 4.5-10.5 μm long, 4.5-6 μm wide (x̄ = 8.5 × 5.5 μm, n = 20), hyaline, smooth-walled. Conidia solitary, acropleurogenous, helicoid, rounded at tip, 23-29 μm diam and conidial filament 4-6.5 μm wide (x̄ = 26 × 5.5 μm, n = 30), 100.5-138 μm long (x̄ = 118 μm, n = 25), indistinctly septate, coiled 2-21/2 times, not becoming loose in water, guttulate, hyaline, smooth-walled.

Culture characteristics.

Conidia germinating on water agar and producing germ tubes within 8 h. Colonies on PDA circular with flat surface and undulate edge. Growth rate 26 mm diameter in 35 days at 25 °C, with a dark brown to black surface.

Material examined.

China, Hainan Province, Qiongzhong Li and Miao Autonomous County, Baihualing Rainforest cultural tourism area, 18°98′N, 109°82′E, on rotting wood in a freshwater stream, 29 December 2021, Jian Ma, BH 5 (GZAAS 22-2038), living culture GZCC 22-2038 .

Notes.

Tian et al. (2022) introduced Tubeufia cocois from the dead leaves of Cocos nucifera in Thailand. Our newly isolated strain (GZCC 22-2038) clusters with T. cocois (MFLUCC 22-0001) with 93% ML/1.00 PP support. Morphologically, our new isolate shares the same morphological characteristics with the holotype (MFLU 21-0192) of T. cocois , thus we identified our new isolate as T. cocois . This is the first discovery of this species in a freshwater habitat and the first discovery of this species in China.

Figures

Figure 5. Tubeufia cocois (GZAAS 22 - 2038) a, b colonies on the host surface c-f conidiophores and conidiogenous cells g-j conidiogenous cells l-q conidia k germinated conidium r, s colonies on PDA, r from above s from below. Scale bars: 20 μm (c, d, f); 10 μm (e, g-q).