Stromatolinea guizhouensis K. Habib & Q. R. Li, 2024

Habib, Kamran, Zhou, Xin, Zeng, Wenyu, Zhang, Xu, Hu, Hongmin, Wu, Qianzhen, Liu, Lili, Lin, Yan, Shen, Xiangchun, Kang, Jichuan & Li, Qirui, 2024, Stromatolinea, a new diatrypaceous fungal genus (Ascomycota, Sordariomycetes, Xylariales, Diatrypaceae) from China, MycoKeys 108, pp. 197-225 : 197-225

Stromatolinea guizhouensis K. Habib & Q. R. Li sp. nov.

Fig. 3

Type.

• China, Guizhou Province, Anshun City , Pingba County (26 ° 15 ' 11 ″ N, 105 ° 56 ' 51 ″ E), altitude: 1,102 m, subtropical forest, on dead culms of Phyllostachys sp. , 25 August 2023, JWS- 28 (Holotype, GMB 4523 ; ex-type, GMBC 4523 ; isotype, KUN-HKAS 133214 ) .

Etymology.

The epithet “ guizhouensis ” refers to the locality of the collection, Guizhou province.

Description.

Saprobic on dead culms of Phyllostachys sp. , forming black parallel elongate ascostromata on the host. Pseudostromata absent. Sexual morph: Stromata 2–8.5 mm long, 350–800 μm wide, 400–600 μm high, parallel elongate, consistent in thickness, linear, long fusiform, solitary, sometime confluent, non-slit, distinctly gray at sides, often overlain by white crystalline, black at center, exposing black ostioles. Upper cells of stromata near the perithecial ostiole black, thick-walled. Stromatic tissue yellow between and beneath perithecia, compact. Ascomata 150–250 μm wide, 250–420 μm high, perithecial, frequent, 10–25 per stromata, immersed, linearly arranged, obpyriform, ostiolate centrally, with a neck, opening to outer surface, 70–90 × 35–60 μm, slight erumpent over stromata, appearing as black spots, slight shinny. Peridium 5–10 μm thick, cell elongate, texture angularis, outer thick layer dark brown, inner hyaline, surrounded by yellow stromatic tissue. Hamathecium paraphyses, filiform, hyaline, 54–70 × 1–3.2 μm. Asci 55–100 × 5.5–8 μm (x ̄ = 67.2 × 6.8 μm, n = 30), 8 - spored, unitunicate, clavate, with a long and thin pedicel, apically rounded to truncate, J- apical ring. Ascospores 5.8–9 × 1–2 μm (x ̄ = 7.6 × 1.5 μm, n = 30), allantoid, aseptate, straight to slightly curved, rounded at both ends, subhyaline, smooth-walled, single oil droplets in both ends. Asexual morph: Undetermined.

Culture characteristics.

Ascospores germinating on PDA within 24 hours, colonies on PDA, white when young, became pale, dense at centre, thinning toward the edge, reverse white at the margin, pale at the centre, no pigmentation, and no sporulation produced on the PDA medium.

Additional material examined.

• China, Guizhou Province, Huaxi District, Xiaohe Village , China (26 ° 29 ' 29 ″ N, 106 ° 42 ' 09 ″ E), altitude: 1,097 m, subtropical forest, on dead culms of Phyllostachys sp. , 2 January 2024, Xin Zhou & W. Y. Zeng, H- 8 ( GMB 4515 ) .

Notes.

Morphologically, Stromatolinea guizhouensis is similar to Stromatolinea linearis (= Diatrype phaselinoides Rappaz ; Eutypa linearis Rehm ), both exhibiting parallel elongate fusiform stromata with yellow stromatic tissue. However, it differs from S. linearis in having non-slit stromata, distinctly grey at sides, overlain by white crystalline material (Fig. 3 b, c ) and slightly larger ascospores (5.8–9 μm, x ̄ = 7.6 μm), compared to S. linearis with longitudinally slit stromata when mature and smaller ascospores (5–7 μm, x ̄ = 6.1 μm) ( Rehm 1907; Dai et al. 2017). From the other newly described species, it lacks pseudostromata and exhibits grey stromata overlain by white crystalline material.

Another morphologically similar species, Alloeutypa milinensis also features yellow stromatic tissue but can be easily differentiated by its stromata morphology. Alloeutypa milinensis exhibits scattered oblong to strip-shaped stromata measuring 0.9–2.2 mm in width and with larger ascospores (6.6–10.1 × 1.7–2.6 μm, x ̄ = 8.5 × 2.1 μm) ( Ma et al. 2023).

Figures

Figure 3. Stromatolinea guizhouensis (GMB 4523) a habitat of material b, c appearance of stromata on host d erumpent ostiole e horizontal section of ascostromata f vertical sections of ascomata in stroma g-i asci j apical ring of ascus k ascospores. Scale bars: 3 mm (b, c); 1 mm (d – f); 20 μm (g – j); 10 μm (k).