Sugiyamaella robnettiae C.Y. Chai & F.L. Hui, 2022

Chai, Chun-Yue, Gao, Wan-Li, Yan, Zhen-Li & Hui, Feng-Li, 2022, Four new species of Trichomonascaceae (Saccharomycetales, Saccharomycetes) from Central China, MycoKeys 90, pp. 1-18 : 1

Sugiyamaella robnettiae C.Y. Chai & F.L. Hui sp. nov.

Fig. 4

Etymology.

The specific epithet robnettiae named in honour of Christie J. Robnett for her proposal of the genus Sugiyamaella .

Type.

China, Henan Province, Luoyang City, Song County, the Tianchi Mountain National Forest Park , in rotting wood, October 2020, J.Z. Li & Z.T. Zhang (holotype NYNU 201066T, ex-type CBS 16663 = CICC 33513, holotype and ex-type are preserved in a metabolically inactive state) .

Description.

In YM broths after 3 days at 25 °C, the cells are ellipsoidal to elongate (2-4 × 2-8 μm) and occur singly or in pairs. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. On YM agar after 3 days at 25 °C, colonies are white to cream-coloured, convex, buttery and smooth with entire margins. In Dalmau plate culture on corn meal agar, pseudohyphae and true hyphae are formed. Asci or signs of conjugation are not observed on sporulation media. Fermentation of sugars is absent. Glucose, galactose, l-sorbose, glucosamine, d-xylose, l-arabinose, d-arabinose, l-rhamnose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, lactose, inulin, glycerol, erythritol, ribitol, xylitol, d-glucitol, d-mannitol, galactitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, 5-keto-d-gluconate, succinate, citrate, and ethanol are assimilated as sole carbon sources. d-ribose, melibiose, raffinose, melezitose, myo -inositol, d-gluconate, dl-lactate, and methanol are not assimilated. Nitrate, nitrite, creatine, glucosamine, and d-tryptophan are assimilated as sole nitrogen sources. Ethylamine, l-lysine, creatinine, and imidazole are not assimilated. Minimum growth temperature is 15 °C, and maximum growth temperature is 35 °C. Growth in the presence of 0.1% cycloheximide is present, but growth in the presence of 10% NaCl plus 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolates examined.

China, Henan Province, Luoyang City, Song County, the Tianchi Mountain National Forest Park, in rotting wood, October 2020, J.Z. Li & Z.T. Zhang (NYNU 201005).

Notes.

Two strains were collected from two different substrates, formed a well-supported group related to Su. chuxiongensis , representing a new species, Su. robnettiae . Su. robnettiae differs from Su. chuxiongensis by 1.9% substitutions in the D1/D2 domain and 6.4% substitutions in the ITS region. Physiologically, unlike Su. chuxiongensis ( Shi et al., 2021), Su. robnettiae is unable to assimilate d-ribose, melibiose, raffinose, or melezitose but is able to assimilate glycerol and lactose.

Figures

Figure 4. Morphology of Su. robnettiae (NYNU 201066, holotype) A budding cells were indicated by arrows in YM broth after 3 d B pseudohyphae and true hyphae on CM agar after 14 d. Scale bars: 10 μm.