Halodiatrype avicenniae Dayarathne & K.D.Hyde

Dayarathne, Monika C., Wanasinghe, Dhanushka N., Devadatha, B., Abeywickrama, Pranami, G, E. B., Jones, areth, Chomnunti, Putarak, Sarma, V. V., Hyde, Kevin D., Lumyong, Saisamorn, C., Eric H. & Mckenzie, 2020, Modern taxonomic approaches to identifying diatrypaceous fungi from marine habitats, with a novel genus Halocryptovalsa Dayarathne & K. D. Hyde, gen. nov., Cryptogamie, Mycologie 20 (3), pp. 21-67 : 54

Halodiatrype avicenniae Dayarathne & K.D.Hyde

( Fig. 15 )

Mycosphere 7 (5): 612-627 (2016).

CULTURE CHARACTERISTICS. — Colonies on PDA reaching 8 cm diam., after two weeks at 20-25°C, medium dense, irregular, slightly raised, surface smooth with undulate edge, cottony, colony from above white at the margin, yellowish white at the centre; from below yellowish white at the margin, yellow to pale brown at the centre; mycelium greenish grey, no pigmentation on PDA media. Conidiomata forming on culture media, concentrated at colony margin, appearing as black, slimy bubbles with conidial mass.

MATERIAL EXAMINED. — Thailand. Phetchaburi Province, Hat Chao Samran, 47°43’30.216”E, 40°15’1.368”N 0 m a.s.l., on intertidal decayed wood of Avicennia sp. at a mangrove stand, 28.VIII.2015, M. Dayarathne, CHAM020 (holo-, MFLU 16- 1185), ex-type living culture, MFLUCC15-0953, ICMP 21767; CHAM010 (MFLU 16-1176), ex-type living culture, MFLUCC 15-0948. — Krabi province, Tha pom Khlong Nam, 08°12’50.4”N, 98°46’42.7”E, 0 m a.s.l., on intertidal decayed wood of Avicennia sp. at a mangrove stand, 16.XII.2015, M. Dayarathne, KLA002 (MFLU 16- 1196), ex-type living culture, MFLUCC 16-0532; KLA003 (MFLU 16-1197), ex-type living culture, MFLUCC 16- 0533. — Ranong province, Amphoe Maung, Mu 4 Tambol Ngao, Ranong Mangrove Research Center, 9°43’- 9°57’N, 98°29’- 98°39’E, 0 m a.s.l., on intertidal decayed wood of Bruguiera cylindrica at a mangrove stand, 6.XII.2016, M. Dayarathne, MCD037 (MFLU 18-0150), ex-type living culture, MFLUCC 17-0396, ICMP 21765.

DISTRIBUTION. — Thailand.

DESCRIPTION

Sexual morph

See Dayarathne et al. (2016).

Asexual morph

(On PDA after 60 days at 25°C) libertella-like, in back conidial drops.

Conidia. Falcate, 26-38 × 1-2 µm (ẍ = 32 × 1.5 µm, n = 30), hyaline, 1-celled, smooth, upper end subacute, base truncate, curved to nearly semicircular.

NOTES

Halodiatrype avicenniae is morphologically distinguishable from H. salinicola by having aseptate, larger ascospores (45- 52 × 10-14 µm vs 7.5-8.5 × 2.5-3 µm), which are hyaline to light brown. Halodiatrype avicenniae is similar to H. mangrovei in its ascomata, asci, ascospore shape and colour ( Hyde 1993 ), but H. mangrovei has 1-4-septate ascospores, while they are aseptate in H. avicenniae . Unfortunately, there are no molecular data to compare the phylogenetic affinities of these two species. This study amends the species description by describing asexual morph characteristics of H. avicenniae . This study also reports Bruguiera cylindrica as a new host for this species.

Figures

FIG. 15. — B, C, F, H, I, K, M-P, R, S, Halodiatrype salinicola Dayarathne & K.D.Hyde (MFLU 15-0179 – holotype); A, D, E, G, J, L, O, P, T, U, Halodiatrype avicenniae Dayarathne & K.D.Hyde (MFLU 16-1185 – holotype and MFLU – 16-1197):A, B, horizontal section of ascoma (MFLU 16-1185,MFLU 15-0179);C, D, vertical section through ascoma (MFLU 15-0179, MFLU 16-1185); E, F, peridium (MFLU 16-1185, MFLU 15-0179); G, H, section through neck (MFLU 16-1185, MFLU 15-0179); I, J, asci (MFLU 15-0179 (in Congo red), MFLU 16-1185); K, L, paraphyses (MFLU 15-0179,MFLU 16-1185);M-P,ascospores (MFLU 15-0179,MFLU 16-1185);Q, conidia from culture on PDA (MFLU 18-0150);R-U,culture on PDA (MFLU 15-0179,MFLU 16-1185) Scale bars:A,1000 µm;B, 500 µm;C,D,I-L,100 μm;E, F,Q,20 μm;G-H,50 μm; M-P,10 μm