Cryptosphaeria eunomia (Fr.) Fuckel

Dayarathne, Monika C., Wanasinghe, Dhanushka N., Devadatha, B., Abeywickrama, Pranami, G, E. B., Jones, areth, Chomnunti, Putarak, Sarma, V. V., Hyde, Kevin D., Lumyong, Saisamorn, C., Eric H. & Mckenzie, 2020, Modern taxonomic approaches to identifying diatrypaceous fungi from marine habitats, with a novel genus Halocryptovalsa Dayarathne & K. D. Hyde, gen. nov., Cryptogamie, Mycologie 20 (3), pp. 21-67 : 38-40

Cryptosphaeria eunomia (Fr.) Fuckel

Jahrbücher des Nassauischen Vereins für Naturkunde 23-24: 212 (1870).

Sphaeria eunomia Fr. , Systema mycologicum (Lundae) 2 (2): 377 (1823).

DISTRIBUTION. — Denmark, Germany, Poland, Portugal, Russia, Sweden, Switzerland, Ukraine, as Cryptosphaeria millepunctata (synonym of Cryptosphaeria eunomia ), United States Marine based specimens from Pohnpei, Federated States of Micronesia western Pacific Ocean.

NOTES

Cryptosphaeria eunomia possesses a widely effused stroma within bark tissues that is slightly elevated, light colored or darkened, with separate, rounded or stellate ostioles at the surface. Asci are clavate or cylindrical, 8-spored, usually with long stalks, and paraphysate with allantoid, hyaline or brownish ascospores. It is widely known from Fraxinus spp. , Juglans regia , and Robinia pseudoacacia ( Rappaz 1987; Grand 1985; Dudka et al. 2004; Farr & Rossman 2018). It has also been isolated from an unidentified marine sponge from Pohnpei, and which yielded the anti-mycobacterial compounds diaporthein A and B ( Kim 2013). Unfortunately, we were unable to obtain herbarium material of Cryptosphaeria eunomia collected from a marine habitat and there are no sequences generated from marine based strains.

We used two strains of Cr. eunomia var. eunomia (CBS 216.87) and Cr. eunomia var. fraxini (CBS223.87) in our phylogenetic analyses.They grouped apart from all other Cryptosphaeria species and were located in a clade comprising Eutypa species ( Figs 1 ; 2 ).

Figures

FIG. 1. — Phylogram generated from the best scoring RAxML tree based on combined ITS and Btub sequence data. Bootstrap support values for maximum likelihood (ML,black) and maximum parsimony (MP, blue) equal or greater than 60 % are given above the nodes. Bayesian posterior probabilities (PP,green) equal or greater than 0.90 are shown above the branch. The tree is rooted to Xylaria hypoxylon (CBS 122620) and Kretzschmaria deusta (CBS 826.72). All sequences from ex-type strains are in bold. Symbols:, polysporous species;, octosporous species;, species with less than eight spores. Scale bar: 0.2 (expected number of nucleotide substitutions per site per branch).

FIG. 2. — Phylogram generated from the best scoring RAxML tree based on ITS sequence data. Bootstrap support values for maximum likelihood (ML, black) and maximum parsimony (MP, blue) equal or greater than 60% are given above the nodes. Bayesian posterior probabilities (PP,green) equal or greater than 0.90 are shown above the branch. The tree is rooted to Xylaria hypoxylon (CBS 122620) and Kretzschmaria deusta (CBS 826.72). Scale bar: 0.09 (expected number of nucleotide substitutions per site per branch).