Nocardioides silvaticus, Li & Shi & Zhang & Wang, 2019

Li, Chan, Shi, Kaixiang, Zhang, Yuxiao & Wang, Gejiao, 2019, Nocardioides silvaticus sp. nov., isolated from forest soil, International Journal of Systematic and Evolutionary Microbiology 69 (1), pp. 68-73 : 72-73

DESCRIPTION OF NOCARDIOIDES SILVATICUS SP. NOV.

Nocardioides silvaticus sp. nov. (sil.va′ ti.cus. L. masc. adj. silvaticus belonging to a forest).

Cells are Gram-stain-positive, strictly aerobic, rod-shaped, approximately 0.8–1.3 µm long and 0.3–0.4 µm wide, and non-motile. Colonies grown on R2A agar are creamcoloured, circular, smooth, raised and 1–2 mm in diameter after incubation for 3 days. The growth temperature and pH range on R2A agar are 20–42 Ǫ C (optimum, 37 Ǫ C) and pH 7–9 (optimum, pH 7). It can tolerate salt up to 5 % NaCl (optimum without addition of NaCl). Catalase is positive but oxidase is negative. Negative for H 2 S production, indole production, methyl red production and Voges–Proskauer test. Hydrolyses casein and DNA, but not gelatin, starch, cellulose, Tween 20, Tween 40, Tween 60 and Tween 80. As sole carbon sources, sucrose, fructose, cysteine, propionate, valerate, 3-hydroxybutyric acid, acetate, 3-hydroxy benzoate, L- serine, potassium gluconate and aesculin ferric citrate are utilized, but maltose, arabinose, glucose, celllobiose, rhamnose, sorbose, D- galactose, mannitol, sorbitol, D- ribose, seignette salt, L- proline, L- arginine, sarcosine, malic acid, sodium citrate and phenylalaline are not. For acid production, D- ribose, arabinose and xylose are positive, but glucose, cellobiose, rhamnose, sucrose, ribose, fructose, lactose, D-galactose, maltose, mannitol, sorbitol, sorbose and inositol are negative. Positive for alkaline phosphatase, esterase C4, esterase lipaes C8, valine arylamidase, leucine arylamidase, cysteine, trypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, b- glucosidase and a- glucosidase, but negative for lipase C14, a - chymotrypsin, a- galactosidase, b-galactosidase, b- glucuronidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase activities. The main respiratory quinone is meanquinone-8(H4). The major components of cellular fatty acids are iso-C, C 17:1! 8 c, C, C 17:1! 6 c and C 17: 0 10-methyl (TBSA). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid. The cellwall peptidoglycan contains LL- diaminopimelic acid.

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The type strain is S-34 T (= KCTC 49137 T = CCTCC AB 2018079 T), isolated from soil at Xinxiang Forest Ecology Park in Henan Province, PR China. The DNA G+C content of strain S-34 T was 71.2 %. The accession numbers of the 16S rRNA gene sequence and genome sequence of strain S-34 T are MH 341589 and QGDD 00000000, respectively .