Dematipyriforma muriformis D.F. Bao, K.D. Hyde & Z.L. Luo, 2022

Bao, Dan-Feng, Bhat, Darbhe J., Boonmee, Saranyaphat, Hyde, Kevin D., Luo, Zong-Long & Nalumpang, Sarunya, 2022, Lignicolous freshwater ascomycetes from Thailand: Introducing Dematipyriforma muriformis sp. nov., one new combination and two new records in Pleurotheciaceae, MycoKeys 93, pp. 57-79 : 57

Dematipyriforma muriformis D.F. Bao, K.D. Hyde & Z.L. Luo sp. nov.

Fig. 2

Etymology.

Referring to the muriform conidia of this species.

Holotype.

MFLU 21-0146.

Description.

Saprobic on submerged decaying wood. Sexual morph: Undetermined. Asexual morph: Colonies on substratum superficial, scattered, black, shining, granulate. Mycelium immersed, composed of hyaline, branched, septate, smooth, hyphae. Conidiomata sporodochial, subhyaline. Conidiophores 10-26.5 × 2-3 μm (x - = 18.2 × 2.3 μm, n = 20), micronematous to semi-macronematous, mononematous, fasciculate, simple or branched, hyaline, cylindrical, smooth. Conidiogenous cells monoblastic, integrated, terminal, determinate, hyaline, smooth. Conidia 23-26 × 15.5-18 μm (x - = 24.6 × 16.7 μm, n = 30), acrogenous, solitary, smooth, thick-walled, ellipsoidal to obovoid, muriform, rounded at apex, pointed at base, with 3-5 transverse septa, 1-longitudinal septum in all cells and rarely in end cells, slightly constricted at septa, subhyaline to pale olivaceous when young, olive to dark brown at maturity.

Material examined.

Thailand, Bangkok Province, Bang Kapi District , on decaying wood submerged in a freshwater stream, 3 October 2017, Z.L. Luo, Bsite 4-3-1 (MFLU 21-0146, holotype; KUN-HKAS 122858, isotype) .

Notes.

In the phylogenetic analysis, Dematipyriforma muriformis clustered with the ex-type strain of D. aquilaria (CGMCC 3.17268) within Pleurotheciaceae with low support (Fig. 1 ). The ITS blast result in NCBI GenBank showed that D. muriformis (MFLU 21-0146) is 92.36% and 91.92% similar to D. nigrospora (MFLUCC 21-0097) and D. aquilaria (CGMCC 3.17268) respectively.

Dematipyriforma muriformis resembles D. aquilaria in having micronematous, mononematous, smooth septate conidiophores, monoblastic, integrated, terminal, determinate conidiogenous cells and solitary, muriform conidia. However, D. muriformis differs from D. aquilaria in having hyaline conidiophores and slightly smaller conidia (23-26 × 15.5-18 vs. 25-37.5 × 15-22.5 μm). In addition, conidia of D. muriformis are subhyaline to pale olivaceous when young, olive to dark brown at maturity, with 3-5 transverse septa, 1-longitudinal septum in all cells and rarely in end cells. Whereas, D. aquilaria has pale grey olivaceous to pale brown conidia with 4-5 transverse septa and 0-2 longitudinal septa ( Sun et al. 2017).

Dematipyriforma muriformis shares some similar characteristics with Neomonodictys taxa in Pleurotheciaceae , such as monoblastic, integrated, terminal, determinate conidiogenous cells and muriform conidia. Neomonodictys , however, lacks sporodochial conidiomata and conidia of Neomonodictys are subglobose to globose, while, Dematipyriforma muriformis has ellipsoidal to obovoid conidia ( Hyde et al. 2020b).

Figures

Figure 1. Phylogram based on a combined ITS, LSU SSU, RPB 2 and TEF 1 - α sequence data of selected members of four orders of the Savoryellomycetidae. Bootstrap support values for maximum likelihood (ML) greater than 70 % and Bayesian posterior probabilities (PP) greater than 0.95 are given as ML / PP above the nodes. Newly obtained sequences are indicated in red and ex-type strains are in bold.

Figure 2. Dematipyriforma muriformis (MFLU 21 - 0146, holotype) a, b colonies on wood c-d conidiomata e-i conidiophore with conidia j-q conidia. Scale bars: 30 μm (c-d, o-q); 20 μm (e-n).