Mycetophila ruficollis Meigen, 1818

Juergenstein, Siiri, Kurina, Olavi & Poldmaa, Kadri, 2015, The Mycetophilaruficollis Meigen (Diptera, Mycetophilidae) group in Europe: elucidating species delimitation with COI and ITS 2 sequence data, ZooKeys 508, pp. 15-51 : 29

Taxon classification Animalia Diptera Mycetophilidae

Mycetophila ruficollis Meigen, 1818 Figs 10, 23, 24, 41, 42

Material.

POLAND. 1♂, Białowieża 2 km SW, Gródek, 52°41'02,8"N, 23°49'33,1"E, 17.viii.2007, sweeping, O. Kurina leg. (IZBE0200119, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ55). ESTONIA. 1♂, Tartu county, Maiorg near Annikoru, 58°16'41,6"N, 26°20'03,6"E, 17.ix.-2.x.2008, Malaise trap, O. Kurina leg. (IZBE0200120, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ229); 1♂, Jõgeva county, Pataste, 58°34'52,2"N, 26°46'42,3"E, 18. x– 30.x.2008, Malaise trap, J. Elts leg. (IZBE0200121, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ268); 1♂, Lääne county, Oonga, 58°0'41"N, 24°40'60"E, reared from Armillaria mellea , coll. 8.ix.1994, emerg. 21.ix.1994, O. Kurina leg. (IZBE0200123, pinned); 1♂, Saare county, Abruka, 58°9'50"N, 22°30'14"E, reared from Megacollybia platyphylla , coll. 11.ix.1991, emerg. 27.ix.1991, O. Kurina leg. (IZBE0200124, pinned); 1♂, Tartu county, Järvselja, 58°17'45"N, 27°15'41,7"E, reared from Pholiota aurivella , coll. 4.ix.1989, emerg. 25.ix.1989, O. Kurina leg. (IZBE0200125, pinned); 1♂, Tartu county, Järvselja, 58°17'45"N, 27°15'41,7"E, reared from Entoloma sp., coll. 27.viii.1989, emerg. 11.ix.1989, O. Kurina leg. (IZBE0200126, pinned); 1♂, Jõgeva county, Kõduküla, 58°34'N, 26°31'E, reared from Rhodocollybia butyracea , coll. 15.ix.2012, emerg. 2.x.2012, S. Jürgenstein leg. (IZBE0200127, in alcohol, abdomen used for DNA sequence: SJ339); 1♂, Saare county, Abruka, 58°9'50"N, 22°30'14"E, 21.ix.2013 reared from Armillaria borealis , coll. 21.ix.2013, emerg. 4.x.2013, O. Kurina leg. (IZBE0200128, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ382). GEORGIA. 1♂, Lagotekhi, 41°49'N, 46°17'E, 15. vi– 25.vi.2014, Malaise trap, G. Japoshvili leg. (IZBE0200247, in alcohol).

Male terminalia.

Posterior margin of gonocoxite undulate or slightly concave ventromedially, and with abrupt and blunt projections laterally. Posterior impression wide and uncompressed. Anterior impression with wide arms which are abruptly divergent anteriorly. Ventral branch of gonostylus with short and semicircular posterior process, with minute warts; ventral surface with long and slender bristles deviating from other setosity; spine 1 slender, sharply pointed, about half as wide as spine 2; spine 2 blunt, about half as high as spine 1; spines 3 and 4 smaller, sharply pointed, close to each other. Dorsal branch of gonostylus abruptly narrowed beyond the medial bristle. Posterior margin proximally from medial bristle with 7-9 gradually diminishing bristles; internal surface with a stronger seta next to the medial bristle. Lateral margin without concavity, almost straight. Basal angle slightly rounded, basal margin with few setae. Distal posterior process very shallow, almost unnoticeable; proximal posterior process high and massive, apically rounded. Distal posterior process with setae, proximal posterior process apically bare. Dorsal surface with a distinct band of setae from base of posterior processes to basal angle. Ejaculatory apodeme proximally narrows, with campanulate base and narrow rim. Aedeagal guides wide, apically widened and rounded, not extending beyond aedeagus distally; lateral impressions wide. Aedeagal apodemes laterally angular.

Intraspecific variation.

The combination of wide 4th and considerably short 5th palpal segments, the ventroapical margin of the gonocoxite without any medial projections and the apically abruptly narrowed dorsal branch of the gonostylus are unique among European species of the group.

Hosts and distribution.

Mycetophila ruficollis is reared from 35 species of macrofungi ( Yakovlev 1994). However, some of the rearing records in the literature may possibly refer to entire group: e.g. records from Lactarius and Russul a by Ribeiro (1990) and some records in Yakovlev (1994). Our records reveal saprotrophic members of the Agaricales as the host of this species. The species is widely distributed in Europe ( Chandler 2013, Jakovlev 2014, Kjærandsen 2012) extending also to the Middle East and Eastern Palaearctic ( Chandler 2013). The material from Georgia represents a new country record.