Entomobrya emiliae, Furgoł, Michał, 2017

Furgoł, Michał, 2017, Three new species of Entomobrya from Poland (Collembola, Entomobryidae), Zootaxa 4238 (1), pp. 43-57 : 44-47

Entomobrya emiliae sp. nov.

Figs 1–4 , 5–6 , 9 , 18–22 , 32–33 , Tables 1 , 2

Type material. Holotype, female on slide, 29 VI 2011. Poland, Brzeźno ad. Chełm , N 51°09’57”, E 23°39’15”, alkaline fens, ( Volhynian Polesia , the most western part of Polesia located in the East of Poland) . Paratypes: 7 specimens on slides and 16 specimens alc., same data as holotype. Deposited in the Department of Invertebrate Biology , Evolution and Conservation, University of Wrocław.

Additional material. 57 specimens alc. from Rozkosz ad. Chełm (25 VI 2009, N 51°08’37”, E 23°40’21”, alkaline fens, Volhynian Polesia , eastern Poland) ; 66 specimens alc. from Barwinek ad. Dukla (1 X 2009, N 49°26’46”, E 21°41’26”, meadow near a river, southeast Poland) ; 6 specimens alc. from Przemyśl–Winna Góra (2 X 2009, N 49°47’55”, E 22°46’16”, grassy and moist hill, southeast Poland) ; 49 specimens alc. from Jaksmanice ad. Przemyśl (2 X 2009, N 49°45’21”, E 22°53’27”, moist meadow, southeast Poland) ; 3 specimens on slides and 108 specimens alc. from Pszczeliny (28 IX 2010, N 49°09’43”, E 22°41’31”, tufts of grass in a moist meadow, Bieszczady Mountains , southeast Poland) ; 7 specimens on slides and 65 specimens alc. from Tarnawa Wyżna– Bukowiec (7 X 2011, N 49°04’09”, E 22°51’09”, moist area with tufts of grass along a tourist trail, Bieszczady Mountains , southeast Poland) ; 3 specimens on slides and 52 specimens alc. from Brzeźno (2 IX 2012, the same data as holotype) ; 13 specimens alc. from peripheries of Radom (26 V 2015, N 51°22’08”, E 21°06’30”, moist area with tufts of Carex sp. and Juncus sp., Central Poland) .

Description. Body length up to 3 mm excluding antennae. Body yellowish, usually with two longitudinal bands running from Th II and joining on posterior end of Abd IV, dark rectangle on Abd V and VI. Some individuals with subtle lines on both sides of head and on lateral sides of Th II and III ( Figs 5 , 32 ). Some specimens without colour pattern ( Figs 6 , 33 ).

Head. Eight ocelli, GH smaller than EF. Antennae length 2352 µm (holotype), more than 3 times head's length, Ant IV with bilobed apical vesicle. Relative length of Ant I/II/III/IV = 1/1.2/?/2; in paratypes = 1/1,2–1,4/1,1–1,3/ 1,9–2,3. Labral papillae smooth and more or less conical ( Fig. 19 ).

Body. Length ratio of Abd IV/III>4. Trochanteral organ as in Fig. 18 . Claw with 4 inner teeth: paired at 50% and proximal unpaired at 78% from base (holotype), with distal unpaired minute; dorsal tooth more or less on internal teeth level. Unguiculus lanceolate, with smooth external edge on leg III. Tenent hair as in Fig. 20 . Length of manubrium + dentes: 780 + 900 µm (holotype). Manubrial plate with over 10 chaetae and 2 pseudopores ( Fig. 22 ). Mucronal subapical tooth similar in size to apical one, mucronal spine present ( Fig. 21 ). Measurements of selected body parts are listed in table 1.

Chaetotaxy. Simplified basic formula: 3-2(+ 1m)-0-3-2(+ 1m)/3-7/2-4(5)/0-2-1/4(3-6)-1 0 4-1-1(2)-3(4); Head chaetotaxy as in Fig 1 . Thorax chaetotaxy ( Fig. 2 ): Th II area T1 with 3 Mc (m 1, m 2 and m 2i), area T2 with 7 Mc (a 5, m 4, m 4i, m 4p, m 4pi, m 4pi2 and m 5). Abdomen chaetotaxy ( Figs 3–4 ): Abd II area A1 with 2 Mc (a 2 and a 3), area A2 with 5 Mc (m 3, m 3e, m 3ea, m 3ei and m 3ep) (in larger specimens (also in holotype) there are 5 macrochaetae in A2 area, while in smaller specimens (but already adult) there are often 4 macrochaetae in the same area). Abd III area A3 without Mc, area A4 with 2 Mc (a 2 and a 3) and area A5 with 1 Mc (m 3). Abd IV plurichaetotic; sometimes with asymmetric distribution of supplementary Mc or m in areas A6, A7 and A8, especially in young adult specimens (in some young adult specimens there is one additional m or Mc in A04 on the A8 area of Abd IV).

Biology. Swampy and moist habitats. The species was usually recorded on grasses and sedges ( Carex sp., Juncus sp. and Phragmites sp.).

Discussion. The main colour form of this species resembles that of E. dorsolineata Jordana, Schulz & Baquero, 2011 . Concerning chaetotaxy, fourteen Palaearctic species have 0-2-1 Mc in areas A3, A4 and A5 of Abd III, but only four have 2-4 Mc in areas A1 and A2 on Abd II ( E. karlstetterae Baquero & Jordana, 2008 , E. koreana Yosii, 1965 , E. monopunctata Lee & Park, 1984 , E. oleniensis Tshelnokov, 1987 ) and there is one species which has the overlapping formula of chaetotaxy 1(2)-3(4) on Abd II ( E. barogii Baquero & Jordana, 2015 in: Baquero et al. 2015). However, they differ from the new species in colouration of body and chaetotaxy of Abd IV.

The two colour forms of E. emiliae differ in presence/absence of two longitudinal lines on dorsal side of a body ( Figs 5 , 32 vs. 6, 33). This feature is not connected with springtail's sex. Striped form predominates while nonstriped form was found almost exclusively at the type locality. I presume that longitudinal lines may disappear in older specimens.

Etymology. This species is dedicated to my wife Emilia.

Figures

FIGURE 9. Records of E. emiliae sp. nov. (triangles), E. flavovirens sp. nov. (circles) and E. kasprowiensis sp. nov. (squares).

FIGURES 1 – 4. E. emiliae sp. nov.: 1, Dorsal head macrochaetotaxy; 2, Dorsal Th II macrochaetotaxy; 3, Dorsal Abd II – III macrochaetotaxy; 4, Dorsal Abd IV macrochaetotaxy.

FIGURES 5 – 8. Colour patterns of new species (only two first segments of antennae shown): 5 – 6, Two forms of E. emiliae sp. nov.; 7, E. flavovirens sp. nov.; 8, E. kasprowiensis sp. nov.

FIGURES 18 – 31. Morphological characters of three new species of Entomobrya. E. emiliae sp. nov. 18 – 22: 18, Trochanteral organ; 19, Labral papillae; 20, Tip of tibiotarsus and claw of leg III; 21, Tip of dens and mucro; 22, Manubrial plate. E. flavovirens sp. nov. 27 – 31: 27, Trochanteral organ; 28, Labral papillae; 29, Tip of tibiotarsus and claw of leg III; 30, Ant IV apical vesicle; 31, Manubrial plate. E. kasprowiensis sp. nov. 23 – 26: 23, Labral papillae; 24, Trochanteral organ; 25, Tip of tibiotarsus and claw of leg III; 26, Manubrial plate.

FIGURES 32 – 33. The appearance of two colour forms of E. emiliae sp. nov. in culture.

Tables

TABLE 1. Measurements of the studied specimens (holotypes), in micrometres, ‘ - ‘: no data.

	E. emiliae n. sp.	E. flavovirens n. sp.	E. kasprowiensis n. sp.
Ant I	565	150	80
Ant II	650	247	155
Ant III	-	236	155
Ant IV	1137	306	205
Antennae	2352	939	595
Head	617	305	300
Ant/head ratio	3,81	3,08	1,98
Th II	407	170	137
Th III	237	125	77
Abd I	187	100	57
Abd II	235	120	92
Abd III	142	127	102
Abd IV	912	477	357
Abd IV/III ratio	6,42	3,75	3,5
Abd V	170	110	92
Abd VI	105	50	65
Body	3012	1584	1279
Manubrium	780	385	262
Dens	900	412	310
Claw	50	34	25
Unguiculus	36	22	18
Tenent hair	70	46	33

TABLE 2 Diagnostic characters of Entomobrya species most similar to E. emiliae sp. nov. in terms of colouration (E. dorsolineata) and macrochaetotaxy (E. barogii, E. karlstetterae, E. koreana, E. monopunctata, E. oleniensis). U, no data; *, Number of differences between each species and the new one.

Character/Species H1 area (head): Mc number An2-An3	dorsolineata . E 4*	barogii . E 4-6*	karlstetterae . E 5*	koreana . E U	monopunctata . E U	oleniensis . E 3	. sp emiliae .. nov E 3
H2 area (head): Mc number A5-A7	2	1*	1*	U	U	1*	2(+1m)
H3 area (head): Mc number S'0	0	1*	1*	U	U	0	0
H4 area (head): Mc number S1-S3-S4	3*	2	2	U	U	2	2
H5 area (head): Mc number Ps2-Ps3-Ps5	1b*	2	3*	U	U	2	2(+1m)
Labral papilla: 1, without/ 2, simple and smooth papilla/ 3, multispinose or with some projections/ 4, a projection chaetae like	3*	2	3*	4*	4*	4*	2
Eyes size: 1, GH=EF; 2, GH<EF	2	2	1*	2	2	2	2
Ant. vesicle: 1, no bulb/ 2, lobule simple/ 3, bilobed/ 4, trilobed	2*	2*	3	2*	2*	2/3	3
Ant./head ratio: 1,> or = 3/ 2,> or = 2 <3/ 3, <2	1	2*	2*	1	2*	2*	1
T1 area (Th II): Mc number m1-m2i2	2*	0*	0*	4*	U	4*	3
T2 area (Th II): Mc number a5-m5	4*	4*	2*	4*	U	5*	7
Tibiotarsi, smooth setae: 1, not present or only 1 on tibiotarsus III/ 2, double file on all tibiotarsi;	1	1	1	1	1	1	1
Claw, dorsal tooth: 1, absent/ 2, basal/ 3, internal teeth level/ 4, intermediate	2*	4*	U	U	2*	3	3
Unguiculus, externally: 1, smooth/ 2, serrate/ 3, with tooth	2*	1	1	1	1	2*	1
A1 area (Abd II): Mc number a2-a3	2	1-2	2	2	2	2	2
A2 area (Abd II): Mc number m3	5	3-4	4	4	4	4	4(5)
A3 area (Abd III): Mc number a1	1*	0	0	0	0	0	0
A4 (Abd III): Mc number m2	2	2	2	2	2	2	2
A5 (Abd III): Mc number m3-m4	1	1-2	1	1	1	1	1
A6 (Abd IV): Mc number A1-E1 (9 if> 8)	0*	0*	0*	0*	0*	0*	4(3-6)
A7 (Abd IV): Mc number A2-E1 (10 if> 9)	1 0 7*	0*	4*	4*	2*	1*	1 0 4
A8 (Abd IV): Mc number A4-C2a (6 if> 5)	0*	3-4*	3*	4*	1	3*	1
A9 (Abd IV): Mc number A5-B5 (6 if> 5)	3*	2	2	3*	2	2	1(2)
A10 (Abd IV): Mc number A6-B6 (6 if> 5)	3	2*	2*	2*	2*	2*	3(4)
Abd IV/Abd III ratio: 1, 2 <R <4/ 2, R> 4	2	1*	2	2	2	2	2
Manubrial plate chaetae number (11 if> 10)	4*	5*	8*	U	U	4*	11
Mucro, sub-apical tooth: 1, without/ 2, normal/ 3, bigger than apical/ 4, smaller than apical	3*	2	2	2	3*	2	2
Sum of the differences between the new species and the other species.	16	14	14	9	8	11