Wohlfahrtia magnifica (Schiner, 1862)

Szpila, K., Hall, M. J. R., Wardhana, A. H. & Pape, T., 2014, Morphology of the first instar larva of obligatory traumatic myiasis agents (Diptera: Calliphoridae, Sarcophagidae), Parasitology Research (1629) 113 (5), pp. 1629-1640 : 1633

Wohlfahrtia magnifica (Schiner, 1862)

( Figs. 4a–h ; 5a–e ; 6e, f ; and 7c )

Pseudocephalon: Antennal complex with slightly conical dome, height of basal ring greater than length of antennal dome ( Fig. 4c ); maxillary palpus encircled by several cuticular folds, central cluster of sensilla with three sensilla coeloconica and three sensilla basiconica (sb1–sb3, sc1–sc3) ( Fig. 4d ), few other small sensilla are situated close to sb1, two additional sensilla coeloconica (ns1–ns2) of typical appearance arranged laterodorsally on the surface of the maxillary palpus ( Fig. 4d ); ventral organ small, situated lateral to the functional mouth opening and level with the adjacent integument ( Fig. 4b, e ); oral ridges terminate medio-laterally on pseudocephalon ( Fig. 4a ). Cephaloskeleton: mouthhooks large and strongly sclerotised, anterior part of each mouthhook strongly curved downward and with single pointed tip, basal part with well visible lateral arm, tips of teeth orientated ventrally ( Figs. 4a, b and 6e ); labrum very large and long with massive basal part, anterior part of labrum strongly curved downward appearing to represent a third, middle mouthhook ( Figs. 4a, b and 6e ); intermediate sclerite short, partly hidden behind parastomal bar in lateral view but clearly shifted toward anterior end of body under base of labrum ( Fig. 6e, f ); parastomal bars short and broad ( Fig. 6e, f ); vertical plate wide, about three times wider than width of ventral cornua; dorsal cornua longer than ventral cornua, but both cornua of similar width ( Fig. 6e ); dorsal bridge present. Thoracic segments: anterior spinose band on t1 broad, with spines arranged in 5–6 rows dorsally and 9–11 rows ventrally, spines very large more conical than in Chrysomya bezziana , elongated and slightly curved, size of spines decreasing gradually towards the posterior end of body ( Fig. 4a, b ); anterior spinose bands of t2 and t3 with homogeneous, strongly sclerotised, elongated spines. Abdominal segments: anterior spinose bands complete on a1–a5, on a6 band narrowly interrupted dorsally, on a7 the band incomplete, restricted to ventral surface and few spines on dorso-lateral surfaces, each anterior spinose band ventrally with a transverse lenticular gap without spines ( Figs. 5b and 7c ); posterior spinose band on a1– a6 band present as a single row of ventral spines with few additional spines ventro-laterally, band on a7 complete with a single row of spines on lateral surfaces and with 2–3 rows ventrally and dorsally; lateral creeping welts with strong spines directed posteriorly, only the most posterior lateral creeping welt without spines. Anal division: Anal pads rounded, small and slightly protruding ( Fig. 5e ), anal tuft with several spines dorsally, readily apparent in light microscope; hair-like spines around spiracular cavity present but sparse ( Fig. 5d ); posterior spiracles hidden in spiracular cavity ( Fig. 5d ); p1, p3 and p5 developed as large cones with a long sensillum on the extremity resembling a large sensillum coeloconicum ( Fig. 5c, d ), p7 with sensillum on small protuberance, p2, p4 and p6 developed as sensilla situated level with adjacent integument.

Figures

Fig. 4 First instar of Wohlfahrtia magnifica. a Anterior end of body, lateral view. b Anterior end of body, ventral view. c Antennal complex. d Maxillary palpus. e Ventral organ. f Keilin’s organ. g Third thoracic segment, spines. h Second abdominal segment, spines. Abbreviations: as anterior spiracle, lb labrum, mh mouthhooks

Fig. 5 First instar of Wohlfahrtia magnifica. a Third abdominal segment, dorsal view. b Third abdominal segment, ventral view. c Anal division, papilla p5. d Anal division, posterior end, dorsal view. e Anal division, posterior end, ventral view

Fig. 6 Cephaloskeleton of OTMA. a Chrysomya bezziana, lateral view. b Chrysomya bezziana, ventral view. c Cochliomyia hominivorax, lateral view. d Cochliomyia hominivorax, ventral view. e Wohlfahrtia magnifica, lateral view. f Wohlfahrtia magnifica, ventral view. Scale bar=0.1 mm. Abbreviations: a length of apical part of labrum, b length of basal part of labrum, db dorsal bridge, dc dorsal cornua, is intermediate sclerite, la lateral arm, lb labrum, mh mouthhook, pb parastomal bar, vc ventral cornua, vp vertical plate

Fig. 7 Habitus. a Chrysomya bezziana. b Cochliomyia hominivorax. c Wohlfahrtia magnifica. d Lucilia cuprina. Abbreviations: a1–a7 abdominal segments 1–7, ad anal division, pc pseudocephalon, t1–t3 thoracic segments 1–3. Scale bar=0.1 mm