Radomaniola gracilipenia Szarowska et Falniowski, 2025

Jaszczyńska, Aleksandra, Grego, Jozef, Beran, Luboš, Hofman, Sebastian, Falniowski, Andrzej & Osikowski, Artur, 2025, Five new species of Radomaniola Szarowska, 2006 (Truncatelloidea: Hydrobiidae), Zootaxa 5716 (2), pp. 207-233 : 223-224

Radomaniola gracilipenia Szarowska et Falniowski sp. nov.

Zoobank number: urn:lsid:zoobank.org:act:

GenBank sequence numbers: KC011744

mOTU F

Type locality GREECE • North Peloponnísos Peninsula, Achaia, Province Kalavryta, spring at Bouboukas, near road to Maneli (locality 24: Figs 1 and 2C ) ; 38°00’53”N; 21°58’31”E; 703 m a.s.l.; on stones and leaves in the spring head.

Holotype GREECE • ethanol-fixed specimen ( Fig. 12A ); spring at Bouboukas, near road to Maneli, North Peloponnísos Peninsula , Achaia , Province Kalavryta , Greece (locality 24: Figs 1 and 2C ); 38°00’53”N; 21°58’31”E; 703 m a.s.l.; 12.09.2007; Magdalena Szarowska and Andrzej Falniowski legs.; NHMW-MO-113908.

Paratypes GREECE •18ethanol-fixed specimens from type locality;16 ethanol-fixed specimens ZMUJ/2024 /04; 2 ethanol-fixed specimens NHMW-MO-113909 .

Description Shell ( Fig. 12A–D ) up to 2.16 mm high, ovate-conical, broad, spire moderately low, about 5½ whorls, spire height 26–29 of the shell height. Teleoconch whorls slightly convex, evenly rounded, growing regularly and slowly in diameter. Aperture moderately broad and ovoid, outer lip simple, parietal lip complete, moderately broad or broad, umbilicus in the form of long slit. Teleoconch moderately thick-walled, its wall translucent, growth lines delicate. Periostracum yellowish. Shell measurements: Table 3, Fig. 15 . Operculum yellowish, smooth on both surfaces.

Soft parts morphology and anatomy Head and mantle intensely pigmented black. Female reproductive organs ( Fig. 12I ) with large, bulky, spherical bursa copulatrix with short duct, moderately thick loop of oviduct. Proximal seminal receptacle (rs 2 of Radoman 1983) elongated, finger-shaped, distal one (rs 1 of Radoman 1983) big, elongated. Penis ( Fig. 12E–H ) enormously large (about 900 µm), slender, ribbon-shaped, sharply terminated, more or less broadened at its base. At the middle part of the penis, of its left side, extremely minute single outgrowth. Vas deferens running in zigzag and is well visible inside the penis.

Differential diagnosis From the most closely related species R. filiola it differs in having a larger, longer and smooth penis with either absent or vestigial outgrowth. The Molecular Diagnostic Characters in comparison with other new Radomaniola species: binary: 39(A), 63(T), 75(T), 76(A), 138(G), 147(C), 171(C), 213(A), 235(T), 411(G), 426(T), asymmetric: 57(G), 249(C), 390(G). In comparison with R. filiola : binary: 39(G), 93(G), 138(G), 235(T).

Etymology The name reflects slender and long penis with vestigial outgrowth.

Distribution and habitat Known so far only from its type locality.

Ecological status The species is known from a single locality at Bouboukas at Northwestern Peloponnese Peninsula. The AOO and EOO are represented by a very small 5 m 2 spring zone already altered by the construction of the fountain. Locality is strongly dependent upon a small supply of water and is threatened by climatic changes and drought.Additionally, locality is endangered by anthropogenic pollution by municipal or chemical waste (detergents) from visitors of the fountain, as well as by further anthropogenic alterations of the fountain by construction activities or tap water catchments. Therefore, it is assessed as Endangered (En).

Figures

FIGURE 1. Studied localities. Colours indicate mOTUs as in Fig. 3. Two-color dots indicate localities where individuals belonging to two mOTUs were found.

FIGURE 2. Some of the studied localities.A—spring of the river Cetina (Milaševo jezero), Croatia, locality 3; B—river Cetina near Obrovac Sinjski, Croatia, locality 10; C—spring at Bouboukas, near road to Maneli, Peloponnisos, Greece, locality 24, type locality of Radomaniola gracilipenia sp. nov.; D - spring Borak, Vrelo Lištice in Široki Brijeg, Bosnia and Herzegovina, locality 16, (photo Mario Olšavský); E and H—spring Mislina on bank of Jezero Kutac, Croatia, locality 14, type locality of Radomaniola mislinensis sp. nov. (photo Roman Ozimec); F—spring Vrioštica in Vitina, Bosnia and Herzegovina, locality 17, type locality of Radomaniola testavariabilis sp. nov. (photo Mario Olšavský); G—Vrelo Kosinac 1 in Obrovac Sinjski, Croatia, locality 15.

FIGURE 12. Radomaniola gracilipenia sp. nov., type locality (locality 24): A–D—shells: A—holotype, B—sectioned, C—sectioned, D—sectioned, bar equals 1 mm; E–H—penes, bar equals 200 µm; I—female reproductive organs. (bc—bursa copulatrix, cbc—duct of bursa copulatrix, ov—oviduct, ovl—loop of (renal) oviduct, rs1, rs2—seminal receptacle, terminology after Radoman (1973): rs1—distal, rs2—proximal).

FIGURE 15. Principal component analysis of the seven morphometric shell variables. PC1 explains 54%, PC2 36% and PC3 6% (cumulatively 96%) of the total variance.