Coprinellus campanulatus Hussain & Ahmad

Hussain, Shah, Usman, Muhammad, Afshan, Najam-ul-Sehar, Ahmad, Habib, Khan, Junaid & Khalid, Abdul Nasir, 2018, The genus Coprinellus (Basidiomycota; Agaricales) in Pakistan with the description of four new species, MycoKeys 39, pp. 41-61 : 43-44

Coprinellus campanulatus Hussain & Ahmad sp. nov. Figures 1Eand 4

Diagnosis.

The diagnostic features of Coprinellus campanulatus are: campanulate pileus with greyish-olive tinge, surface with glistening clusters of micaceous veil at maturity, dark yellowish-brown centre, basidiospores 8.0-10.5 × 5.5-6.5 × 4.5-5.5 µm, spores mitriform in face view and cylindrical to amygdaliform in side view.

Type.

PAKISTAN: Khyber Pakhtunkhwa, Qaldara, Dargai, Malakand, 480 m alt., gregarious on wood chip, 14 Aug 2014, S. Hussain, SH144 (LAH-SH-144, holotype); GenBank accession ITS: MH753667.

Etymology.

The epithet “campanulatus” (Latin) refers to the campanulate shape of the pileus of this species.

Macroscopic characters.

Pileus at young stage 3-8 × 3-7 mm, ovoid to parabolic, light orange-yellow (7.5YR 9/8) to pale orange-yellow (7.5YR 9/4), surface pruinose; at mature stage 25-40 × 10-15 mm, pulvinate to campanulate, light greyish-olive (10Y 5/2) to greyish-olive (5Y 3/2), centre slightly campanulate, strong yellowish-brown (10YR 4/8) to dark yellowish-brown (10YR 1/2); surface finely furfuraceous to granulose, with clusters of micaceous-glistening veil, bright white, plicate from near centre to margin; context membranous to submembranous. Lamellae adnexed, narrow, with fimbriate edge, crowded with 1-4 series of lamellulae, pale orange-yellow (7.5YR 9/4) at young stage, dark yellowish-brown at maturity (10YR 2/2). Stipe 70-100 × 3-7 mm, equal, white, surface smooth, context hollow. Annulus absent with a membranous layer at the base. Odour pungent. Not tasted.

Microscopic characters.

Basidiospores (7.0 –)8.0–10.5(– 11.5) × (5.0 –)5.5–6.5(– 7.0) × (4.0 –)4.5–5.5(– 6.0) µm, on average 9.4 × 5.7 × 5.1 µm, Q1 = 1.6, Q2 = 1.8, av. Q = 1.7; in face view mitrifrom, triangular to ellipsoid; in side view cylindrical, amygdaliform to ellipsoid; dark brown to blackish in KOH, smooth, thick-walled, with truncate base, apiculus visible, germ-pore 1.5-2.5 µm wide, central, prominent, pale to hyaline. Basidia 19-29 × 7-10 µm, cylindrical, clavate to subclavate, hyaline, 4-spored. Cheilocystidia 36-47 × 35-45 µm, globose to subglobose, hyaline, abundant. Pleurocystidia absent. Pileipellis an epithelium of loosely arranged globose to subglobose or ellipsoid, hyaline to light olive, thin-walled elements, 30-80 × 25-60 µm. Veil composed of globose to subglobose cells, 50-90 µm diam., slightly thick-walled, yellowish-brown in KOH. Caulocystidia absent. Clamp connections rarely present.

Habitat and distribution.

Gregarious on woody litter under Morus alba , so far only known from lowland northern Pakistan.

Additional specimens examined.

PAKISTAN: Khyber Pakhtunkhwa, Malakand, Qaldara, on woody pasture, 14 August 2014, S. Hussain, SH144 (SWAT SHP144).

Comments.

The main distinguishing features of Coprinellus campanulatus are: campanulate pileus with greyish-olive tinge, dark yellowish-brown centre, veil on pileus in the form of micaceous-glistening clusters which are composed of globose to subglobose cells and basidiospores 8.0-10.5 × 5.5-6.5 × 4.5-5.5 µm, spores mitriform in face view and cylindrical to amygdaliform in side view. Based on veil anatomy, Co. campanulatus belongs in sect. Micacei . Coprinellus micaceus and Co. truncorum are most closely related to Co. campanulatus amongst the species sampled for our phylogenetic analyses. The new species Co. campanulatus with pulvinate to campanulate pileus can be differentiated from Co. micaceus and Co. truncorum , which have broadly convex pilei. At maturity, the pileus is light brown in Co. micaceus and Co. truncorum when compared to Co. campanulatus with greyish-olive pileus. On basis of spore morphology, Co. campanulatus can be differentiated from Co. micaceus . Basidiospores in Co. micaceus are slightly smaller (6.5-10.0 × 4.5-7 µm), lacrimiform to submitriform or mitriform in face view, conical towards base ( Keirle et al. 2004, Uljé 2005). In Co. micaceus , voluminous, broadly clavate, (sub)globose to ellipsoid pleurocystidia up to 150 × 70 µm are present, in Co. campanulatus pleurocystidia are absent. Also, in C. micaceus , caulocystidia are abundant, in Co. campanulatus absent. Spores of Co. truncorum are 8.5-9.0 × 5.5-6 µm, ellipsoid in all views, not distinctly lentiform, with very broad central to slightly eccentric germ pore, broadly rounded apex, not truncate, smooth, dark grey to grey brown or black ( Keirle et al. 2004, Uljé 2005).