Mucor nederlandicus Vanova , Ceska Mykol. 45(3): 128 (1991)

Hurdeal, Vedprakash G., Gentekaki, Eleni, Hyde, Kevin D., Nguyen, Thuong T. T. & Lee, Hyang Burm, 2021, Novel Mucor species (Mucoromycetes, Mucoraceae) from northern Thailand, MycoKeys 84, pp. 57-78 : 57

Mucor nederlandicus Vanova, Ceska Mykol. 45(3): 128 (1991)

Figs 1 , 4

Gene sequences.

(ITS) MZ433254; (LSU) MZ433251

Material examined.

Thailand, Chiang Rai Province, Muang District , Mae Salong Nai , 20°12'38.0"N; 99°37'55.6"E, from soil, 10th June 2020, collected by Bhavesh Raghoonundon, and isolated by Vedprakash Godadhar Hurdeal, living culture, MFLUCC 21-0045.

Description.

Asexual morph (based on cultures grown in MEA at 25 °C): Sporangiophores hyaline, up to 7.5 µm (x - = 5, n = 30) in diameter, undulate, occasionally curved, irregular septate near the base (1-2), unbranched, sympodial branches formed. Sporangia 20-47.5 × 20-45.5 µm (x - = 34 × 35.5 µm, n = 28), globose, smooth-walled, thick-walled, wall echinulate, deliquescent in mature sporangia, hyaline to pale brown. Columellae 12.5-23.5 × 12-22.5 µm (( x - = 16 × 15.5 µm, n = 30), mostly globose to subglobose, and sometimes oblong, obovoid, and rarely ellipsoid, without or with short collar, hyaline to pale brown, smooth-walled. Sporangiospores 3.5-6.5 × 2-4 µm (x - = 5 × 2.5 µm, n = 30) µm, mostly ellipsoidal, sometimes flattened on one side, oval or cylindrical, smooth-walled, hyaline with one or more granules. Chlamydospores abundant, intercalary, terminal, variable in shape and size. Rhizoids absent. Sexual morph not observed.

Culture characteristics.

Colonies on MEA reaching a diameter of 60 mm after 3 days of incubation at 25 °C. At temperatures 20 and 30 °C, the colony diameter is 46 mm and 45.5 mm respectively after 3 days. Colony growth is observed at 8 to 30 °C with optimal growth at 25 °C. Unbranched or sympodial branching of sporangiophore are prominent at 30 °C. Colonies on PDA, reaching 51 mm after 3 days of incubation at 25 °C. In both culture media, colony from above and reverse: white or very pale yellow, and cottony. No growth was observed at 37 °C. At 8 °C, the colony reached a diameter of 21 mm after 9 days but with no sporulation in MEA.

Distribution.

Thailand, United Kingdom.

Figures

Figure 1. Maximum likelihood phylogram inferred from 102 taxa and 2017 characters based on ITS, and LSU matrix using GTR + G + I model and partition analysis. Maximum likelihood bootstrap support (≥ 70 %) and Bayesian posterior probability (≥ 0.70) are indicated above the branches or near the nodes in this order. The tree is artificially rooted using Backusella dispersa (CBS 195.28), and B. grandis (CBS 186.87). The new species are in bold and the type species in the dataset are indicated using T. (-) represent bootstrap support lower than 70 % or posterior probability lower than 0.70.

Figure 4. Mucor nederlandicus (MFLU 21 - 007) a-c columella with highly reduced collar d sterile sporangium e columella with visible collar f-h chlamydospores i mature sporangia j sporangiospores k front and reverse of the colony in MEA. Scale bars: 20 µm (a-e, i); 10 µm (f-h).