Distoseptispora clematidis Phukhams., M.V. de Bult & K.D. Hyde, in Phukhamsakda et al., Fungal Diversity 102: 168 (2020)

Shen, Hong-Wei, Bao, Dan-Feng, Hyde, Kevin D., Su, Hong-Yan, Bhat, Darbhe J. & Luo, Zong-Long, 2021, Two novel species and two new records of Distoseptispora from freshwater habitats in China and Thailand, MycoKeys 84, pp. 79-101 : 79

Distoseptispora clematidis Phukhams., M.V. de Bult & K.D. Hyde, in Phukhamsakda et al., Fungal Diversity 102: 168 (2020)

Figure 4

Description.

Saprobic on submerged wood in freshwater River. Sexual morph: Undetermined. Asexual morph: Colonies on the substratum superficial, effuse, scattered, hairy, dark brown. Mycelium partly immersed in substrate, composed of branched, smooth, septate, brown to dark brown hyphae. Conidiophores 30-41 × 5-6 μm (x¯ = 36 × 6 μm, n = 15), macronematous, mononematous, single or in a small group, straight or slightly flexuous, unbranched, septate, erect, 2-4-septate, cylindrical, smooth, dark brown to brown. Conidiogenous cells 7-9 × 5-6 μm (x¯ = 8 × 5 μm, n = 15), monoblastic, integrated, determinate, terminal, cylindrical, pale brown to brown. Conidia 83-220 × 12-14 μm (x¯ = 151 × 13 μm, n = 20), acrogenous, solitary, obclavate, cylindrical, elongated, straight or curved, truncate at base, rounded at apex, 16-41-distoseptate, slightly constricted at some septa, smooth, brown to greenish-brown, thick-walled.

Culture characters.

Conidia cultivated on PDA within 12h and germ tubes produced at the ends. Colonies on PDA, attaining 4 cm after 1 month at room temperature (25 °C), gray at first, later becoming dark gray, loose, flocculent, smooth edge, dark brown on the reverse.

Material examined.

China, Yunnan Province, Kunming City, Yiliang County, Nanpanjiang River , 24°38'28"N; 103°09'38"E, on submerged decaying wood, 12 June 2018; Hongwei Shen and Xiu He, S-1797 (KUN-HKAS 112708), living culture (DLUCC 1797) .

Notes.

Our new isolate clustered with the ex-type strain of Distoseptispora clematidis (MFLU 17-1501) ( Phukhamsakda et al. 2020) with 97%ML/0.99PP bootstrap support (Figure 1 ). Distoseptispora clematidis (MFLU 17-1501) was collected on dead culms of Thysanolaena maxima (Roxb. ex Hornem.) Honda in Yunnan Province, China. Based on morphological analysis, the size and shape of the conidia and conidiophores of our new isolate are similar to D. clematidis . Therefore, we identified our new isolate as D. clematidis and it is a new record from freshwater habitats in China.

Figures

Figure 1. Maximum likelihood (ML) tree is based on combined of LSU, ITS, SSU, TEF 1 - α, and RPB 2 sequence data. Bootstrap support values with an ML greater than 70 % and Bayesian posterior probabilities (PP) greater than 0.97 given above the nodes, shown as " ML / PP ". The tree is rooted with Pseudostanjehughesia aquitropica (MFLUCC 16 - 0569) and P. lignicola (MFLUCC 15 - 0352). New species are indicated in red and type strains are in bold.

Figure 4. Distoseptispora clematidis (KUN-HKAS 112708) A colonies on the substratum B-C conidiophores with conidia D conidiogenous cells E-H conidia I germinating conidium J culture on PDA Scale bars: 30 μm (B, C, E-I); 20 μm (D).