Oxydothis fortunei H. M. Hu & Q. R. Li, 2022

Hu, Hong Min, Liu, Li Li, Zhang, Xu, Lin, Yan, Shen, Xiang Chun, Long, Si Han, Kang, Ji Chuan, Wijayawardene, Nalin N., Li, Qi Rui & Long, Qing De, 2022, New species and records of Neomassaria, Oxydothis and Roussoella (Pezizomycotina, Ascomycota) associated with palm and bamboo from China, MycoKeys 93, pp. 165-191 : 165

Oxydothis fortunei H. M. Hu & Q. R. Li sp. nov.

Fig. 7

Holotype.

GMB0315.

Etymology.

In reference to the host, Trachycarpus fortunei (Hook.) H. Wendl.

Description.

Saprobic on surface of culms of T. fortunei . Sexual morph: Ascomata 205-317 μm diam. (x̄ = 261 μm, n = 30), solitary or aggregated in groups, immersed, forming slightly raised as blistering areas on the host surface, long axis horizontal to that of the host, 18-41 μm high × 155-207 μm broad, in transverse section, ellipsoid, ostiolate, coriaceous, black, flat. Peridium 24-27 μm thick, composed of 2-3 several layers of flattened, light-brown cells. Asci 108-121 × 9-14 μm (x̄ = 114 × 12 μm, n = 20), 8-spored, unitunicate, cylindrical, mostly straight, pedicellate, with a J-, subapical apparatus, 4.2-4.9 μm high, 5.5-6.8 μm diam. Ascospores 56-72 μm × 3-4 μm (x̄ = 66 × 3.3 μm, n = 30), fusiform, hyaline, obliquely 1-2-seriate, tapering gradually from the center to the ends, with multi-guttules in each cell, pointed processes. Asexual morph: Undetermined.

Culture characteristics.

Ascospores germinated on PDA within 24 hours at 25 °C, colonies are reaching 4.5 cm diam. circular, transparent, thin, colony reverse is same. Not sporulating on OA nor on PDA.

Specimen examined.

China Guizhou Province, Long gong scenic spot (26°04'35.02"N, 105°52'15.04"E), on surface of culms of T. fortunei , 5 December 2021. Altitude: 1120m, Q.R. Li and X. Xu, 2021 LG9 (GMB0315, holotype; KUN-HKAS 123430, isotype; ex-type living culture GMBC0315).

Other examined material.

China, Guizhou Province, Long gong scenic spot (26°04'47.41"N, 105°31'10.34"E), on surface of culms of palm, 7 December 2021. Altitude: 1095m, Q.R. Li and X. Xu, 2021 LG15 (GMB0389; living culture GMBC0389) .

Notes.

Oxydothis fortunei is morphologically similar to O. nonamyloidea K.D. Hyde and O. rhapidicola S. Konta & K.D. Hyde in the shape of ascospores ( Hyde 1994; Hidayat et al. 2006; Konta et al. 2016). However, the ascospores of O. fortunei (56-72 × 3-4 μm) are shorter than those of O. nonamyloidea (94-115 × 3.5-4.5 μm) and O. rhapidicola (47-50 × 3-5 μm). Moreover, it is distinguished from O. rhapidicola since the latter has a blue slit-like ascus subapical apparatus in Melzer’s reagent ( Konta et al. 2016). Oxydothis fortunei showed the close kinship to O. inaequalis (100% ML, 1 BYPP; Fig. 3 ). However, O. fortunei differs from O. inaequalis by its shape of the ascospores, and the J- ascus subapical apparatus as well as the smaller ascospores (56-72 × 2.9-3.9 μm vs. 78-100 × 5-6 μm) ( Hidayat et al. 2006).

Figures

Figure 3. RAxML tree of Oxydothis and related genera based on a combined ITS, LSU and SSU sequences dataset. Bootstrap support values for ML equal to, or greater than, 60 % and BYPP equal to or greater than 0.95 are given above the nodes. The new collections are in red bold and ex-type strains are in bold.

Figure 7. Oxydothis fortunei (Holotype, GMB 0315) A stromata on host substrate B close-up of ascomata C cross-section of the ascomata D longitudinal section of an ascoma E peridium F-H asci I apical apparatus (stained in Melzer's Reagent) J, K ascospores. Scale bars: 0.5 mm (B-C); 10 μm (D-K).