Microhyla heymonsi Vogt, 1911

Mulcahy, Daniel G., Lee, Justin L., Miller, Aryeh H., Chand, Mia, Thura, Myint Kyaw & Zug, George R., 2018, Filling the BINs of life: Report of an amphibian and reptile survey of the Tanintharyi (Tenasserim) Region of Myanmar, with DNA barcode data, ZooKeys 757, pp. 85-152 : 85

Microhyla heymonsi Vogt, 1911

Description.

Adult female (n = 1) 25.1 mm, adult males (2) 20.1, 20.6 mm SVL.

Natural history notes.

From flooded fields.

General Distribution.

Northeast India through southern China to Taiwan, southward through Southeast Asia to Sumatra.

Molecular Data.

We sequenced four other individuals, two from Bago (USNM 587130, MBM- USNMFS 35509) and two from Mandalay (USNM 587138, USNM 587140). These were each placed in their own COIBIN, as were our specimens from the Tanintharyi. These were similar to our Tanintharyi specimens based on 16S data and were all placed in a 16S clade with specimen in GenBank identified as M. heymonsi . There were two distinct clades within the GenBank M. heymonsi material; ours were placed in one with other specimens from Myanmar (e.g. KC179993), and Singapore (e.g. HM359093). Sheridan et al. (2010) identified three clades within M. heymonsi . These data indicate this represents a species complex in need of further revision.

Specimens examined.

USNM 586955-957, USNM 587130, USNM 587138, USNM 587140.

Red List status.

LC.

Additional Microhyla .

We sequenced nine additional specimens of Microhyla from northern Myanmar. Four (USNM523975, USNM523976, USNM523979, USNM 537450) from Sagaing did not match any species description, were placed in their own COIBIN, were placed in their own clade in the 16S tree, and likely represent a new species ( M. sp. A.). One identified as M. rubra was placed in a COIBIN with another individual identified as M. rubra from Myanmar and the 16S sequence is identical to a specimen in GenBank (KM509166) from Magway, Myanmar. Other specimens identified as M. rubra in GenBank were placed elsewhere in the tree. However, six of these (KU214856-61) represent a recently described species ( M. mihintalei , Wijayathilaka et al. 2016), while the other two (AB201192 and KU214855) represent M. rubra from Sri Lanka and India (Karnataka). The type locality for M. rubra is "in the Carnatic near rivers, in sandy banks … also Ceylon" India and Sri Lanka (fide Frost 2017). Therefore, our specimen, the KM509166, and the other specimen in the BOLDBIN (BOLD:ACW0810) likely represent a new species. We refer to our specimen as M. sp. B. Two others identified as M. berdmorei from Yangon (MBM-JBS19917MBM-JBS 19929), and two M. berdmorei from Bago (MBM- USNM-FS 35556, USNM 587407) were placed in the same COIBIN, and were placed in a 16S clade identical to one from Sagaing, Myanmar (KC179981; de Sá et al. 2012) and similar to three others identified as "M. sp. B MS-2009" from Bangladesh ( Hasan et al. 2012a). This clade was placed sister to two other clades identified as M. berdmorei (and one M. fowleri ), which suggests a species complex in need of revision. We sequenced a paratype (USNM 523965) of Kalophrynus anya ( Zug 2015) and one Glyphoglossus molossus (USNM 523961), both from Sagaing, Myanmar.