Tremella latispora F. Wu, L.F. Fan & Y. C. Dai, 2021

Fan, Long-Fei, Alvarenga, Renato Lucio Mendes, Gibertoni, Tatiana Baptista, Wu, Fang & Dai, Yu-Cheng, 2021, Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota), MycoKeys 82, pp. 33-56 : 33

Tremella latispora F. Wu, L.F. Fan & Y. C. Dai sp. nov.

Figs 3E , 7

Holotype.

China. Yunnan, Xinping, Shimenxia Park, on stump of Lithocarpus , 16 June 2017, Y.C. Dai 17574 (BJFC025106).

Etymology.

Refers to the species having wide basidiospores.

Basidioma.

Sessile, when fresh soft gelatinous, creamy-white to lvory, translucent, pustulate to irregularly cerebriform, with thick and undulate lobes, up to 4.0 cm long, 2.0 cm broad and 1.0 cm high from base, distinctly shrinking into a film and becoming whitish to pale yellow when dry, broadly attached to substratum.

Internal features.

Hyphae hyaline, smooth, thin- to thick-walled, 1.5-6.0 µm in diameter, branched, interwoven, with abundant clamp connections, clamp complexes and anastomoses, thick-walled hyphae usually present near to base of basidioma and sometimes swollen up to 7.5 μm; hyphidia hyaline, smooth, thin-walled, usually derived from the same hyphae with basidia; swollen cells, vesicles and haustoria absent; mature basidia thin-walled, globose to subglobose, with a basal clamp connection, 17.2-24.0(-27.0) × 17.0-23.0(-24.3) μm, L = 19.5 µm, W = 20.8 µm, Q = 0.94 (n = 30/1), commonly their width greater than length, usually longitudinally septate, occasionally obliquely septate, 2-4-celled, with obvious oil drops; sterigmata up to 60 μm long, 1.5-2.0 in diameter, slightly protuberant at apex; probasidia thin-walled, ellipsoid to subglobose, proliferating from terminal hyphae; basidiospores hyaline, thin-walled, globose to subglobose, apiculate, with oil drops, (9.0-)10.1-11.8(-12.0) × (9.6-)9.9-11.4(-11.7) μm, L = 11.0 µm, W = 10.7 µm, Q = 1.03 (n = 30/1), germination by germ tubes or secondary spores; conidia massively present, originating from umbelliform conidiophores, hyaline, thin-walled, ovoid to oblong or globose to subglobose, 2.8-3.6 × 1.8-3.0 μm.

Additional specimen examined.

(paratype) China Yunnan, Xinping, Shimenxia Park, on stump of Lithocarpus , 16 June 2017, Y.C. Dai 17568 (BJFC025100).

Notes.

Phylogenetically, Tremella latispora formed a distinct lineage closely related to T. cheejenii (Figs 1 , 2 ). Morphologically, the species has significantly larger basidia and basidiospores than T. cheejenii or other similar species (Table 3 ), and it has globose to subglobose basidiospores rather than more or less ellipsoid basidiospores in other species. And T. latispora are different from T. cheejenii and T. fibulifera s.s. by 4.63% and 5.09% sequence differences in the ITS sequences and 3.39% and 2.95% in the partial nLSU sequences respectively.

Figures

Figure 1. The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s. s. based on the ITS + partial nLSU dataset. Bootstrap support values for MP and ML greater than 50 % and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

Figure 2. The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s. s. based on the combined ITS + partial nLSU + mtSSU + TEF 1 + RPB 1 + RPB 2 dataset. Bootstrap support values for MP and ML greater than 50 % and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

Figure 3. Basidioma A Tremella fibulifera (Alvarenga 471) B T. australe (Wu 154) C T. guangxiensis (Wu 3) D T. latispora (Dai 17568) E T. " Tremella neofibulifera " (Wu 244) F T. subfibulifera (Alvarenga 334). Scale bars: 1 cm (A-F).

Figure 7. Microscopic structures of Tremella latispora (Dai 17568) A basidiospores B germination tubes of basidiospores and secondary spores C conidia and conidiophores D, E basidia F, G probasidia H, I hyphae with clamp connections and clamp complexes. Scale bars: 10 μm (A-I).