Neopestalotiopsis saprophytica (Maharachch. & K.D. Hyde) Maharachch., K.D. Hyde & Crous, 2014

Yang, Qi, Zeng, Xiang-Yu, Yuan, Jun, Zhang, Qian, He, Yu-Ke & Wang, Yong, 2021, Two new species of Neopestalotiopsis from southern China, Biodiversity Data Journal 9, pp. 70446-70446 : 70446

Neopestalotiopsis saprophytica (Maharachch. & K.D. Hyde) Maharachch., K.D. Hyde & Crous, 2014

Materials

Type status: Other material. Occurrence: recordedBy: Qi Yang; occurrenceID: GUCC 21506; Taxon : scientificName: Neopestalotiopsis saprophytica; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Guangxi; locality: Nanning City ， Guangxi Medicinal Botanical Garden ; verbatimCoordinates: 108°19' E, 22°51' N; Identification : identifiedBy: Qi Yang ; dateIdentified: 2021; Record Level : collectionID: HGUP 423 Type status: Other material. Occurrence: recordedBy: Qi Yang; occurrenceID: GUCC 21507; Taxon : scientificName: Neopestalotiopsis saprophytica; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Guangxi; locality: Nanning City , Guangxi Medicinal Botanical Garden ; verbatimCoordinates: 108°19' E, 22°51' N; Identification: identifiedBy: Qi Yang; dateIdentified: 2021; Record Level: collectionID: HGUP 133

Description

Disease symptom: Pathogenic causing spots on leaves of Erythropalum scandens . Leaf spots shape irregular, brown to reddish-brown, slightly sunken spots appear on surface leaves of E. scandens , which scattered on the leaves tip. Small brown spots appeared initially and then gradually enlarged, changing to reddish-brown spots with a yellow border.

Colonies on PDA reaching 7.5-8 cm in diam. after 7 d at room temperature (28℃), under light 12 hr/dark. Hyphae change from light pink to off-white. Colonies filamentous to circular, slightly undulate at the edge, with black fruiting bodies clustered, filiform margin, light pink from above and light yellow from the reverse. Sexual morph: undetermined. Asexual morph (Fig. 3 ): Conidiomata up to 280 μm in diam., pycnidial, globose, solitary, black, semi-immersed on PDA, exuding brown to dark brown mass of conidia. Conidiophores branched or unbranched, hyaline, thin-walled. Conidiogenous cells discrete, ampulliform to lageniform, hyaline, thin-walled, smooth. Conidia (21.5-)26.5 × 4.5(-6.5) µm (x̄ = 23.2 × 5.2 µm, n = 30), fusiform to clavate, straight to slightly curved, 4-septate; basal cell obconic, hyaline or sometimes pale brown, thin-walled, smooth, 3-5 µm (x̄ = 4 µm, n = 30); the three median cells 13-17 µm (x̄ = 14.9 µm, n = 30), pale brown to brown, dark brown with septa darker than the rest of the cells, the second cell from base 4-6.5 µm (x̄ = 4.9 µm, n = 30); the third cell 3-5 µm (x̄ = 4.1 µm, n = 30); the fourth cell 3.5-6 µm (x̄ = 4.8 µm, n = 30); apical cell 3-5 µm (x̄ = 3.9 µm, n = 30), cylindrical, hyaline; 1-4 (mostly 3) tubular apical appendages, arising from the apex of the apical cell each at different point, flexuous, 18-28.5 µm (x̄ = 22.4 µm, n = 30); basal appendage present most of the time, single, tubular, unbranched, 3.3-7 µm (x̄ = 4.3 µm, n = 30).

Notes

GUCC 21506 and GUCC 21507 with the same nucleotides sequences were related to N. dendrobii (MFLUCC 14-0106) and N. saprophytica (CBS 115452). There were ten character differences with N. dendrobii and 11 character differences with N. saprophytica , but the most differences (nine character differences) between our strains and N. saprophytica were only in the tef1 region. Alternatively, collection differed to N. dendrobii in having more apical appendages ( N. dendrobii : 2-3) and much longer apical appendages ( N. dendrobii : 6 ± 0.9 µm) ( Ma et al. 2019). Morphological characters of our collections and N. saprophytica overlapped ( Maharachchikumbura et al. 2014). Thus, GUCC 21506 and GUCC 21507 are considered as N. saprophytica .

Figures

Figure 3. Neopestalotiopsis saprophytica (GUCC 21506). a, b, c. Leaf spots of Neopestalotiopsis saprophytica; d, e. Culture on PDA (d - above, e - reverse); f. Colony sporulating on PDA; g-h. Conidia and conidiophores; i-m. Conidia. Scale bars: f = 1000 µm, g-m = 20 µm.