Nemania changningensis Y.H. Pi & Q.R. Li, 2021

Pi, Yin Hui, Long, Si Han, Wu, You Peng, Liu, Li Li, Lin, Yan, Long, Qing De, Kang, Ji Chuan, Kang, Ying Qian, Chang, Chu Rui, Shen, Xiang Chun, Wijayawardene, Nalin N., Zhang, Xu & Li, Qi Rui, 2021, A taxonomic study of Nemania from China, with six new species, MycoKeys 83, pp. 39-67 : 39

Nemania changningensis Y.H. Pi & Q.R. Li sp. nov.

Fig. 4

Etymology.

Refers to the collection location, Changning County.

Material examined.

China, Yunnan Province, Changning County, Lancang River Nature Reserve (25°01'35.02"N, 99°33'15.42"E, altitude: 2670 m), on dead wood, 3 October 2019, Y.H. Pi, 2019LC203 (GMB0056, holotype; GMBC0056, ex-type living culture; KUN-HKAS 112668, isotype) .

Description.

Saprobic on the surface of decaying wood. Sexual morph: Stromata effused-pulvinate, confluent into irregularly elongated compound stromata, up to 18-35 mm long × 2-4 mm wide × 0.3-0.5 mm high, irregularly lobed, plane or with inconspicuous perithecial mounds and sloping margins; surface covered with white tissue, persistent layer, with blackish-grey carbonaceous sub-surface showing through in places; the tissue beneath the perithecial layer inconspicuous, greyish-white in places, the underlying wood blackened; mature stromata lacking KOH extractable pigments. Perithecia 0.45-0.6 mm diam. × 0.4-0.55 mm high, subglobose to depressed-spherical. Ostioles slightly higher than stromatal surface and with openings papillate, often surrounded by white tissue, inconspicuous, black, without encircling disc. Asci 100-140 × 7-10 μm (av. = 111 × 8.5 μm, n = 30), 8-spored, unitunicate, cylindrical, short-stipitate, the spore-bearing parts 70-90 µm long, the apical apparatus of immature asci blue in Melzer’s Reagent, but not blue in mature asci. Ascospores 10-13 × 4-6.5 μm (av. = 11.5 × 5.5 μm, n = 30), uniseriate unicellular, smooth, light brown, slightly inequilateral, with broadly rounded ends, inconspicuous or lack a germ slit; perispore indehiscent in 10% KOH. Asexual morph: Undetermined.

Culture characteristics.

The colony grows slowly on the PDA with a diameter of 4.5 cm after 2 weeks at 25 °C. The colony on the surface is white, thick and flat in the middle, edges are shallow, irregular bands and rosettes. Colony reverse is orange and intermediate colour darker. Not sporulating on OA nor on PDA.

Other examined material.

China, Yunnan Province, Changning County, Lancang River Nature Reserve (25°01'30.36"N, 99°35'30.53"E, altitude: 2586 m), on dead wood, 4 October 2019, Y.H. Pi, 2019LC342 (GMB0057), living culture, GMBC0057 .

Notes.

In the phylogenetic analyses, N. changningensis is on a separate branch and grouped with N. caries with high support values (100% ML, 1 BYPP, Fig. 1 ). In term of ascospores dimension, N. changningensis resembles N. caries , but differs in the perithecia of N. caries (obovoid, 0.3-0.6 mm diam. × 0.5-0.7 mm high), in the surface not covered with white tissue and in its apical apparatus of mature asci bluing in Melzer’s Reagent ( Miller 1961; Ju and Rogers 2002).

Figures

Figure 1. RAxML tree based on analysis of a combined dataset of ITS, α-actin, rpb 2 and β-tubulin sequences from taxa of Nemania and related genera. Bayesian posterior probability (PP) ≥ 0.90 is marked at the node and the maximum likelihood bootstrap support (BS) values greater than ≥ 75 %; a dash (" - ") indicates a value <0.90 (PP) or <75 % (BS). The strain number is indicated after the species name. The here-studied strains are in bold and new species are indicated in red.

Figure 4. Nemania changningensis (GMB 0056, holotype) A type material B, C stromata on the surface of host D transverse sections of stromata E longitudinal section of stroma F-H asci with ascospores I pigments in 10 % KOH J, K asci apical apparatus (stained in Melzer's Reagent) L, M ascospores N, O colonies on PDA (N - upper, O - lower). Scale bars: 0.5 mm (C-E); 10 μm (F-H, J-M).