Toxoplasma gondii

Yue, Chanjuan, Yang, Wanjing, Li, Yunli, Zhang, Dongsheng, Lan, Jingchao, Su, Xiaoyan, Li, Lin, Liu, Yiyan, Zheng, Weichao, Wu, Kongju, Fan, Xueyang, Yan, Xia, Hou, Rong & Liu, Songrui, 2022, Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca), International Journal for Parasitology: Parasites and Wildlife 18, pp. 287-291 : 288

2.2. Detection of antibodies to T. gondii

2.2.1. Modified agglutination test (MAT)

All the 100 sera samples were tested for T. gondii antibodies using the commercial T. gondii MAT kit (University of Tennessee Research Foundation, Technology Transfer & Licensing, Memphis, USA) with a cut-off titer of 25, the experimental procedure was performed according to the manufacturer’ s instructions. Briefly, the experiment was carried out on 96-well U-bottom microtiter plate, the samples were diluted in twofold serial dilutions from 1: 25 to 1: 200, then the premixed reaction reagent was added to each well, the plate was then placed in a humid incubator at 37 ◦ C for 24 h. A positive result was noted when the reaction showed a layer of agglutinated tachyzoites covering more than half of the reaction well’ s bottom. A negative result was noted when the tachyzoites sedimentary formed a small button at the reaction well’ s bottom.