Cladosporium endophyticum Tibpromma & K.D. Hyde

Tibpromma, Saowaluck, Hyde, Kevin D., Bhat, Jayarama D., Mortimer, Peter E., Xu, Jianchu, Promputtha, Itthayakorn, Doilom, Mingkwan, Yang, Jun-Bo, Tang, Alvin M. C. & Karunarathna, Samantha C., 2018, Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand, MycoKeys 33, pp. 25-67 : 36

Cladosporium endophyticum Tibpromma & K.D. Hyde sp. nov. Figure 8

Etymology.

named after its status as an endophytic fungus.

Holotype.

MFLU 18-0005

Description.

Colonies on PDA attaining 9 cm diam. in six weeks at room temperature, slow growing, dark olivaceous. Mycelium superficial and immersed composed of septate, branched, 2.3-4.5 µm wide, sub-hyaline, with smooth and thick-walled hyphae. Sexual morph Undetermined. Asexual morph Conidiophores 6-10 µm high, 3-4 µm diam. (x̄ = 8.24 × 3.52 µm, n = 10), terminal and intercalary, cylindrical or sub-cylindrical, darkened conidiogenous loci. Conidia 3-6 × 2-4 µm (x̄ = 3.64 × 2.75 µm, n = 30), forming long branched chains, hyaline to pale-olivaceous, smooth and thin-walled, aseptate, globose to ovoid with rounded ends.

Culture characteristics.

Colonies on PDA (Figure 2, PE58), superficial, dark olivaceous with dark-grey centre, irregular, undulate with wrinkled and raised on surface media; reverse dark olivaceous. Generative hyphae simple-septate, branched, sub-hyaline, guttules, thick-walled (Figure 8).

Material examined.

THAILAND, Chumphon, Pathio District, on healthy leaves of Pandanus sp. ( Pandanaceae ), 1 December 2016, S. Tibpromma PE58 (MFLU 18-0005, holotype); HKAS100855, paratype, ex-type living cultures, MFLUCC 17-0599 = KUMCC 17-0294.

GenBank numbers.

ITS=MG646956, LSU=MG646949, SSU=MG646981, TEF1=MG646988.

Notes.

Cladosporium endophyticum was isolated as an endophyte from Pandanus sp. in Thailand. In the phylogenetic analysis of combined gene sequence data of ITS, LSU, SSU and TEF1, the new taxon Cladosporium endophyticum is sister to C. halotolerans (Figure 7), but well-separated with high bootstrap support (90% in ML). Moreover, the morphology of this new taxon was compared with Cladosporium halotolerans which has brown to dark brown, subglobose to globose with verrucose, less often short-ovoid conidia, narrower at both ends ( Zalar et al. 2007), while C. endophyticum has globose to ovoid, hyaline to pale-olivaceous conidia with rounded ends. Here, the authors introduce the new species C. endophyticum and provide an updated phylogenetic tree for the genus Cladosporium .