Legeriomyces ramosus Pouzar, 1972.

Valle, Laia Guardia & Stoianova, Desislava, 2020, First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia, MycoKeys 67, pp. 55-80 : 55

Legeriomyces ramosus Pouzar, 1972. Fig. 16

Specimens examined.

Site 2: slide BUL-2-9; Site 11: slides BUL-11-1 (zygo.), BUL-11-2, BUL-11-3.

Notes.

This species was found on the hindgut lining of Baetidae hosts ( Baetis rhodani Pictet, Baetis melanonyx (Pictet) and B. alpinus (Pictet). The species seems to have a cosmopolitan distribution, at least in the Northern Hemisphere ( Lichtwardt et al. 2001). In Europe, the species is common, with records from France, where it was originally described under the name of Genistella ramosa ( Léger and Gauthier 1932) ( Pouzar 1972), from United Kingdom ( Moss 1979), Switzerland ( Lichtwardt 1986), Spain ( Valle and Santamaria 2002), Norway ( White and Lichtwardt 2004), Sweden ( Lichtwardt 1984), Portugal ( Valle 2013a) and Italy ( Valle et al. 2013). Legeriomyces ramosus has been reported also from India ( Misra and Tiwari 2008), China ( Strongman et al. 2010), from several localities in USA ( Lichtwardt 1986) and Canada ( Strongman 2010). Bulgarian specimens have, as is usual in this species, a broad range of trichospore variability, measuring 30-40 × 7-8.5 µm, with two appendages differing in length. Zygospores measure 50-61 × 9-12 µm in our collections (Fig. 16 ).

Figures

Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures.