Longior longior, Morffe, Jans & Garcia, Nayla, 2011

Morffe, Jans & Garcia, Nayla, 2011, On some Cuban species of the genus Longior Travassos & Kloss, 1958 (Oxyurida, Hystrignathidae), with description of a new species, ZooKeys 78, pp. 1-14 : 3-9

Longior longior sp. n. Figure 2 A–GFigure 3 A–DFigure 5 A–F

Type material.

♀ holotype; Cuba, La Habana Province, San José de Las Lajas, La Jaula; in Passalus pertyi ; 15.III.2008; E. Fonseca, J. Morffe, F. Alvarez coll.; CZACC 11.4487. ♂ allotype, same data as holotype, CZACC 11.4620. 11 adult ♀♀ paratypes, same data as holotype, CZACC 11.4488-11.4498. 5 immature ♀♀ paratypes, CZACC 11.4499-11.4503, same data as holotype. 5 ♂♂ paratypes, same data as holotype, CZACC 11.4621-11.4625.

Other materials examined.

4 ♂♂ vouchers; Cuba, Holguín Province, Nipe-Sagua-Baracoa, La Melba; in Passalus pertyi ; V.2007; R. Barba, D. Ortiz coll.; CZACC 11.4626-11.4629.

3 ♀♀ paratypes of Longior zayasi ; Cuba, Pinar del Río Province, Sierra del Rosario, El Salón; in Passalus interstitialis; 1989; A. Coy, M. Alvarez coll.; CZACC 11.4178-11.4180.

Measurements.

Holotype (female) a = 23.78, b = 4.63, c = 7.04, V% = 51.70, total length = 3.520, maximum body width = 0.148, first cephalic annule ( length×width) = 0.028 × 0.035, stoma length = 0.078, procorpus length = 0.640, isthmus length = 0.045, diameter of basal bulb = 0.070, total length of oesophagus = 0.760, nerve ring to anterior end = 0.233, excretory pore to anterior end = 0.980, vulva to posterior end = 1.700, anus to posterior end = 0.500, eggs = 0.123 –0.128×0.045– 0.053 (0.125 ± 0.003 × 0.049 ± 0.004 n = 3).

Female adult paratypes (n = 11) a = 23.61-28.28 (25.00 ± 1.57 n = 11), b = 4.49-5.45 (4.81 ± 0.29 n = 10), c = 6.24-8.54 (7.37 ± 0.66 n = 11), V% = 49.73-58.01 (52.98 ± 2.34 n = 11), total length = 3.500-4.525 (3.863 ± 0.277 n = 11), maximum body width = 0.138-0.173 (0.155 ± 0.010 n = 11), first cephalic annule ( length×width) = 0.023 –0.028×0.035– 0.038 (0.024 ± 0.002 × 0.036 ± 0.001 n = 9), stoma length = 0.073-0.085 (0.079 ± 0.005 n = 11), procorpus length = 0.630-0.720 (0.678 ± 0.027 n = 10), isthmus length = 0.040-0.058 (0.053 ± 0.005 n = 10), diameter of basal bulb = 0.065-0.078 (0.071 ± 0.004 n = 10), total length of oesophagus = 0.760-0.860 (0.806 ± 0.030 n = 10), nerve ring to anterior end = 0.230-0.250 (0.242 ± 0.007 n = 9), excretory pore to anterior end = 0.970-1.125 (1.050 ± 0.047 n = 10), vulva to posterior end = 1.600-1.925 (1.813 ± 0.097 n = 11), anus to posterior end = 0.470-0.590 (0.526 ± 0.038 n = 11), eggs = 0.110 –0.143×0.050– 0.060 (0.133 ± 0.006 × 0.053 ± 0.003 n = 28).

Female immature paratypes (n = 5) a = 24.31-27.02 (24.96 ± 1.16 n = 5), b = 4.13-4.42 (4.28 ± 0.12 n = 5), c = 7.07-8.86 (7.74 ± 0.68 n = 5), V% = 53.54-56.56 (54.99 ± 1.07 n = 5), total length = 3.050-3.325 (3.165 ± 0.104 n = 5), maximum body width = 0.118-0.135 (0.127 ± 0.006 n = 5), first cephalic annule ( length×width) = 0.023 –0.025× 0.035 (0.024 ± 0.001 × 0.035 ± 0.000 n = 5), stoma length = 0.070-0.073 (0.072 ± 0.001 n = 5), procorpus length = 0.610-0.680 (0.638 ± 0.028 n = 5), isthmus length = 0.055-0.058 (0.056 ± 0.001 n = 5), diameter of basal bulb = 0.063-0.068 (0.065 ± 0.002 n = 5), total length of oesophagus = 0.690-0.760 (0.740 ± 0.029 n = 5), nerve ring to anterior end = 0.223-0.233 (0.227 ± 0.004 n = 5), excretory pore to anterior end = 0.910-0.980 (0.942 ± 0.031 n = 5), vulva to posterior end = 1.325-1.500 (1.425 ± 0.068 n = 5), anus to posterior end = 0.350-0.470 (0.412 ± 0.045 n = 5).

Allotype (male) a = 15.68, b = 3.63, c = 49.67, total length = 1.490, maximum body width = 0.095, stoma length = 0.038, procorpus length = 0.330, isthmus length = 0.033, diameter of basal bulb = 0.045, total length of oesophagus = 0.410, nerve ring to anterior end = 0.160, excretory pore to anterior end = 0.560, cloaca to posterior end = 0.030, distance from the mammiform papillae to the posterior end = 0.130, distance from the dorso-lateral papillae to the posterior end = 0.065.

Male paratypes (n = 5) a = 11.56-14.10 (12.40 ± 1.04 n = 5), b = 3.13-3.60 (3.38 ± 0.17 n = 5), c = 43.33-51.27 (47.13 ± 3.54 n = 5), total length = 1.220-1.510 (1.366 ± 0.111 n = 5), maximum body width = 0.100-0.120 (0.111 ± 0.010 n = 5), stoma length = 0.040-0.048 (0.043 ± 0.003 n = 5), procorpus length = 0.300-0.340 (0.322 ± 0.018 n = 5), isthmus length = 0.030-0.033 (0.031 ± 0.001 n = 5), diameter of basal bulb = 0.045-0.048 (0.047 ± 0.001 n = 5), total length of oesophagus = 0.390-0.420 (0.404 ± 0.015 n = 5), nerve ring to anterior end = 0.150-0.168 (0.159 ± 0.007 n = 5), excretory pore to anterior end = 0.480-0.550 (0.512 ± 0.028 n = 5), cloaca to posterior end = 0.028-0.030 (0.029 ± 0.001 n = 5), distance from the mammiform papillae to the posterior end = 0.128-0.148 (0.137 ± 0.010 n = 3), distance from the dorso-lateral papillae to the posterior end = 0.058-0.065 (0.062 ± 0.003 n = 5).

Population from La Melba, Nipe-Sagua_Baracoa, Holguín Province.

Males (n = 4) a = 11.55-14.00 (13.00 ± 1.12 n = 4), b = 3.10-4.20 (3.50 ± 0.48 n = 4), c = 33.87-61.09 (44.96 ± 11.49 n = 4), total length = 1.170-1.680 (1.348 ± 0.227 n = 4), maximum body width = 0.085-0.120 (0.104 ± 0.015 n = 4), stoma length = 0.038-0.040 (0.039 ± 0.001 n = 4), procorpus length = 0.283-0.320 (0.303 ± 0.016 n = 4), isthmus length = 0.028-0.038 (0.032 ± 0.004 n = 4), diameter of basal bulb = 0.050-0.063 (0.054 ± 0.006 n = 4), total length of oesophagus = 0.378-0.400 (0.384 ± 0.010 n = 4), nerve ring to anterior end = 0.133-0.158 (0.145 ± 0.013 n = 3), excretory pore to anterior end = 0.430-0.490 (0.463 ± 0.025 n = 4), cloaca to posterior end = 0.028-0.038 (0.031 ± 0.005 n = 4), distance from the mammiform papillae to the posterior end = 0.115-0.133 (0.125 ± 0.009 n = 3), distance from the dorso-lateral papillae to the posterior end = 0.055-0.070 (0.063 ± 0.006 n = 4).

Population from El Salón, Sierra del Rosario, Pinar del Río Province.

Females (n = 3) a = 17.20-23.94 (21.67 ± 3.87), b = 4.79-4.86 (4.83 ± 0.05), c = 6.68-7.63 (7.25 ± 0.51), V% = 45.99-52.19 (50.04 ± 3.51), total length = 3.580-3.890 (3.737 ± 0.155), maximum body width = 0.150-0.218 (0.177 ± 0.036), first cephalic annule = 0.018 –0.025×0.035– 0.038 (0.021 ± 0.005 × 0.036 ± 0.002), stoma length = 0.060-0.075 (0.066 ± 0.008), procorpus length = 0.650-0.660 (0.655 ± 0.007), isthmus length = 0.050-0.058 (0.053 ± 0.004), diameter of basal bulb = 0.070-0.073 (0.071 ± 0.002), total length of oesophagus = 0.780-0.800 (0.790 ± 0.014), nerve ring to anterior end = 0.225-0.235 (0.230 ± 0.005), excretory pore to anterior end = 0.990-1.05 (1.020 ± 0.042), vulva to posterior end = 1.720-2.020 (1.867 ± 0.150), anus to posterior end = 0.480-0.560 (0.517 ± 0.040), eggs = 0.128 –0.140×0.045– 0.060 (0.133 ± 0.005 × 0.053 ± 0.006 n = 5).

Description.

Female. Body long and relatively slender. Cervical cuticle without spines. Subcuticular longitudinal striae present. Lateral alae well developed, arising from about two body widths posterior to the basal bulb up to the level of the anus. Head rounded, set-off from body by a single, deep groove, bearing eight prominent, paired papillae. First cephalic annule slightly inflated, about 2 to 2.5 head-lengths long. Stoma long, slender, about 3.5 first cephalic annule lengths long, surrounded by an esophageal collar. Oesophagus consisting of a very elongated, subcylindrical, muscular procorpus less set-off from the isthmus. Basal bulb sub-spherical, valve plate well developed. Intestine simple, sub-rectilinear, its fore region slightly inflated. Rec tum short, anus not prominent. Nerve ring encircling procorpus at about 20% of its length. Excretory pore located at about a body width posterior to basal bulb. Vulva a median transverse slit, near midbody, lips not prominent. Vagina muscular, strongly developed, forwardly directed. Genital tract monodelphic-prodelphic. Ovary reflected at about two body widths posterior to bulb, distal flexure about two body widths long. Eggs ovoid, with eight, rough, very prominent, longitudinal ridges not reaching the poles. Oocytes in a single row. Tail conical, subulate, ending in a fine tip.

Male. Body comparatively stout, shorter than female. Posterior region of body ventrally curved. Cervical cuticle unarmed, visibly annulated up to a little distance before the nerve ring. Sub-cuticular longitudinal striae present. Head set off from body by a single groove and bearing eight small, paired papillae. First cephalic annule not conspicuous. Stoma long, surrounded by an esophageal collar. Oesophagus slightly sub-fusiform, diminishing its diameter toward its second half until equaling the diameter of the isthmus. Basal bulb rounded, valve plate developed. Nerve ring encircling procorpus at about 35% of its length. Excretory pore situated at little more than a body-width posterior to basal bulb. Testis single, full of spermatids, commencing at about 1.5 body-widths posterior to bulb. Spicule absent. A large, median, mammiform precloacal papillae situated at a little less than a body width before the posterior end. A pair of dorso-lateral precloacal papillae located at about 0.5 body-widths before the tail tip. Dorsal cuticle of posterior end thickened from level of the latter pair of papillae to the tail tip. There is a cuticular bursa-like structure in the tail end, almost reaching the level of the tail tip.

Differential diagnosis.

Longior longior sp. n. differs from Longior longicollis , from Brazil,by having females with the body larger (3.500-4.525 vs. 2.98-3.64) and the tail comparatively longer (c = 6.72-8.54 vs. 9.03-9.33). The vulva is slightly displaced forward in the present species (V% = 50.00-58.01 vs. 57.72-61.81). The tail of the male of Longior longior sp. n. is comparatively shorter (c = 43.33-51.27: 30.66-32.83), and has the tip unreflexed. In Longior longior the nerve ring is displaced to the first half of the procorpus in both sexes, instead of Longior longicollis sp. n. where this structure is situated in the midpoint of the procorpus.

Longior longior sp. n. differs from Longior similis by lacking wide and marked annule in the cervical cuticle of females. Its body is longer (total length = 3.500-4.525 vs. 2.675-3.075) and the first cephalic annule is more inflated. The ovary is reflexed at about two body widths beyond the excretory pore, more posterior than Longior similis where it is reflexed at little less than a body width beyond the excretory pore. Males of the new species differ from Longior similis by having the cervical cuticle markedly annulated instead of Longior similis that presents the cervical cuticle smooth.

Longior longior sp. n. is distinguished from Longior alius García & Coy, 1994 (from Cuba) by having the isthmus shorter and not bent, instead of the isthmus very long and bent of the latter species. From Longior semialata Hunt, 1981, from Saint Lucia, West Indies, differs by the lateral alae that commence before the level of the vulva, the body longer (total length = 3.500-4.525 vs. 2.26-2.63) and the oesophagus comparatively shorter (b = 4.49-5.45 vs. 3.30-3.70). The lateral alae of Longior semialata start at about the middle of the distance between the vulva and the anus.

Longior longior sp. n. can be differentiated from Longior elieri García, Ventosa & Morffe, 2009 (from Cuba) by having an inflated first cephalic annule and by lacking of wide annule in the cervical region. Longior elieri has a not inflated first cephalic annule and the first third of the cervical cuticle widely annulated.

Etymology.

Named after the Latin longior: the largest, being this species the largest in the genus.

Type host.

Passalus pertyi ( Coleoptera , Passalidae )

Other host.

Passalus interstitialis Escholtz, 1829 ( Coleoptera , Passalidae )

Site.

Gut caeca

Type locality.

La Jaula, San José de Las Lajas, La Habana Province, Cuba.

Other records

(formerly referred as L. zayasi). El Salón, Sierra del Rosario, Pinar del Río Province, Cuba ( Coy et al. 1993); El Mulo, Sierra del Rosario, Pinar del Río Province, Cuba ( García et al. 2009a); La Melba, Nipe-Sagua-Baracoa, Holguín Province, Cuba ( García et al. 2009b); La Platica, Sierra Maestra, Granma Province, Cuba ( Morffe et al. 2009).

Remarks.

All the records of females of Longior longior sp. n. were formerly referred as Longior zayasi and measures of most of them have been published. In order to compare the meristic features between localities a chart with such variables is offered (Table 1).