Sporothrix tumida R. Jankowiak & P. Bilanski, 2023

Bilanski, Piotr, Jankowiak, Robert, Solheim, Halvor, Fortuna, Pawel, Chyrzynski, Lukasz, Warzecha, Paulina & Taerum, Stephen Joshua, 2023, Soil-borne Ophiostomatales species (Sordariomycetes, Ascomycota) in beech, oak, pine, and spruce stands in Poland with descriptions of Sporothrix roztoczensis sp. nov., S. silvicola sp. nov., and S. tumida sp. nov., MycoKeys 97, pp. 41-69 : 41

Sporothrix tumida R. Jankowiak & P. Bilanski sp. nov.

Fig. 8

Etymology.

Referring to the Latin tumeo (swollen) to reflect the characteristically inflated hyphae and conidiogenous cells.

Diagnosis.

Sporothrix tumida differs from the phylogenetically closely related S. macroconidia in respect of dimensions of its conidia.

Type.

Poland, Podkarpackie Province, Mielec , from fallen shoots of Pinus sylvestris pruned by Tomicus sp., October 2007, P. Bilański, (O-F- 259433 holotype, culture ex-type CBS 147970) .

Description.

Sexual morph not observed. Asexual structures produced on sterilized Scots pine twigs placed on the surface of malt agar in Petri dishes. Conidiophores hyaline, one- or two-celled, micronematous, simple or slightly branched, either borne on vegetative hyphae or on upright hyphae, often inflated. Conidiogenous cells blastic, cylindrical, terminal, straight, constricted at the base and strong tapering towards the apex, (7.8-)12-25.4(-34.7) μm long, (1.3-)1.6-2.6(-3.5) μm wide at the base, apical part forming conidia by sympodial proliferation on swollen a cluster of conidium-bearing faintly developed denticles, (1-)1.2-2.5(-3.3) μm long and (1.1-)1.4-2.9(-4.7) μm wide, denticles sometimes arise directly from hypha. Conidia abundant in cultures hyaline, unicellular, smooth, guttuliform, ellipsoid, sometimes curved, slightly pointed at the base (3.4-)4.2-6.6(-8.7) × (1.3-)1.9-3.1(-3.9) μm.

Culture characteristics.

Colonies with optimal growth at 25 °C on 2% MEA reaching an average of 36.3 mm ( ± 0.62 mm) after 14 days, with radial growth rate 1.05 ( ± 0.02) mm/d, growth somewhat slower at 30 °C (29.6 mm diameter); white, flat, floccose, growing in a circular pattern with entire margins.

Host tree.

Pinus sylvestris .

Insect vector.

Tomicus spp.

Distribution.

Known only from the type location (Poland).

Additional specimen examined.

Poland, Podkarpackie Province, Mielec, from fallen shoots of Scots pine pruned by Tomicus sp., October 2007, R. Jankowiak, (O-F-259434, culture CBS 147971).

Notes.

This species is phylogenetically distinct from the other Sporothrix species based on the ITS, TUB 2, and CAL sequences. Sporothrix tumida grouped most closely with S. macroconidia (ITS, CAL) from which it can also be distinguished by dimensions of conidia (3.4-8.7 × 1.3-3.9 μm vs. 3.6-9.9 × 2.5-9.9 μm, Wang et al. 2019).

Figures

Figure 8. Sporothrix tumida sp. nov. (CBS 147970) a, b conidiogenous cell with an inflated cluster of denticles at the apex c denticles arising directly from hyphae d conidia e fourteen-day-old culture on MEA. Scale bars: 10 μm.