Lysobacter humi, Lee & Jang & Cha & Seo, 2017

Lee, Dongwook, Jang, Jun Hyeong, Cha, Seho & Seo, Taegun, 2017, Lysobacter humi sp. nov., isolated from soil, International Journal of Systematic and Evolutionary Microbiology 67 (4), pp. 951-955 : 953-954

DESCRIPTION OF LYSOBACTER HUMI SP. NOV.

Lysobacter humi (hu′ mi. L. gen. n. humi of soil).

Cells are Gram-reaction-negative, strictly aerobic, nonmotile, non-flagellated and rod-shaped with dimensions in the range 0.7–1.0×1.3–1.5 µm. Colonies on R2A are round, convex, transparent and yellow in colour after incubation at 30 Ǫ C for 2 days. The growth ranges by temperature, pH and NaCl concentration are 20–42 Ǫ C, pH 7.0–9.0 and 0– 0.5 % (w/v) NaCl, respectively, with optimum growth at 30 Ǫ C, pH 8.0 and 0 % (w/v) NaCl, respectively. Growth occurs on nutrient agar and R2A agar. Oxidase and catalase tests are positive. According to API 20NE, cells are positive for gelatin hydrolysis and aesculin hydrolysis, but negative for nitrate reduction, glucose fermentation, arginine dihydrolase activity, indole production and urea hydrolysis. Cells show activities for acid phosphatase, alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, lipase (C14), naphthol-AS-BI-phosphohydrolase, trypsin, valine arylamidase and a- chymotrypsin. Cells show no activity for cystine arylamidase, N -acetyl-b- glucosaminidase, a- fucosidase, a- galactosidase, a- glucosidase, a- mannosidase, b- galactosidase (ONPG), b- glucosidase or b- glucuronidase. Cells do not assimilate the following compounds: acetate, adipate, alanine, capric acid, citric acid, D- glucose, D- mannitol, D- mannose, D- sorbitol, gluconate, glycogen, inositol, itaconic acid, lactic acid, L- arabinose, L- fucose, L- histidine, L- rhamnose, L- serine, malic acid, malonic acid, maltose, melibiose, N -acetylglucosamine, phenylacetic acid, p -nitrophenyl-b- D- galactopyranoside (PNPG), potassium 2-ketogluconate, proline, propionic acid, ribose, salicin, suberic acid, sucrose, valeric acid, 3- hydroxybenzoic acid, 3-hydroxybutyric acid, 4-hydroxybenzoic acid or 5-ketogluconate (API 20 NE and API ID 32GN). The only isoprenoid quinone is ubiquinone Q-8. The most abundant cellular fatty acids are iso-C 16: 0, summed feature 9 (iso-C 17: 1 Ɯ 9 c and/or C 16: 0 10-methyl) and iso-C 15: 0. Polar lipids comprise phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and two unidentified aminophospholipids.

The type strain, FJY8 T (= KCTC 42810 T = JCM 31019 T), was isolated from an arid area in Goyang, Gyeonggi Province, South Korea. The G +C content of genomic DNA of the type strain is 68.0±0.4 mol%.