Miogryllus piracicabensis Piza, 1960

Orsini, Marcelo Pinheiro, Costa, Maria Kátia Matiotti Da, Szinwelski, Neucir, Martins, Luciano De Pinho, Corrêa, Robson Crepes, Timm, Vitor Falchi & Zefa, Edison, 2017, A new species of Miogryllus Saussure, 1877 and new record of Miogryllus piracicabensis Piza, 1960 (Orthoptera: Gryllidae) from State of Rio Grande do Sul, Brazil, with calling song and chromosome complement, Zootaxa 4291 (2), pp. 361-372 : 367-370

Miogryllus piracicabensis Piza, 1960

Miogryllus piracicabensis Piza, 1960 . Studia Entomolica, 3, 253–56. Type locality: Brazil, State of São Paulo, municipality of Piracicaba.

Examined Material. Brazil, State of Rio Grande do Sul, municipality of Capão do Leão (31o48’1.354’S, 52o25’5.833”W), 38 adult males, 9 adult females ( MZUSP), October to November, 2007 and from October to April, 2015-2016, E. Zefa & M. P. Orsini, leg.

External morphology ( Figs 21 , 23–28 ). Similar to M. itaquiensis n. sp., differing in the following characteristics: head slightly wider than pronotum; ecdysial suture whitish; presence of a diagonal line in the fifth palpomere slightly darkened; mandible light brown; considering 38♂♂ and 9♀♀, the marks in the pronotum lateral lobe vary in the color pattern, with 75.6% of the individuals showing these mark entirely whitish, 15.6% anteroventral whitish and light brown posteriorly, 6.7% antero-ventral light brown and whitish posteriorly ( Fig. 6 ), and 2.1% entirely light brown; outer auditory tympanum always present, bigger than occurs in M. itaquiensis n. sp.; inner auditory tympanum varying as 56% absent, 40% very smaller than outer or vestigial, 4% one third smaller than outer; subapical spurs number varying from 5 to 7 outer and from 4 to 6 inner.

Phallic sclerites ( Figs 29–31 ). Idem M. itaquiensis n. sp. including same variations.

Right tegmen ( Fig. 32 , n=41). Similar to M. itaquiensis n. sp., however apical region is reduced.

Measurements: harp area, 5.4 ± 0.72mm 2 (3.7–6.8); mirror area, 2.1 ± 0.38mm 2 (1.3–3); dorsal field length, 6.7 ± 0.7mm (4.9–7.9); dorsal field width, 2.9 ± 0.4mm (2.3–4,6); lateral field length, 6.5 ± 0.6mm (5.2–7.9); lateral field width, 2.6 ± 0.36mm (1.8–3.7); file length, 1.8 ± 0.17mm (1.3–2.1); teeth number, 97.5 ± 5.2 teeth (87–106); six accessory veins in lateral field.

Calling song ( Figs 19–20 , Tab. 1). Calling song emitted only in chirps; 4 to 6 pulses per chirp (percentage in the Table 1); chirp rate, 4.8 ± 0.65s (3.9–6, n = 30); chirp period, 0.20 ± 0.026s (0.17–0.25, n = 30); inter-chirp interval, 0.13 ± 0.03s (0.09–0.17, n = 30); dominant frequency 5910 ± 188Hz (5618–6134, n = 30); temperature from 17 to 25°C.

Female ( Figs 22 , 33–34 ). Body shape and color pattern similar to males, slightly bigger than male; short tegmina, covering two first abdominal tergites; subgenital plate trapezoid; ovipositor slightly longer than femur III, apex lanceolated.

Measurements (mm). Male (n=38): body length, 15.2 ± 1.44 (11.9–18); pronotum width, 4.3 ± 0.27 (3.6–5); pronotum length, 2.3 ± 0.18 (1.8–2.7); head width, 4.2 ± 0.3 (3.4–4.9); interocular distance, 3.3 ± 0.33 (2.5–3.9); femur III length, 8.8 ± 0.61 (7.6–10.3); tibia III length, 5.9 ± 0.41 (5.2–6.9). Female (n = 9): body length, 16.5 ± 2.64 (13.6–20.1); pronotum width, 4.8 ± 0.36 (4.2–5.2); pronotum length, 2.7 ± 0.26 (2.4–3.1); head width, 44.6 ± 0.24 (4.2–4.8); interocular distance, 3.4 ± 0.3 (3.0–3.8); femur III length, 9.7 ± 0.5 (9.1–10.6); tibia III length, 6.4 ± 0.38 (5.6–7.0); dorsal field length, 3.3 ± 0.6 (2.1–4.2); dorsal field width, 2.0 ± 0.3 (1.4–2.6); ovipositor length, 11.5 ± 1.6 (8.8–13.6).

Karyotype ( Fig. 36 ). Diploid chromosome number of 2n = 24 + X ♂ = 25, and 2n = 24 + XX ♀ = 26; three pairs of metacentric chromosomes (pair 1, ci = 43.1, with secondary constriction in p arm, which left a small satellite at the chromosome tip; pair 2, ci = 44.7; pair 4, ci = 44.4), five pairs of submetacentric (pair 3, ci = 34.2; pair 5, ci = 15.1; pair 6, ci = 29; pair 7, ci = 14.8; pair 10, ci = 17.1) and four pairs of acrocentric (pair 8, ci = 10.3; pair 9, ci = 11.5; pair 11, ci = 11.1; pair 12, ci = 10). The X chromosome is metacentric (ci = 43.4), and the largest of the complement.

Figures

FIGURES 21 – 22. Habitus of Miogryllus piracicabensis Piza, 1960 in vivo. 1 — male, 2 — female.

FIGURES 23 – 28. Male morphological characteristics of Miogryllus piracicabensis Piza, 1960. 23, 24, 25 — habitus after alcohol fixation, in dorsal, ventral and lateral views, respectively; 26 — head, lateral view; 27 — supranal plate; 28 — subgenital plate.

FIGURES 3 – 11. Morphological characteristics of Miogryllus itaquiensis n. sp. holotype. 3 – 5 — habitus after alcohol fixation, in dorsal, ventral and lateral views, respectively; 6 — head, lateral view; 7 — supranal plate; 8 — subgenital plate; 9 — 11 — phallic sclerites, ventral, dorsal and lateral view, respectively. Conventions: MLPs—main lobe of pseudepiphallus; Ect. F—Ectophallic Fold; Ect. Ap. — Ectophallic Apodeme; Arc—Ectophallic Arc; Ps. P— Pseudepiphallus Paramere; Ps. Ap—Pseudepiphallic Apodeme; End. C—Endophallic Cavity; R—Ramus.

FIGURES 29 – 34. Genitalia, right tegmen and ovipositor of Miogryllus piracicabensis Piza, 1960. 29 — 31 — phallic sclerites, dorsal, ventral and lateral view, respectively; 32 — male right tegmen; 33 — supranal plate, cerci and ovipositor; 34 — subgenital plate, cerci and ovipositor. Conventions: MLPs—Main Lobe of Pseudepiphallus; Ps. Ap. — Pseudepiphallic Apodeme; Ect. F— Ectophallic Fold; Arc—Ectophallic Arc; Ps. P— Pseudepiphallus Paramere; Ps. Ap—Pseudepiphallic Apodeme; End. C— Endophallic Cavity; R—Ramus.

FIGURES 17 – 20. Calling songs of Miogryllus itaquiensis n. sp. and Miogryllus piracicabensis Piza, 1960. 17 — 18 — Holotype oscillogram and spectrogram, respectively; 19 — 20 — oscillogram and spectrogram of Miogryllus piracicabensis.

FIGURES 35 – 36. Karyotype of Miogryllus. 35 — Miogryllus itaquiensis n. sp.; 36 — Miogryllus piracicabensis Piza, 1960.