Limosilactobacillus rudii, Li & Cheng & Zheng & Liu & Quevedo & Li & Roos & Gänzle & Walter, 2021

Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, International Journal of Systematic and Evolutionary Microbiology (004644) 71 (2), pp. 1-21 : 17

DESCRIPTION OF LIMOSILACTOBACILLUS RUDII SP. NOV.

Limosilactobacillus rudii [ru′ di.i.i. N.L. gen. n. rudii of Rudi (Vogel), in recognition of the German scientist Rudi F. Vogel, in recognition of his significant contributions to the taxonomy of lactic acid bacteria as well as the technology and microbial ecology of fermented foods].

Cells are Gram-positive, non-motile, non-spore-forming, catalase-negative and heterofermentative. Cells are rodshaped, measuring 1.1–2.7×0.7–1.2 µm. Colonies of the type strain STM3_1 T on MRS agar plate incubated under the anaerobic condition at 37 °C for 2 days are whitish, opaque, raised, circular and entire, with a diameter of 1.0– 2.2 mm; colonies on MRS agar plate at the aerobic condition show similar morphological characteristics as colonies on MRS agar incubated anaerobically, but with a smaller diameter of 0.4–0.8 mm. D-Lactate, L-lactate and gas are produced from glucose fermentation by the type strain STM3_1 T. Cell growth occurs at 30 and 37 °C (optimum), but not at 15 or 45 ° C. Growth occurs at pH 4.0–8.0 in MRS broth. The major fatty acid of STM3_1 T is C 16:0, followed by summed feature 7 (combination of C 19:1 ω 6 c and/or C 19:0 cyclo ω 10 c) and C 19:0 cyclo ω 8 c. Acid is produced from L-arabinose, D-ribose, D-xylose, D-galactose, D-glucose, aesculin, maltose, lactose, melibiose, sucrose and raffinose; acid is not produced from D-fructose,D-mannose, methyl α- D-glucopyranoside, potassium gluconate, glycerol, erythritol, D-arabinose,L-xylose,D-adonitol, methyl β - Dxylopyranoside, L-sorbose,L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cell-wall peptidoglycan of STM3_1 T contains the amino acids alanine (Ala), glutamic acid (Glu), lysine (Lys), aspartic acid (Asp), serine (Ser) and threonine (Thr), with the molar ratio 1.1 (Ala):1.0 (Asp):1.0 (Glu):0.8 (Lys):0.1 (Thr), suggesting the cell-wall peptidoglycan type A4α L-Lys–D-Asp by [ 38]. The DNA G+C content of STM3_1 T is 38.5mol%.

The type strain, STM3_1 T (=DSM 110572 T =LMG 31631 T), was isolated from the faecal sample of striped mouse ( Rhabdomys pumilio ) raised at Henry Doorly Zoo and Aquarium (Omaha, NE, USA) .