Limosilactobacillus balticus, Li & Cheng & Zheng & Liu & Quevedo & Li & Roos & Gänzle & Walter, 2021

Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, International Journal of Systematic and Evolutionary Microbiology (004644) 71 (2), pp. 1-21 : 13-16

DESCRIPTION OF LIMOSILACTOBACILLUS BALTICUS SP. NOV.

Limosilactobacillus balticus (bal.ti′ cus. L. adj. balticus pertaining to the Baltic region where the type strain was isolated).

Cells are Gram-positive, non-motile, non-spore-forming, catalase-negative and heterofermentative. Cells are rod-shaped, measuring 0.9–3.0×0.6–1.0 µm. Colonies of the type strain BG-AF3-AT on MRS agar plate incubated at the anaerobic condition at 37 °C for 2 days are whitish, opaque, raised, circular and entire, with a diameter of 1.2–3.2 mm; no colony appears on MRS agar plate incubated at the aerobic condition at 37°C for 2 days. D-Lactate, L-lactate and gas are produced from glucose fermentation by the type strain BG-AF3-AT. Growth occurs at 30, 37 and 45 °C (optimum), but not at 15 ° C. Growth occurs at pH 4.0–8.0 in MRS broth. For BG-AF3-AT, the most abundant fatty acid is C 16:0, followed by C 19:0 cyclo ω 8 c. Acid is produced from L-arabinose, D-ribose,D-xylose,D-galactose,D-glucose, aesculin, maltose, lactose, melibiose, sucrose and raffinose; acid is not produced from D-fructose,D-mannose, methylα- D-glucopyranoside, potassium gluconate, glycerol, erythritol, D-arabinose, L-xylose,D-adonitol, methyl β -D-xylopyranoside,L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol,D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose,D-fucose,L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cellwall peptidoglycan of BG-AF3-AT contains the amino acids alanine (Ala), glutamic acid (Glu), lysine (Lys), and aspartic acid (Asp), with the molar ratio 1.3 (Ala):0.8 (Asp):1.0 (Glu):1.0 (Lys), suggesting the cell-wall peptidoglycan type A4α L-Lys–D-Asp by [ 38]. The DNA G+C content of BG-AF3-AT is 38.3mol%.

Fatty acid (%) L. balticus sp. nov L. agrestis sp. nov. L.. nov. L. rudii . nov. L. fastidiosus sp. nov. L. reuteri L. vaginalis BG-AF3- AT pH52_RY WF-MT5- AT BG-MG3-A Lr3000 _1_1 STM3_1 T STM2_1 WF-MO7-1 T WF-MA3-C DSM 20016 T DSM 5837 T C14:0 2.6 3.6 1.8 5.3 0.5 0.6 1.8 2.0 7.6 7.2 2.3 7.4 iso-C15:0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.2 0.0 0.0 iso-C16:0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.7 0.0 0.0 C16:0 42.3 32.4 48.3 50.4 16.5 16.9 42.2 46.0 33.4 30.2 37.4 23.7 C17:0 cyclo 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.0 0.0 0.0 C16:0 3-OH 0.0 0.4 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.6 0.0 0.0 C18:1 ω 9c 6.4 21.7 5.7 7.3 31.4 30.1 6.5 6.6 17.2 21.2 6.7 33.3 C18:0 3.0 2.5 5.0 2.5 3.3 3.3 5.3 5.7 1.7 2.2 7.1 0.0 C18:1 ω 7c 11-methyl 0.0 0.0 0.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 C17:0 3-OH 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.3 0.0 0.0 0.0 0.0 iso-C19:0 1.0 1.3 0.4 0.0 2.3 2.5 1.3 1.2 0.0 1.0 0.0 0.0 C19:0 cyclo ω 8 c 18.9 8.4 20.4 13.6 0.0 0.0 14.2 15.7 0.0 0.0 7.0 0.0 Summed feature 3† 1.5 1.7 1.5 1.9 1.2 1.3 1.3 1.5 2.7 2.0 3.0 4.4 Summed feature 5† 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 17.1 0.0 Summed feature 7† 14.5 19.3 13.8 15.1 36.8 38.4 20.6 21.2 31.4 30.1 5.4 15.7 Summed feature 8† 9.9 8.7 2.6 4.0 8.1 7.0 7.0 0.0 5.2 4.6 13.9 15.5

albertensis sp sp T RRLNB

The type strain is BG-AF3-AT (=DSM 110574 T =LMG 31633 T), which was isolated from the jejunum of yellow-necked mouse ( Apodemus flavicollis ) caught in the Vilnius area in Lithuania [ 13].