Pestalotiopsis licualicola K. Geng, Y. Song, K.D. Hyde & Yong Wang bis, Phytotaxa 88 (3):51. (2013)

Zhang, Zhaoxue, Liu, Rongyu, Liu, Shubin, Mu, Taichang, Zhang, Xiuguo & Xia, Jiwen, 2022, Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China, MycoKeys 88, pp. 171-192 : 171

Pestalotiopsis licualicola K. Geng, Y. Song, K.D. Hyde & Yong Wang bis, Phytotaxa 88 (3):51. (2013)

Fig. 5

Description.

Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on PDA: Conidiomata solitary, scattered, black, raising above surface of culture medium, subglobose. Conidiophores cylindrical, hyaline, smooth-walled. Conidiophores often indistinct. Conidiogenous cells discrete, hyaline, simple, filiform, 5.5-10.0 μm long. Conidia 18.0-24.5 × 4.0-5.5 μm, mean ± SD = 20.5 ± 1.9 × 5.3 ± 0.3 μm, fusiform, straight to slightly curved, 4-septate, smooth, greyish brown; basal cell conical, hyaline, thin-walled, 2.8-6.0 μm long; with three median cells, dark brown, concolorous, septa and periclinal walls darker than the rest of the cell, together 11.5-16.0 μm long, mean ± SD = 13.2 ± 1.2 μm; second cell from base 3.4-5.5 μm; third cell 3.3-4.7 μm; fourth cell 3.5-5.1 μm; apical cell hyaline, conic to subcylindrical, 3.1-5.3 μm; with 1-3 tubular apical appendages (mostly 1) without knobs, arising from the apex of the apical cell, 10.0-20.5 μm long, mean ± SD = 16.0 ± 4.0 μm; basal appendage filiform, short.

Culture characteristics.

Colonies on PDA reaching 70.0-80.0 mm diam after 7 d at 25 °C, growth rate 9.0-12.0 mm/day, edge entire, whitish to pale honey coloured, with sparse aerial mycelium on the surface, with black, gregarious conidiomata; reverse similar in colour.

Specimen examined.

China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Ilex chinensis , HSAUP210087, living culture SAUCC210087; on diseased leaves of Ilex chinensis , HSAUP210088, living culture SAUCC210088.

Notes.

In the present study, two strains (SAUCC210087 and SAUCC210088) from symptomatic leaves of Ilex chinensis were clustered to Pestalotiopsis licualicola clade ( Geng et al. 2013) based on phylogeny (Fig. 1 ). Morphologically, our strains were the same as P. licualicola , which was originally described with an asexual morph on leaves of Licuala grandis in China. The sexual morph of P. licualicola was undetermined yet. This is the first time this species has been reported in Ilex chinensis ( Aquifoliaceae ) in China.

Figures

Figure 1. Phylogram of Sporocadaceae based on combined ITS, tub 2 and tef 1 sequences. The BI and ML bootstrap support values above 0.90 and 70 % are shown at the first and second position, respectively. The tree is rooted to Bartalinia robillardoides (CBS 122705), ex-type or ex-epitype cultures are indicated in bold face. Strains from the current study are in red. Some branches were shortened according to the indicated mulipliers.

Figure 5. Pestalotiopsis licualicola (SAUCC 210087) a diseased leaf of Ilex chinensis b surface of colony after 7 days on PDA c reverse of colony after 7 days on PDA d conidiomata f, g, j, k conidiogenous cells with conidia e, h, i, l, m conidia. Scale bars: 10 μm (e-m).