Isoperla baumanni Szczytko & Stewart, 1984

Sandberg, John B., 2011, The Isoperla Of California (Plecoptera: Perlodidae); Larval Descriptions And A Key To 17 Western Nearctic Species, Illiesia 7 (22), pp. 202-258 : 216-219

Isoperla baumanni Szczytko & Stewart

NEW LARVA DESCRIPTION

( Figs. 1 a-d, 2 c, 5 a-h, 20 c)

Isoperla baumanni Szczytko & Stewart 1984 , 77:258-260. ♂, ♀, Ovum unknown.

Isoperla baumanni Szczytko & Stewart 2002 , 128:2-3. Larva (not reared) = I. bifurcata .

Material examined. TYPES: I. baumanni , Holotype ♂, Allotype ♀, CA: Plumas Co., Domingo Springs Campground, 6 mi (9.7 km) NW Chester, Domingo Spring, 25/VI/1980, R. Baumann, J. Stanger ( NMNH #104070). Additional Specimens. CALIFORNIA: Plumas Co., Domingo Spring, Domingo Springs Campground, 8.5 mi (13.7 km) NW Chester on Old Red Bluff Rd., 20/I/2007, J. Sandberg, Larvae; 04/II/2007, Larvae; 24/III/2007, Larvae; 09/VI/2007, J. Sandberg, A. Richards, Larvae (reared); 13/VI/2007, J. Sandberg, ♂ ♀, Larvae (reared); 17/VI/2007, ♂ ♀, Larvae (reared); 07/VII/2007, ♂ ♀ Larvae; 03/XI/2007, Larvae; 02/V/2008, J. Sandberg, D. Pickard, Larvae; 17/V/2008, J. Sandberg, Larvae; 13/VI/2008, Larvae (reared); 20/VI/2008, ♂ ♀ Larvae (reared); 22/VI/2008, ♂ ♀, Larvae; 05/VI/2010, Larvae (reared); 03/VII/2010, ♂ ♀.

Male larva. Body length of mature larva 9–10 mm. Dorsum of head with contrasting pigment pattern and fine dark clothing setae, anterior frontoclypeus margin unpigmented; light M shaped pattern anterior to median ocellus usually connected to light frontoclypeus area by an apically narrowed median longitudinal light band, its width at mid length approximately equal to the width at base, lateral arms with irregular margins usually disconnected from median light band, directed posterolaterally, and extending to antennal bases; posterior ocelli with partially enclosed large light areas along outer lateral margins; interocellar area partially light, completely enclosed by dark pigment, light area extending past posterior ocelli, not reaching dark pigment below the arms of the epicranial suture, light area generally oval shaped with acutely constricted base; occiput with irregular spinulae band extending from below eye to near median epicranial suture, completely enclosed by dark pigment ( Figs. 1d , 5a ). Lacinia bidentate, total length 1069–1256 µm ( Figs. 1 , 2c , 5e- h , Tables 2-4 ); submarginal row (A+B) with 7–10 setae, groups A-B interrupted by gap below subapical tooth (SAT) inner margin ( Fig. 5g ); 5–7 submarginal setae (A) in a close set row beginning at base of apical tooth (AT), ending before reaching SAT inner margin, row usually single, rarely 2 setae thick, plus 1 thin marginal setae (TMS) adjacent to AT inner margin, sometimes obstructed from view by the AT, submarginal setae (A), or broken, and 1 dorsal seta (DS) located below SAT inner margin, sometimes obstructed from view by SAT, submarginal setae (B), or broken ( Fig. 5h ); 2–4 submarginal setae (B) located past SAT inner margin ( Figs. 5 g-h); 15–27 marginal setae (C) initially longstout and widely spaced, usually several setae near end of row arranged in pairs protruding at dorsal and ventral angles, last few shorter and closer, blending into and difficult to differentiate from dorsal surface setae ( Fig. 5e ); 38–71 ventral surface setae (D) scattered below submarginal and marginal setae, ending posteriorly at approximately ¾ the inner lacinia margin length, occasionally a few setae below submarginal row striated ( Fig. 5f ); dorsal surface setae (DSS) forming dense, laterally protruding, longitudinal band, concentrated at junction with marginal setae (C), ending at approximately ½ or a little more the lacinia length ( Fig. 5e ). Galea with 15–33 setae in sparse ventral row, apex with 2–3 setae. Maxillary Palp segments 2–3 with curved apically rounded setae ( Fig. 5e Inset). Pronotum with broad median light area bordered by thick dark comma shaped bands typical of the I. sobria complex; discs each with thick black clothing setae, those along median margins usually enclosed by indistinct light brown pigment, lateral margins with broad light bands ( Fig. 5b ). Meso and metanotum with contrasting pigment pattern and fine dark clothing setae ( Fig. 5c ). Legs with numerous fine golden clothing setae and scattered erect spines on outer surface of femora, erect spines longest and concentrated along dorsal surfaces; fine silky setae sparse on dorsal surface of femora, numerous and continuous on tibia ( Fig. 20c ); tibia with faint transverse bands near proximal end. Abdominal terga with three longitudinal dark stripes; wide light median longitudinal band bisected with thin light brown median longitudinal stripe present on distal segments; lateral pair of dark longitudinal stripes about twice as wide as median dark stripe, extending to lateral margins; numerous fine dark clothing setae and erect spines scattered dorsally; posterior margin with scattered long and numerous short spines in a concentrated row ( Fig. 5d ).

Distribution. Northern California, known only from one Sierra Nevada high elevation spring.

Diagnosis. The 5–7 submarginal (A) setae of I. baumanni male larvae ( Figs. 5 g-h) are most similar to Isoperla sobria (Hagen) and I. tilasqua but differs from I. miwok with only one submarginal (A) seta ( Fig. 11g ). The setae counts alone were not sufficient to separate the three species with multiple close set submarginal (A) setae; I. sobria with 3–4 submarginal (A) setae ( Figs. 17e , g-h) and 2-5 submarginal (A) setae for I. tilasqua ( Figs. 19e , g-h). It is suspected that increased variation will be observed as more populations are studied. The basal segments 2–3 of the maxillary palpi have long, thin, apically rounded setae ( Fig. 5e Inset) which are shared by I. tilasqua ( Fig. 19e Inset); I. sobria has long thin, apically pointed setae ( Fig. 17e Inset). The variably shaped and large I. baumanni interocellar light area is completely enclosed by dark pigment ( Fig. 5a ), similar to I. sobria ( Fig. 17a ), but the latter is usually an inverted V shape (or sometimes a very small light spot). The interocellar light area of I. tilasqua (Meacham Creek, Oregon population) is not completely enclosed by dark pigment, and continues past the posterolateral ocelli to the median base of the dorsal head capsule ( Fig. 19a ). The light M shaped dorsal head pattern of I. tilasqua is a continuous line, but is interrupted in I. baumanni and I. sobria . A median longitudinal light band is present in all three species, but this line is approximately half as wide as the median base of the light M pattern in I. tilasqua ( Fig. 19a ).

Remarks. Isoperla baumanni may be restricted to Domingo Spring, Plumas County, a high volume and nearly stenothermic spring and emergence occurred in June–July. It was absent in nearby smaller Mosquito Spring containing both I. bifurcata and I. sobria , and an unnamed spring tributary to Gurnsey Creek with only I. bifurcata present. Recent collecting and rearing at Domingo Springs from 2006–2010 confirm three sympatric species: I. baumanni , I. bifurcata , and I. marmorata (three collected adults only). Isoperla bifurcata larvae were distributed throughout the spring and collected from all available submerged substrates (aquatic moss, aquatic plants, wood and rock). Isoperla baumanni larvae were primarily collected from submerged aquatic liverworts located in a small area along the southeastern bank, below the footbridge and above the cattle fence.

Everted I. baumanni males (reared & collected) did not compare well with the original description and appears to be the result of using two methods for everting the aedeagus. The aedeagus illustrated by Szczytko & Stewart (1984 – Fig. 53) was cleared and then hyper everted and this method may not be directly comparable to males that were everted live. Attempts to produce a similar evertion as illustrated in Szczytko & Stewart (1984) resulted in bursting the aedeagus membrane. In the current study, 31 reared and fully everted males lacked the projected, conical, anterodorsal striated, bladelike tip. Instead, a small depression was visible in this area and when the freshly everted aedeagus was fixed in near boiling water, seminal fluid streamed from the depression. The absence of long, paired membranous lobes was also observed in I. fulva , I. miwok , and I. roguensis , emphasizing the difference between live everted specimens of the current study and hyper everted preserved specimens illustrated in previous studies. Perhaps the striated bladelike tip of the I. baumanni aedeagus as illustrated in Szczytko & Stewart (1984) is an internal valve, which controls the release of sperm during copulation.

Two mature male larvae from Long Canyon Creek, El Dorado County, California were prepared for maxillae examination and tentatively identified as I. baumanni . The partly mature larvae with developing wing pads were collected on April 27 th, 2007, by Austin Brady Richards near Bendorf Spring. Both the males and females in the sample possessed an inverted V shaped light mark within the interocellar area, similar to I. sobria . One unsuccessful return trip by the author was conducted in early July, 2011, to attempt rearing and adult collection.

Figures

Fig. 1. Isoperla baumanni left ventral maxilla, abdominal sternites, and head, Domingo Spring, Plumas County, CA. 1a. Submarginal row groups (A) and (B), Marginal row (C), Ventral surface setae (D), Dorsal surface setae (DSS) not illustrated, Thin marginal seta (TMS), and Dorsal seta (DS) not included in submarginal row counts (Table 1). Maxilla measurements (red lines): Apical tooth length (ATL), Subapical tooth length (STL), Apical palm width (PWA), Palm angle (PA), Palm length (PL), Basal palm width (PWB), Galea length (GL), and Palp segment lengths (PL 1–5). 1b. Male and 1c. Female sterna. 1d. Dorsal head capsule: Anterior frontoclypeus area (a), Light M shaped pattern (b) and median longitudinal band (2 arrows), Lateral arms (c), Outer posterior ocellus light area (d), interocellar area (e) and length (arrow), and Epicranial suture (f). Bars = 1 mm.

Figs. 2a-q. Isoperla lacinia habitus, 40x, Bar = 500µm. 2a. I. acula; 2b. I. adunca; 2c. I. baumanni; 2d. I. bifurcata; 2e. I. denningi; 2f. I. fulva; 2g. I. laucki; 2h. I. marmorata; 2i. I. miwok; 2j. I. mormona; 2k. I. muir; 2L. I. pinta; 2m. I. quinquepunctata; 2n. I. roguensis; 2o. I. sobria; 2p. I. sordida; 2q. I. tilasqua.

Figs. 5a-h. NEW DESCRIPTION of Isoperla baumanni nymph and ventral maxilla, Domingo Spring, Plumas County, CA. Submarginal setae groups A and B, Marginal setae C, Ventral surface setae D, and Dorsal surface setae DSS. Fig. 5e Inset: Maxillary palp segment 2 with apically rounded long thin setae, 400x. White bars = 1 mm, 5e-f 100x, 5g 200x, and 5h 400x.

Figs. 20a-q. Isoperla front right leg habitus, Larvae, 32–40x, Bar = 1mm. 20a. I. acula; 20b. I. adunca; 20c. I. baumanni; 20d. I. bifurcata; 20e. I. denningi; 20f. I. fulva; 20g. I. laucki; 20h. I. marmorata; 20i. I. miwok; 20j. I. mormona; 20k. I. muir; 20L. I. pinta; 20m. I. quinquepunctata; 20n. I. roguensis; 20o. I. sobria; 20p. I. sordida; 20q. I. tilasqua.

Figs. 11a-h. Isoperla miwok nymph and ventral maxilla, Tributary of Campbell Creek, Butte County, CA. Submarginal setae groups A and B, Marginal setae C, Ventral surface setae D, Dorsal surface setae DSS, Dorsal seta DS, Thin marginal seta TMS. White bars = 1 mm, 11e-f 100x, 11g 200x, and 11h 400x.

Figs. 17a-h. Isoperla sobria nymph Fall River, Deschutes County, OR and ventral maxilla, Mosquito Spring Creek, Plumas County, CA. Submarginal setae groups A and B, Marginal setae C, Ventral surface setae D, Dorsal surface setae, Dorsal seta DS, and Thin marginal seta TMS. White bars = 1 mm, 17e-f 100x, 17g 200x, and 17h 400x.

Figs. 19a-h. Isoperla tilasqua nymph and ventral maxilla, Meacham Creek, Umatilla County, OR. Submarginal setae groups A and B, Marginal setae C, Ventral surface setae D, and Dorsal surface setae DSS. Fig. 5e Inset: Maxillary palp segment 3 with apically rounded long thin setae, 400x. White bars = 1 mm, 19e-f 100x, 19g 200x, and 19h 400x.