Ferrovibrio denitrificans

Cho, Ja Young, Baek, Kiwoon, Kim, Eui-Jin, Han, Ji-Hye & Choi, Seoni Hwang and Ahyoung, 2020, A report on 14 unrecorded bacterial species isolated from the Nakdong River, South Korea, Journal of Species Research 9 (2), pp. 77-84 : 78-79

Description of Ferrovibrio denitrificans 6SW1-49

Cells are Gram-staining-negative, flagellated, and rod-shaped. Colonies are circular, convex, and white colored

Incubation conditions 25 3 ° d, C d 25 C 3 °,, 4 C d ° 25 25 3 ° d C, 3 d °, C 25 C 3 30 ° d, 25 3 C ° d, 25, C ° 3 d C ° 25, d 3 d 25 °, C 3 ° 3 25 d C, d C ° 4, 25 C 10 25, ° d, C 25 d 3 °

Medium TSA R 2A 2A R A 2 R NA NA TSA TSA R A 2 TSA TSA A R2 M-R 2

A 2 R A

Similarity % (

) 99.0 98.9 99.5 99.8 99.1 99.0 99.4 99.9 99.3 98.8 98.8 99.8 99.4 98.8

related closely species

massiliensis denitrificans viscosa anguilliseptica fibrivorans barcinonensis nealsonii limigenitum ruber coerulea kafniensis paucihalophilus indicum buctense

. Most Reyranella Ferrovibrio Brevundimonas Pseudomonas Cellvibrio Paenibacillus Bacillus Chryseobacterium Pedobacter Actinomadura Leifsonia Aquidulcibacter Photobacterium Flavobacterium affiliations taxonomic Accession number MG 818310 16 MG 17 818311 MN 6 540834 MG 818309 15 540796 2 MN KU 360712 21 MN 5 540832 MG 818307 13 540836 9 MN 818308 14 MG 540803 4 MN 7 MN 540833 MN 10 540837 MN 540835 8 their ID BA BA BA BA BA BA BA BA BA BA BA BA BA BA and River NNIBR 2017301 2017301 2019643 2017301 2019643 2015296 2019643 2017301 2019643 2017301 2019643 2019643 2019643 2019643 NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR NNIBR Nakdong 21 19 18 from the ID Strain 2 - 3 SWB SW 49 - 1 03 5 R- SA - 35 1 SWB - SA N- 12 03 - 49 SA - 11 T-03 - 1 SWB 24 9 R- - SA 03 - 3 6 SWB SA 4 - 03 T- 03 - MK R-0.1 MR- - MK03 0.1 03 M- MK - isolated 5 6 19 5 19 BK- 19 19 19 19 19 19 strains freshwater bacterial Family Rhodospirillaceae f Ferrovibrio _ Caulobacteraceae Pseudomonadaceae Cellvibrionaceae Paenibacillaceae Bacillaceae Flavobacteriaceae Sphingobacteriaceae Thermomonosporaceae Microbacteriaceae Caulobacterales Vibrionales Flavobacteriales unrecorded of Order Rhodospirillales Sneathiellales Caulobacterales Pseudomonadales Cellvibrionales Bacillales Flavobacteriales Sphingobacteriales Streptosporangiales Micrococcales Caulobacterales Vibrionales Flavobacteriales Summary )) Table 1. Isolate Nakdong River (Sangju Nakdong River Mulgeum (after three days of incubation at 25°C on R2A. Positive for reduction of indole production and urease activities in API 20NE, but negative for reduction of nitrate reduction, glucose fermentation, arginine dihydrolase, esculin hydrolysis, gelatin hydrolysis, and β -galactosidase. Does not utilize D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-glucosamine, D-maltose, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate, and phenylacetic acid. Strain 6SW1-49 displays the highest 16S rRNA gene sequence similarity with Ferrovibrio denitrificans S3 T (98.9%). Strain 6SW1-49 (= NNIBR 2017BA17) was isolated from a freshwater sample of Nakdong River. The GenBank accession number of 16S rRNA gene sequence is MG818311.