Actinomyces qiguomingii, Zhu & Yang & Lu & Lai & Jin & Wang & Pu & Ren & Huang & Wu & Zhang & Xu & Xu, 2020

Zhu, Wentao, Yang, Jing, Lu, Shan, Lai, Xin-He, Jin, Dong, Wang, Xiaoxia, Pu, Ji, Ren, Zhihong, Huang, Ying, Wu, Xiaomin, Zhang, Xiaoyan, Xu, Jianqing & Xu, Jianguo, 2020, Actinomyces qiguomingii sp. nov., isolated from the Pantholops hodgsonii, International Journal of Systematic and Evolutionary Microbiology 70 (1), pp. 58-64 : 63

DESCRIPTION OF ACTINOMYCES QIGUOMINGII SP. NOV.

Actinomyces qiguomingii (qi.guo.ming′ i.i. N.L. gen. n. qiguomingii , named after Guoming Qi, a prominent microbiologist in the China CDC for his seminal contribution to Vibrio taxonomy and classification).

Cells are Gram-stain-positive, catalase- and oxidase-negative, non-motile rods (0.3–0.5×2.1–2.8 µm). The novel strains grow under anaerobic and in air plus 5% CO 2 condition. The isolates grow at 28–42 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 7.0) and at 0–3 % (w/v) NaCl (optimum, 1%). Colonies are milky white, circular, convex and about 1 mm on BHI-sheep blood agar after 72 h incubation under optimal growth conditions. The end products from glucose fermentation include ethanol and lactic acid. Nitrate is reduced and indole is not produced. When tested with API Rapid ID 32A and ZYM kits, the cells are positive for β -galactosidase, α -glucosidase, β -glucosidase, arginine arylamidase, proline arylamidase, phenylalanine arylamidase, leucine arylamidase, tyrosine arylamidase, glycine arylamidase, histidine arylamidase, alkaline phosphatase, valine arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase. Acid is produced at 48 h incubation from glycerol, D-arabinose,L-arabinose, D-ribose, D-xylose, D-galactose, D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, inositol, D-mannitol, D-sorbitol, amygdalin, arbutin, aesculin, salicin, cellobiose, maltose, lactose, melibiose, sucrose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose and potassium gluconate. The major polar lipids are diphosphatidylglycerol and phosphatidylinositol-mannoside. The main cellular fatty acid is C 16:0, followed by C 18:1 ω 9 c. The main quinones are MK-10 and MK-9. The whole-cell sugars contain rhamnose, ribose and glucose. The diagnostic amino acids of cell-wall peptidoglycan include alanine, lysine, glutamic acid and ornithine.

The type strain, 410 T (= CGMCC 1.16361 T = DSM 106201 T), isolated from a Tibetan antelope in Qinghai, PR China, has a G+C content of 67.4 mol%. The 16S rRNA gene and genome sequence accession numbers of strain 410 T are MG 650117 and CP025228 , respectively. Strain 553 is classified in this species .