Hasanuddinia pogonomyos, Smales, L. R., 2014

Smales, L. R., 2014, Gastrointestinal helminths (Cestoda, Chabertiidae and Heligmonellidae) of Pogonomys loriae and Pogonomys macrourus (Rodentia: Muridae) from Papua Indonesia and Papua New Guinea with the description of a new genus and two new species, Zootaxa 3889 (1), pp. 92-106 : 97

Hasanuddinia pogonomyos sp. nov.

( Figs 9–16 , 17–23 )

Type host. Pogonomys macrourus (Milne-Edwards) .

Site in host. Small intestine.

Material examined. Holotype male, allotype female, from Pogonomys macrourus 12 k north west Tep Tep, Madang, Papua New Guinea, coll. P. German 10. viii. 1992, BBM NG 105004B; paratypes 4 males, 4 females.

Other material examined. From Pogonomys macrourus Chimbu Province: 1 female, Yuro Village 5k south east Mount Karimu, coll. R. Speare 2. v. 1984 SAM AHC 47437; Morobe Province: 2 females, 3 males and pieces of worms, Mount Missim Vic. Wau, coll. P. Woolley 19. ii. 1982, BBM NG 56565; Sanduan Province: 2 pieces females, 2 males, Murum River below Wilbeite (3° 25´S, 142° 06´E), Torricelli Mountains, West Sepik, coll. T. Flannery 10. vi. 1988; Southern Highlands Province: 9 females, 2 males Mount Bosavi north north west slopes, coll. A.B. Mirza 10. v. 1973, 13. v. 1973, 15. v. 1973, BBM NG 103006, 103069, 103070, 103102, 103106, 103107.

From Pogonomys loriae Milne Bay Province: 5 males, 5 females, Munimum Village (9° 53´S, 149° 23´E), Aguan area, coll P. German 10. viii. 1992 AM W46846, 46847, 46849; 1 female, slopes near Kawaya Village (9° 53´S, 149° 22´E), Aguan area, coll. P. German 11. viii. 1992 AM W46848; 1 female, Garatin Ridge (9° 90´S, 149° 38´E), 3k north, 0.4k east Aguan, 2 females Waiep Creek (9° 90´S, 149° 40´E), 2.5k north 1.5k east Aguan, 2 females, 1 male Waiep Creek (9° 91´S, 149° 40´E), coll A. Engilis Jnr. 12. iii. 1985, 13. iii. 1985, BBM NG 109443A, 109495 109498; Southern Highlands Province: 1 female, 3 males, Doma Peaks 2400m, coll. A.B. Mirza and Bin Yuo 13. iv. 1978 105735B, C.

Etymology. The species name is derived from the host genus name.

Description. General: Relatively large coiled nematodes, males smaller than females; cephalic vesicle prominent, with numerous annulations, buccal capsule vestigial. Mouth opening triangular, with rudimentary lips; cephalic and labial papillae not seen, 2 lateral amphids. Oesophagus claviform, nerve ring not seen, deirids dome shaped, at about same level as excretory pore.

Synlophe: Longitudinal cuticular ridges extend from posterior margin of cephalic vesicle to anterior to bursa or vulva, 8–10, usually 9 or 10, in anterior, 9–11, usually 10, in mid body, 9–10 usually 9 in posterior; axis of orientation of ridges from right ventral to left dorsal, inclined about 70° to sagittal plane; 5–6 ridges dorsal side, 4–5 ventral side; ventral ridges 2’, 3’ largest in male, 3’, 4’ largest in female ridges 1–7 increasing in size; ridges reduced in size posteriorly.

Male: Body length 4.8–9.7 (6.25) mm, maximum width 92–161 (111.9). Cephalic vesicle 59.5–66.0 (60.9) long. Oesophagus 300–500 (400.5) long; nerve ring not observed; deirids 250, 370 (2 measurements); excretory pore 250–370 (302.5) from anterior end. Bursa slightly asymmetrical, right side larger, with dorsal cleft, dorsal lobe shorter than lateral lobes; pattern of rays 2–3. Dorsal trunk symmetrical, divided at about 1/2 length, terminal divisions, rays 9, 10 symmetrical; rays 8 arising from dorsal ray at about same level, right ray longer; rays 4, 5, 6 with common stem, rays 6 curve posteriorly 4, 5 curve anteriorly, rays 2, 3 more slender than rays 4, 5 diverge distally, recurved ventrally, reaching margin of bursa. Genital cone not prominent. Spicules equal, filiform 400–550 (514) long, tips not straight, pointed. Gubernaculum 42.5–60.0 (51.5) long.

Female: Body length 10.5–14.5 (12.5) mm, maximum width 114 – 190 (155). Cephalic vesicle 46.2–49.5 (48.1) long. Oesophagus 413–530 (483) long; nerve ring not observed; deirids 313–450 (378); excretory pore 420 (1 measurement) from anterior end. Ovejector monodelphic, vulva opens near posterior end 113–210 (196) from tail tip; vestibule longest element 100–160 (130), vagina 70–100 (81.5) long, with dorsal diverticulum, sphincter 50–70 (55.5) shorter than infundibulum 70–120 (98) long. Tail reflected ventrally, may have small prepuce, 45–55 (50.5) long, ending in blunt conical tip. Eggs thin shelled, ellipsoidal, 65–76.5 (69.7) by 35.0–37.5 (36.5), as measured in utero.

Remarks. Following the key of Durette-Desset (1983) the specimens described above are placed in the subfamily Nippostrongylinae . The characters of the synlophe, well developed pointed ridges with an axis of orientation passing through ventral right and dorsal left sides, carene absent, gradient in size of ridges, latero median on dorsal side and medio lateral on ventral side, ridges in mid to left ventral field hypertrophied and bursa, asymmetrical with right lobe larger and dorsal ray bifurcated in distal half, conform to the diagnosis of the genus Hasanuddinia (see Hasegawa & Syafruddin 1994b). Hasanuddinia pogonomyos sp. nov. can be distinguished from the type species H. maxomyos Hasegawa & Syafriddin, 1994 in having a synlophe of 9–11, usually 10 ridges on the mid body not 13, with 2 not 3 ventral ridges hypertrophied, in lacking tubercules and rugose area on the inner surface of the bursa, having longer spicules (400–680 compared with 260–290) and a vagina with a dorsal diverticulum. Hasanuddinia pogonomyos is a smaller worm than H. chiruromyos Smales, 2011 , the only other species known in the genus and can be distinguished from it by having fewer ridges in the synlophe (8–11, usually 10 compared with 10–12 ridges in the anterior and mid body; 9–10 usually 9 compared with 9–15 posteriorly), shorter spicules (400–680 compared with 630–750 long) with both tips bent not one hooked over the other, a vagina with a dorsal diverticulum and longer eggs (62.5–80.0 (68.9) compared with 55–67 (63)) ( Smales 2011a). Hasanuddinia pogonomyos can also be distinguished from the partially characterized Hasanuddinia sp. described from Lorentzimys nouhuysi Jentick by the number of ridges on the synlophe 10–11 on the mid body of H. pogonomyos compared with 16 for Hasanuddinia sp. and the length of the spicules 400–680 compared with 712 (Smales 2010).

Figures

FIGURES 9 – 16. Hasanuddinia pogonomyos n. sp. Male. 9. Cervical vesicle; 10. Transverse section, anterior body; 11. Spicule tips; 12. Transverse section, mid body; 13. Bursa; 14. Gubernaculum and genital cone, lateral view; 15. Transverse section, posterior body; 16. Dorsal ray. Abbreviations: d, dorsal; v, ventral; l, left; r, right; long arrow shows axis of orientation of synlophe. Scale bars in µm: 9, 11, 14, 16, 25; 10, 12, 13, 15, 50.

FIGURES 17 – 23. Hasanuddinia pogonomyos n. sp. Female. 17. Anterior end, lateral view; 18. Transverse section, anterior body; 19. Ovejector, egg in utero; 20. Transverse section, mid body; 21. Transverse section, posterior body; 22. Posterior end showing prepuce; 23. Posterior end female, ovejector with egg in ovejector. Abbreviations: d, dorsal; v, ventral; l, left; r, right; dd, dorsal diverticulum; long arrow shows axis of orientation of synlophe. Scale bars in µm: 17, 19, 20, 28, 50; 18, 20, 21, 25.