Amphichorda excrementa Hong Yu

Wang, Zhi-Qin, Zhao, Jing, Dong, Quan-Ying, Wang, Yao, Lu, Ying-Ling, Luo, Run & Yu, Hong, 2024, Multi-locus molecular phylogenetic analysis reveals two new species of Amphichorda (Bionectriaceae, Hypocreales), MycoKeys 106, pp. 287-301 : 287-301

Amphichorda excrementa Hong Yu bis, Z. Q. Wang, Q. Y. Dong & Y. Wang sp. nov.

Fig. 2

Etymology.

Refers to the excrement material from which this fungus was isolated.

Type.

China, Yunnan Province, Kunming City, Changchongshan Country Park, 11 July 2019, Hong Yu and Yao Wang (YHH AECCS 200777, holotype; YFCC AECCS 848, ex-type).

Description.

Sexual morph: Undetermined. Asexual morph: Colonies on PDA attaining a diameter of 42–44 mm after a month at 25 ° C, white to cream, with high mycelial density, cottony, with a yellow margin, reverse pale yellow. Hyphae branched, smooth-walled, septate, hyaline, 0.6–1.3 µm wide. Cultures readily produced phialides and conidia after 3 weeks on potato dextrose agar at room temperature. Conidiophores arising laterally from hyphae, cylindrical, straight or slightly curved, hyaline and occasionally branched. Phialides arising laterally from aerial hyphae, occasionally solitary, mostly in whorls of 2–3 on lateral branches from the mycelia, basal portion cylindrical or flask-shaped, usually curved, 4.1–13.9 × 1.3–2.1 µm, tapering abruptly towards the apex, have a distinctly thin neck. Conidia 1.7–3.0 × 1.2–2.5 µm, one-celled, smooth-walled, hyaline, globose to elliptical, single. Chlamydospores not observed.

Substrate.

Animal faeces.

Distribution.

China.

Commentary.

Phylogenetic analyses showed that Amphichorda excrementa formed a separate clade with statistical support from the BI posterior probabilities (PP = 1.00) and the ML bootstrap proportions (BP = 90 %) and was closely related to A. felina , A. yunnanensis and A. monjolensis . However, A. excrementa can be distinguished from three species by morphological differences. The phialides of A. excrementa were longer (4.1–13.9 × 1.3–2.1 µm) than those of A. felina (1.5–8.5 × 1.8–2.9 µm) and the conidia were smaller than those of A. felina (1.7–3.0 × 1.2–2.5 µm vs. 2.5–4.7 × 2–3.5 µm). The phialides of A. excrementa were longer (4.1–13.9 × 1.3–2.1 µm) than those of A. yunnanensis (4–12 × 1–4 µm) and the conidia were smaller than those of A. felina (1.7–3.0 × 1.2–2.5 µm vs. 2–5 × 2–4 µm). The conidia of A. monjolensis were longer than those of A. excrementa (2.8–3.7 × 1.8–2.9 µm vs. 1.7–3.0 × 1.2–2.5 µm).

Figures

Figure 2. Morphology of Amphichorda excrementa A, B colony character on PDA medium after 30 d (A obverse B reverse) C – F conidiophores, conidiogenous cells and conidia. Scale bars: 2 cm (A, B); 10 µm (C – F).