Perenniporiopsis srijayewardenepurana Thambugala & Karunarathna, 2023

Thambugala, Kasun M., Karunarathna, Samantha C., Zhao, Chang-Lin, Elgorban, Abdallah M., Tennakoon, Danushka S. & Konara, Ushari A., 2023, Perenniporiopsis srijayewardenepurana sp. nov. and validation of Pyrrhoderma nigrum in Sri Lanka, Phytotaxa 619 (2), pp. 123-136 : 130-131

Perenniporiopsis srijayewardenepurana Thambugala & Karunarathna sp. nov.

MycoBank no.: MB849640

Etymology: The specific epithet “srijayewardenepurana” refers to the University of Sri Jayewardenepura, one of the leading universities in Sri Lanka.

Synopsis: Differs from Perenniporiopsis minutissima by having 6–8cm size, yellowish brown rubbery pileus, 17.5–40 × 8–12.5 µm size basidia, and 12–15.5× 6.0–7.5 µm size basidiospores.

Basidiocarps annual, pileate; pileus solitary or imbricate, rubbery, bony upon drying, applanate, projecting up to 5.5 cm, 9 cm wide, and 2.5 cm thick at base; pileal surface yellowish orange, azonate, rough, without taste when fresh; margin yellowish brown to white, blunt; pore surface white when fresh, becoming pale white to cream when dry; pores round, 4–6 per mm; dissepiments thin, entire; context whitish to cream, firmly bony, azonate, up to 2 cm thick; tubes white to cream buff, firmly bony, up to 1.5 cm long. Hyphal system trimitic; generative hyphae thin-walled, hyaline, with clamp connections, rarely branched, 3–5 µm in diam; skeletal and binding hyphae thick-walled, hyaline, skeletal hyphae has a distinct lumen, rarely branched, interwoven, 4.5–7 µm in diam, binding hyphae thick-walled with a narrow lumen, frequently branched, interwoven, 1.5–2.5 µm in diam, CB– in the context, CB+ in the hymenophoral trama, unchanged in KOH; Cystidia and Cystidioles absent. Basidia clavate, with four sterigmata and a basal clamp connection, 17.5–40 × 8–12.5 µm; basidioles dominant, shape similar to basidia. Basidiospores oblong to ellipsoid, truncate, hyaline, thick-walled, smooth, dextrinoid, CB+, (10.5–)12–15.5(–17) × (5.5–)6.0–7.5(–9) µm, L = 13.0 µm, W = 7.0 μm, Q = 1.73–1.79 (n = 60/2).

L = mean spore length (arithmetic average of all spores), W = mean spore width (arithmetic average of all spores), Q = variation in the L/W ratios between the specimens studied, and n (a/b) = number of basidiospores (a) measured from given number (b) of specimens.

Specimens examined: Sri Lanka, Western Province, Gampaha District, Kadawatha, on a dead unidentified wood piece, 14.09.2022, Kasun Thambugala, KTSL016, ( USJ-H-119 , holotype), ibid., KTSL020 ( USJ-H-120 ) .

Notes: Perenniporiopsis srijayewardenepurana is described here as the second species of Perenniporiopsis . Perenniporiopsis minutissima differs from P. srijayewardenepurana in having orange-brown to dark reddish-brown rubbery basidiocarps, 18.5–30 × 8–13µm size basidia and 12–15 × 6.5–8 µm size basidiospores. In the phylogeny presented here, P. srijayewardenepurana formed a distinct and highly supported clade (BS/BPP = 100%/1.00) which is sister to P. minutissima ( Figure 1 ).

Figures

FIGURE 1. The phylogram generated from the maximum likelihood analysis of combined ITS and LSU sequences from Perenniporiopsis and related taxa (Final likelihood value of -20718.599316). The tree is rooted with Stereum hirsutum (NBRC 6520) and Heterobasidion annosum (PFC 5252). The ex-type strains are indicated in bold, and the new species are indicated in red. Bootstrap support values ≥65% from the maximum likelihood (ML) and Bayesian posterior probabilities (PP) values ≥0.90 are given above the nodes.