Tulostoma winterhoffii H. Schub. & P. Specht, in Specht and Schubert, Z. Mykol. 79(2): 421. 2015.

Jeppson, Mikael, Altes, Alberto, Moreno, Gabriel, Nilsson, R. Henrik, Yolanda Loarce,, Bustos, Alfredo de & Larsson, Ellen, 2017, Unexpected high species diversity among European stalked puffballs - a contribution to the phylogeny and taxonomy of the genus Tulostoma (Agaricales), MycoKeys 21, pp. 33-88 : 66-68

Tulostoma winterhoffii H. Schub. & P. Specht, in Specht and Schubert, Z. Mykol. 79(2): 421. 2015. Figures 5h, 16f, 20

≡ Tulostoma fimbriatum var. heterosporum J.E. Wright, Biblioth. Mycol. 113: 113. 1987. Holotype. THE NETHERLANDS, Wassenaar: ”Voorlinden”, 7 Oct. 1950, R.A. Maas Geesteranus 7513 (L!)*.

Holotype.

GERMANY, Rheinland-Pfalz: Hohfels near Grünstadt, 24 May 1987, W. Winterhoff (HAL).

Tulostoma winterhoffii was recently described from Germany. The holotype was not available for sequencing in this study but a paratype, provided by its authors, was sequenced and found to match sequence data of the type material of T. fimbriatum var. heterosporum . In macro-morphology (Figure 5h) T. winterhoffii strongly reminds of T. fimbriatum but important features to distinguish it are the verrucose-echinate spores (no anastomoses seen under SEM; Figure 16f) and the variation in spore size ( Wright 1987: up to 9.7 µm in diameter; Specht and Schubert (2013): (4.5-) 5.5-8.5 (-10.3) μm including ornamentation). Our own material shows a similar variation, averaging 5.7 - 6.1 µm, some spores reaching 9 µm. The same can be observed in the type collection of T. fimbriatum var. heterosporum (Figure 20). Based on morphology and sequence data, T. fimbriatum var. heterosporum must thus be considered a distinct species, for which the name T. winterhoffi has priority.

Habitat and distribution.

Mostly in dry and sandy habitats in exposed places, on more or less calcareous soils. Currently known from the Czech Republic, Denmark, France, Germany, Hungary, the Netherlands, Norway, Spain, and Sweden. In Scandinavia it reaches 60 ' N. The world distribution comprises Europe, Asia (Caucasus), and N. America (California) according to Wright (1987; as T. fimbriatum var. heterosporum ).

Specimens examined.

DENMARK, Sjælland: Greve, Hundige Strand, 1 Jan. 2015, S.O. Jensen, Atlasprojekt SOJ2105-699039 (C)*. BULGARIA, Nesebar: Slyanchev Brjag, 18 Sept. 1979, S. Jeppson. M. Jeppson 1359 (GB). GERMANY, Rheinland-Pfalz: Grünstadt, Asselheim, am Höllenberg, 12 Apr. 2013, ZFM79(2) (paratype, H. Schubert and P. Specht private herbarium)*. HUNGARY, Bács-Kiskun: Fülöpháza, Fülöpházai Homokbuckák, among Syntrichia in sand steppe vegetation, 11 Apr. 2006, L. Nagy, T. Gunnarsson, T. Knutsson, J. Jeppson, M. Jeppson 7761 (GB)*. NORWAY, Buskerud: Hurum, Nærnes, Grundviksveien, 3 May 2010, A-E. Torkelsen, G. Gulden, A. Karlsson, J. Nilsson, M. Jeppson 9537 (GB); Oslo: Græssholmen, 12 May 2006, A-E. Torkelsen, A. Karlsson, J. Nilsson, A.K. Wollan, A. Ryberg, M. Jeppson 7895 (GB, O); Munkedammen, N.M. Blytt 229 (O F-86871). SPAIN, Madrid: en césped, Dec. 1984, A.M. Moscardó (AH 11647, GenBank KC333060); Madrid, en un solar urbano, 1988, F.J. Martínez (AH 11656, GenBank KC333061). SWEDEN, Bohuslän: Tjärnö, Syd-Koster, Kilesand, sandgräshed med skalrik sand nära havet, 18 Oct. 1987, S. Jeppson, J. Jeppson, M. Jeppson 2379 (GB)*; Gotland: Ardre, Kungens sal, 22 Sept. 2000, J-O. Tedebrand (UPS).