Pristiphora albitibia (Costa, 1859)

Prous, Marko, Vikberg, Veli, Liston, Andrew & Kramp, Katja, 2016, North-Western Palaearctic species of the Pristiphora ruficornis group (Hymenoptera, Tenthredinidae), Journal of Hymenoptera Research 51, pp. 1-54 : 14

Pristiphora albitibia (Costa, 1859)

Nematus albitibia Costa, 1859: 21. Syntype(s) ♂ possibly in MZUN, not examined. Type locality: Sila Grande, Calabria, Italy.

Nematus puncticeps Thomson, 1863: 619. Syntypes ♀♂ in MZLU, examined. Type locality: Dalarne, Stockholm, Ostergöthland, Småland, and Skåne, Sweden.

Nematus agilis Zaddach in Brischke, 1884: 142. Primary homonym of Nematus agilis Cresson, 1880 [= Euura agilis (Cresson, 1880)]. 3 ♂♀ syntypes possibly destroyed ( Blank and Taeger 1998). Type locality: not specified, but probably in former East Prussia (now Kaliningrad Oblast of Russia, or Poland).

Pristiphora aterrima Lindqvist, 1977: 92, syn. n. Holotype ♀ (DEI-GISHym20896) in MZH, examined. Type locality: Tolyany, Usolje, Irkutsk, Russia.

Similar species.

Externally, the most similar species are P. armata , P. confusa , P. leucopus , P. opaca , and P. subopaca , from which it is best distinguished by the structure of the saw (Figs 41-42 ) and the penis valve (Fig. 78 ). On the inner surface of the lancet there are small spiny pectines (or dentes semicirculares) that reach the sclerora, which are absent in other similar species. The saw (Fig. 42 ) and external morphology of the holotype of Pristiphora aterrima Lindqvist, 1977 is not distinguishable from the studied P. albitibia specimens and therefore we synonymise Pristiphora aterrima with Pristiphora albitibia .

Genetic data.

Based on COI barcode sequences, P. albitibia belongs to its own BIN cluster (BOLD:ACH1762) (Fig. 1 ). The nearest neighbour (BOLD:AAL8277, P. astragali ?) is 2.06% different. Although there are no nuclear TPI sequences for any of the genetically closest (according to COI barcodes) species ( P. astragali and P. sootryeni ), the three sequenced specimens of P. albitibia are nearly identical to each other (one specimen differed by one nucleotide from the other two) and clearly different from the other sequenced species (Fig. 2 ).

Host plants.

Vicia cracca L. ( Stein 1885, as P. puncticeps ; Vikberg 2006), V. hirsuta (L.) Gray, V. tetrasperma (L.) Schreb. ( Kangas 1985, as P. puncticeps ), Vicia baicalensis Turcz., Vicia unijuga A. Br. ( Verzhutskii 1981, as P. puncticeps ).

Distribution and material examined.

Palaearctic. Specimens studied are from Estonia, Finland, Germany, Russia, and Sweden.

Figures

Figure 1. Maximum likelihood tree of Pristiphora ruficornis group based on cytochrome oxidase subunit I (COI) sequences (1078 bp). Specimens that had at least the full barcode sequence (658 bp) were included in the analysis. Branches with multiple specimen identification labels represent identical sequences, only one of which was used in the analysis. Numbers on the nodes show approximate likelihood-ratio test (aLRT) support values and bootstrap proportions (%, BP). Support values for weakly supported branches (aLRT <0.9 and / or BP <70) are not shown. The inset shows the tree with outgroup species. The scale bar shows the number of estimated substitutions per nucleotide position. An asterisk (*) indicates the specimens that we have not studied. AUT, Austria; CAN, Canada; CHN, China; DEU, Germany; ESP, Spain; EST, Estonia; FIN, Finland; FRA, France; GBR, United Kingdom; ITA, Italy; MAR, Morocco; NOR, Norway; PRT, Portugal; SWE, Sweden; USA, United States of America. NUMTs?, possible nuclear mitochondrial pseudogenes.

Figure 2. Maximum likelihood tree of Pristiphora ruficornis group based on triose-phosphate isomerase (TPI) sequences (alignment length 842 bp). Branches with multiple specimen identification labels represent identical sequences, only one of which was used in the analysis. Numbers on the nodes show approximate likelihood-ratio test (aLRT) support values and bootstrap proportions (%, BP). Support values for weakly supported branches (aLRT <0.9 and / or BP <70) are not shown. The scale bar shows the number of estimated substitutions per nucleotide position.

Figures 41 - 45. Lancets of Pristiphora albitibia subgroup. 41 P. albitibia DEI-GISHym 20944 42 P. aterrima holotype 43 P. astragali DEI-GISHym 80042 44 P. astragali PR. 365 VV 45 P. sootryeni PR. 366 VV.

Figures 77 - 86. Penis valves of Pristiphora ruficornis group. 77 P. appendiculata DEI-GISHym 31555 78 P. albitibia DEI-GISHym 20956 79 P. ruficornis PR. 462 VV 80 P. melanocarpa PR. 425 VV 81 P. ruficornis DEI-GISHym 19636 82 P. melanocarpa PR. 409 VV 83 P. armata PR. 465 VV 84 P. leucopus PR. 466 VV reared ex ovo from Tilia sp. 85 P. armata DEI-GISHym 80020 86 P. leucopus GBIF-GISHym 3246 (syntype of Nematus crassicornis Hartig).