Toxoplasma gondii subsp. isolation

Gamble, Amandine, Ramos, Raül, Parra-Torres, Yaiza, Mercier, Aurélien, Galal, Lokman, Pearce-Duvet, Jessica, Villena, Isabelle, Montalvo, Tomás, González-Solís, Jacob, Hammouda, Abdessalem, Oro, Daniel, Selmi, Slaheddine & Boulinier, Thierry, 2019, Exposure of yellow-legged gulls to Toxoplasma gondii along the Western Mediterranean coasts: Tales from a sentinel, International Journal for Parasitology: Parasites and Wildlife 8, pp. 221-228 : 224

3.3. T. gondii isolation and genotyping

The three necropsied gulls were seropositive for anti- T. gondii antibodies. Two individuals showed positive real-time PCR (heart and brain) with threshold values (CT) of 30.50 and 34.74 respectively for individuals 170329Lmic01 ES (from Ebro Delta, Spain) and 170402Lmic0111 (from Sidrière, France). One live strain was isolated from the individual originating from Ebro Delta, Spain, as indicated by the observation of T. gondii tissue-cysts under the microscope in brain samples from inoculated mice. This isolate was genotyped as type II (Appendix A, Table S5.1).