Pseudogymnoascus camphorae Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Pseudogymnoascus camphorae Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 16

Etymology.

Referring to the type strains first isolated from epiphytic soil of Cinnamomum camphora (Linn) Presl.

Type.

China: Guizhou Province, Guiyang City, South Campus of Guizhou University 26°42'21"N, 106°67'13"E, epiphytic soil of C. camphora , 8 Jul 2018, Z.Y. Zhang (HMAS 351901 holotype designated here, ex-type living culture CGMCC 3.20876 = GZUIFR 22.021) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 20-22 mm diam., white (6A1), slightly raised, flocculent, margin irregular, localised bulge, exudates and diffusible pigments absent; reverse dark brown (6F8) to light brown (6D4) from centre to margin. Colony on MEA 20-21 mm diam., white (5A1) to yellowish-white (4A2) from centre to margin, flocculent, nearly round, margin undulated, exudates and diffusible pigments absent; reverse yellowish-brown (5E8) to orange (5A7) from centre to margin. Colony on OA 19-20 mm diam., white (13A1), slightly raised, fluffy, nearly round, margin regular, exudates absent, diffusible pigments abundant, pewter; reverse reddish (13A2).

Hyphae branched, septate, hyaline, smooth, 1.0-3.0 μm diam. Conidiophores abundant, solitary, sometimes minimally differentiated from hyphae, hyaline, smooth, arising from erect hyphae, usually bearing verticils of two to four branches at an acute angle. Aleurioconidia pyriform, obovoid, with a broad truncated basal scar, 4.0-5.5 × 3.0-3.5 µm (av. 5.2 × 3.2, n = 50). Terminal aleurioconidia at the axis obovoid, clavate or irregular, solitary or two in chains, 6.0-10.0 × 3.0-3.5 µm (av. 8.7 × 3.2 μm, n = 50), with a broad truncated basal scar. Intercalary conidia subglobose, drum, obovoid, 3.0-4.0 × 2.0-3.0 µm (av. 3.5 × 2.6 μm, n = 50), sometimes separated by connective cells that undergo rhexolysis. Arthroconidia absent. Sexual morph unknown.

Additional specimens examined.

China: Fujian Province, Xiamen City, Wuyuanbay Wetland Park 24°51'52"N, 118°17'48"E, soil, 19 Aug 2019, Z.Y. Zhang, GZUIFR 22. 22.049 .

Notes.

Pseudogymnoascus camphorae was placed as a member of clade H (Fig. 14 ). P. camphorae is phylogenetically related to P. zhejiangensis and P. catenatus ; however, P. camphorae still forms a single clade. Morphologically, P. camphorae can be differentiated from P. catenatus by its absent arthroconidia ( Zhang et al. 2021b). P. camphorae is distinguished from P. zhejiangensis by the size and shape of its aleurioconidia (pyriform, obovoid, 4.0-5.5 × 3.0-3.5 µm vs. obovoid to globose, 2.5-4.5 × 2.5-4.0 µm, respectively) and terminal aleurioconidia (obovoid, clavate or irregular, solitary or two in chains, 6.0-10.0 × 3.0-3.5 µm vs. clavate, long obovoid, 5-9 × 2.5-4 µm, respectively) ( Zhang et al. 2021b).

Figures

Figure 14. Concatenated phylogeny of the ITS, LSU, EF 1 A, RPB 2 and MCM 7 gene regions of species in Thelebolaceae. Ninety-nine strains are used. The tree is rooted in Leuconeurospora pulcherrima (CBS 343.76) and Leuconeurospora sp. (15 PA 04 and 02 NH 04). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.7) and ML bootstrap values (≥ 70 %) are indicated along branches (PP / ML). Novel species are in blue and bold font, new isolates are in blue and " T " indicates type derived sequences.

Figure 16. Pseudogymnoascus camphorae (from ex-holotype CGMCC 3.20876) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-i conidiophores and conidia j conidia. Scale bars: 10 µm (d-j).