Doryctobracon whartoni Marinho and Penteado-Dias, 2017

Marinho, Cláudia F., Cônsoli, Fernando L., Penteado-Dias, Angélica Maria & Zucchi, Roberto A., 2017, Description of two new species closely related to Doryctobracon areolatus (Szépligeti, 1911) (Hymenoptera, Braconidae), based on morphometric and molecular analyses, Zootaxa 4353 (3), pp. 467-484 : 471-473

Doryctobracon whartoni Marinho and Penteado-Dias sp. nov.

( Figs 1 B–B 2 , 3 A , 4 A–F )

http://www.zoobank.org/NomenclaturalActs/4D24EABE-41BE-44E5-8AE6-AC27CD421D19 Doryctobracon sp. 1: Deus et al. 2010 (Pedra Branca do Amapari, AP, ex A. atrigona in Geissospermum argenteum ).

Diagnosis. This species differs from Doryctobracon areolatus ( Szépligeti, 1911) , by having the fore wing infuscate with a hyaline band near the base (restricted to the subbasal and anal cell) and a second transverse hyaline band on the apical half ( Figs 1 B1 , 3A ). Stigma yellow, veins and bristles in the hyaline band yellow, veins and setae in the infuscate band dark brown ( Fig 3A ). Doryctobracon adaimei sp. nov. also has a hyaline area, but it is rounded and is situated after the stigma (dark brown) and before the wing apex (in the middle of R1a). This spot does not reach the posterior margin of the wing and ends in the middle of the second subdiscal cell (2nd disc) ( Fig 3B ). In general, D. whartoni sp. nov. is more setose than D. areolatus and D. adaimei sp. nov. ( Figs 1B , 4A–D ). On the propodeum, laterally the anterior region of the areola, one short basal transverse keel projects to reach the lateral longitudinal keel, a characteristic also found in D. adaimei sp. nov. ( Fig 4D ); in D. areolatus two transverse keels reach the lateral longitudinal keel. Tegula yellowish orange (dark brown in D. areolatus ). Ovipositor apex with a dorsal node as in D. areolatus , but ventral serrations are much more developed ( Fig 4E ).

Description Female. Length of body, excluding ovipositor 6.0– 6.9 mm.

Head. 1.5–1.3× wider than long; 1.4–1.3× wider than width of mesoscutum; midridge polished, slightly elevated, narrow between toruli and wider toward clypeus; distance between toruli equal to distance from toruli to eye ( Fig 4A ). Head smooth and polished, with more hairiness concentrated in the clypeus, inner margins of eyes and gena. Antenna slightly longer than body, 7.7–9.5 mm in length, with 58 to 63 flagellomeres; first flagellomere about 0.9–1.2× longer than second; 1.7–1.9× longer than wide. Eyes large, 1.3–1.4× wider than high ( Fig 4C ); in dorsal view, eye width 1.7–1.9× greater than width of temples; malar space 0.3–0.4× height of eyes. Clypeus 2.9– 3.8× wider than high, sinuate, distinctly protruding with median lobe on ventral margin, polished, with sparse setae two to three times longer than those on face ( Fig 4A ).

Mesosoma. 1.4–1.5× longer than high; 1.9–2.1× longer than wide; 1.3-1.5× higher than wide. Pronotum not visible dorsally; median lobe of mesoscutum and lateral lobes smooth and bright, with few sparse bristles, setae concentrated mainly on margins of lateral lobes ( Fig 4B ); notaulus smooth, complete, deeper anteriorly, shallower posteriorly, reunited in a large and polished impression without midpit ( Fig 4B ); scutellar sulcus divided into two large pits by median longitudinal septum; scutellum smooth with small punctures and many setae around margins, posterior portion narrower or sharper than in D. adaimei sp. nov. and in D. areolatus ( Fig 4B ); propodeum densely setose with median anterior basal keel (0.11–0.15) and complete posterior areola ( Fig 4D ); laterally, in the anterior region of the areola, one short basal transverse keel extends to reach the lateral longitudinal keel, which is prominent and distinctly curved, from the posterior half of the propodeum ( Fig 4D ).

Wings. Fore wing 5.8–6.4 mm length; wide stigma 3.8–4.2× longer than wide, with vein r slightly projecting from its midpoint; (RS+M)a straight, 1.4–1.7× longer than 3RSa; 2RS 1.2–1.7× longer than 3RSa, 1.3–1.5× longer than 1m-cu and 1.7–2.0× longer than r-m; 3RSa 2.3–3.3× longer than vein r; 2M 1.8–2.3× longer than 3RSa; (RS+M)b absent; vein 1cu-a slightly sloping away from 1M for 0.23–0.27 its length. Fore wing infumate with hyaline stripes at base and in median portion. Stigma, veins and setae in hyaline stripe yellow, and veins and setae on infumate stripe dark brown. Hind wing 4.0– 4.4 mm length, infumate.

Metasoma. 2.1–2.7× longer than wide and 1.0–1.4× wider than high; T1 0.8–1.0× width at apex; T1 width at apex about 1.2–1.4× width at base; non-sculptured, smooth and bright; ovipositor about 6.0 mm long (twice metasoma length); ovipositor with one subapical dorsal node and developed ventral serrations ( Fig 4E ); ovipositor sheath with 4–5 rows of bristles ( Fig 4F ).

General coloration. Yellowish orange; first two pairs of legs bright yellow, third pair with femur, trochanter, and coxa yellow, tibia and tarsi dark brown; ovipositor yellow; antenna and ovipositor sheath dark brown; apex of mandibles black; fore wing infuscate with hyaline stripe, stigma yellow; veins and setae yellow in hyaline stripe, and dark brown in infuscate stripe ( Figs 1B1 , 3A ). Hind wings infumate, sometimes with hyaline stripes at the apex ( Fig 1B1 ).

Male. Similar to female, but dark brown on last tergite. Head, in dorsal view, 1.23–1.30× wider than mesoscutum, 1.49–1.58× wider than face. In dorsal view, eye 1.71–1.74× wider than temple; face 1.6–2.0× wider than high; malar space 0.40–0.48× height of eyes; clypeus 2.7–3.1× wider than high; antenna with 52 to 59 flagellomeres; first flagellomere 1.0–1.2× longer than second, 1.5–1.9× longer than wide. Mesosoma 1.9–2.1× longer than wide, 1.3–1.5× higher than wide; metasoma 2.0–3.0× longer than wide, 1.0–1.5× wider than high.

Type material. Holotype. Female (DCBU 270194), BRAZIL: Amapá, Pedra Branca do Amapari, 00°46’54.9”N and 051°57’01.2”W, 01.ii.2006, reared from fruit fly larva Anastrepha atrigona in “Quina” ( Geissospermum argenteum ), coll R. A. Silva. Paratypes, with same data as holotype, 1 female, (DCBU 270195), 2 males (DCBU 270196, DCBU 270197) and 1 female, 1 male (ESALQ).

Etymology. This species is named after Robert A. Wharton, in recognition of his outstanding contributions to the taxonomy of Braconidae .

GenBank accession numbers. Doryctobracon whartoni sp. nov. Amapá: FJ560534 (ITS2) and FJ560542 (28SD2).

Figures

FIGURE 1. Chromatic variation on the wings and legs. Doryctobracon areolatus (A-A2), Doryctobracon whartoni sp. nov. (yellow stigma – YS) (B-B2), Doryctobracon adaimei sp. nov. (Goiás) (C-C2), D. adaimei sp. nov. (Tocantins) (D-D2), D. adaimei sp. nov. (Amapá) (brown stigma – BS) (E-E2).(the figures are not on the same scale).

FIGURE 3. Fore wing. (A) Doryctobracon whartoni sp. nov. (yellow stigma); (B) Doryctobracon adaimei sp. nov. (brown stigma).

FIGURE 4. Doryctobracon whartoni sp. nov. head, front view (A), mesosoma, smooth notaulices, dorsal view (B), head and mesosoma, smooth mesopleura (C), setaceous propodeum, dorsal view (D), ovipositor apex (E), pattern of ovipositor sheath bristles (F).

FIGURE2. Landmarks on fore wing of Doryctobracon areolatus.1. base of veincostal; 2. Intersection of the parastigma, 1RS and wing margin; 3. Intersection of the end of stigma and R1a; 4. Intersection of the radial sector 3RSb and wing margin; 5. Intersection of vein 3M and wing margin; 6. Intersection of vein 3CU and wing margin; 7. Intersection of vein 3-1A and wing margin; 8. Intersection of the veins 3-1A and 2cu-a; 9. Intersection of the veins 2CUa, 3CU and 2cu-a; 10. Intersection of the veins 1-1A, 1cu-a and 2-1A; 11. Intersection of the veins 1-1A and base of wing; 12. Intersection of the veins M+CU, 1M and 1CU; 13. Intersection of the veins M+CU, 1cu-a and 1CU; 14 Intersection of the veins 2CUa, 1CU and 1m-cu; 15. Intersection of the veins 2M, r-m and 3M; 16. Intersection of the veins 1m-cu, (RS+M)a, 2RS and 2M; 17. Intersection of the veins 1M, (RS+M)a and 1RS; 18. Intersection of the veins 3RSa, 3RSb and r-m; 19. Intersection of the veins 3RSa, r and 2RS; 20. Intersectionof the vein r and base of stigma (venational terminologyaccording to Sharkey & Wharton 1997).