Clevelandella ananiasi, Kotyk & Bourland & Soviš & Méndez-Sánchez & Škaloud & Varadínová & Čepička, 2024

Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, Zoological Journal of the Linnean Society 202 (1), pp. 1-51 : 8

Clevelandella ananiasi sp.nov.

( Figs 3–5 ; Supporting Information, Table S4)

Description based on population þom ASS hosts þom Papua Neo Guinea: Small Clevelandella * size invivo 69–87 × 35–52 µm* usually about 77 × 41 µm; size in protargol preparations 44–67 × 25–34 µm* usually about 55 × 29 µm. Body proper dorsoventrally flattened; overall outline in ventral view asymmetrically spade-shaped* with prominent less lobe* anterior cell end bluntly pointed; peristomial projection joins body proper to right of midline. Less cell margin notched at base of peristomial projection forming a lobe that overhangs the peristomial projection for about one-fissh of its length. Macronucleus ellipsoidal ( Figs 3A * 4A) to inverted teardrop-shape ( Figs 3B * 4B–E* G)* anterior end rounded* posterior end acutely tapered* chromatin coarsely granular. Karyophore aưached to posterior margin of macronucleus ( Figs 3A * 4A* B). Micronucleus ellipsoidal (4.4 × 3.4 µm)* adjacent to anterior margin of macronucleus ( Figs 3B * 4G). Swims slowly.

Somatic ciliature consists of about 60 kineties* only anterior two-thirds of body properly ciliated ( Fig. 5A * B). Right sutural kinetofragments not confirmed* cannot be completely excluded due to suboptimal orientation of cells in protargol preparations.

Peristomial projection extends an average of 28% of total cell length. Lateral part of peristomial opening extends about three-quarters of length ( Figs 4A * F* 5A) of peristomial projection. Adoral zone usually extends about 52% of cell length* composed of an average of 23 membranelles. Fine structures of POM unrecognizable due to poor staining but visibly stichomonad at the posterior ( Fig. 5B–D ).

Occurrence: Clevelandella ananiasi sp. nov. was found in the hindgut of two Panesthiinae species from Papua New Guinea: ASS and STH from the same locality (Wanang 3). It occurred in 56% of ASS individuals regardless of age and sex. In STH the ciliate was present only in one juvenile host individual among 10 dissected insects. When present* the ciliate is typically abundant. In ASS* infection with C. ananiasi sp. nov. frequently co-occurs (60%) with C. sidi sp. nov. requiring special care in species identification.

Figures

Figure 3. Schematic drawings of Clevelandella ananiasi from life (A) and protargol impregnation (B–D). A* ventral view of a typical individual. B* ventral view showing the oral apparatus and nuclear structures. C* ventral infraciliature. D* dorsal infraciliature.AZM* adoral zone of membranelles; CPK* circumperistomial cilia; CV* contractile vacuole; HL* hyaline subcortical layer; K* karyophore; Ma* macronucleus; Mi* micronucleus; POM* paroral membrane; SK* somatic kineties. Scale bars:20 µm.

Figure 4. Clevelandella ananiasi sp. nov. from ASS. In vivo (A* B) and asser protargol impregnation (C–G). A* ventral view showing karyophore (white arrowhead)* contractile vacuole (asterisk)* posterior extent of somatic cilia (black arrowhead)* and cilia of circumperistomial kineties (black arrow). B* ventral view showing a more slender macronucleus* karyophore (white arrowhead)* contractile vacuole (asterisk)* and adoral membranelles (black arrowhead).C* ventral view showing excretory (black arrow). D* ventral infraciliature.E* dorsal infraciliature of same cell as (D). F* detail view of peristomial projection showing the peristomial overture (black arrowhead) and cilia of the circumperistomial kineties (white arrowheads).G* detail view showing position of micronucleus (white arrow). Ma* macronucleus. Scale bars:20 µm (A–C)* 10 µm (D–F)* 5 µm (G).

Figure 5. Clevelandella ananiasi sp. nov. (from ASS) in the scanning electron microscope. A* ventral view showing anterior margin of peristomial overture (white arrowhead)* cilia of adoral membranelles (black arrowhead)* and cilia of circumperistomial kineties (black arrow). B* posteroventral view showing posterior end of the right suture (black arrow)* the cytoproct with protruding cilia (white arrow)* the inconspicuous less suture (black arrowhead)* and the posterior end of the paroral membrane (white arrowhead). C* detail view of the peristomial overture showing adoral membranelles (black asterisk)* cilia of the posterior end of the paroral membrane (black arrows)* dikinetids of the posteriormost circumperistomial kinety (white arrowheads)* and cilia of the circumperistomial kineties (white arrow). D* detail [same cell as (B)] showing the single file of basal bodies comprising the posterior part of the paroral membrane (white arrowhead)* cilia of the paroral (black arrow)* and the less suture (asterisk). Scale bars: 20 µm (A* B)* 10 µm (C* D).